Lipopolysaccharide O-antigen biosynthesis in Shigella dysenteriae serotype 1: analysis of the plasmid-carried rfp determinant

1994 ◽  
Vol 16 (1) ◽  
pp. 53-64 ◽  
Author(s):  
S. Göhmann ◽  
P.A. Manning ◽  
C.-A. Alpert ◽  
M.J. Walker ◽  
K.N. Timmis
Keyword(s):  
Shigella Dysenteriae ◽  
O Antigen ◽  
Serotype 1

Core-linked LPS expression of Shigella dysenteriae serotype 1 O-antigen in live Salmonella Typhi vaccine vector Ty21a: Preclinical evidence of immunogenicity and protection

Vaccine ◽  
2007 ◽  
Vol 25 (33) ◽  
pp. 6167-6175 ◽  
Author(s):  
De Qi Xu ◽  
John O. Cisar ◽  
Manuel Osorio ◽  
Tint T. Wai ◽  
Dennis J. Kopecko
Keyword(s):  
Salmonella Typhi ◽  
Vaccine Vector ◽  
Shigella Dysenteriae ◽  
O Antigen ◽  
Serotype 1

scholarly journals Shigella dysenteriae ShuS Promotes Utilization of Heme as an Iron Source and Protects against Heme Toxicity

2005 ◽  
Vol 187 (16) ◽  
pp. 5658-5664 ◽  
Author(s):  
Elizabeth E. Wyckoff ◽  
Gregory F. Lopreato ◽  
Kimberly A. Tipton ◽  
Shelley M. Payne
Keyword(s):  
Bacillary Dysentery ◽  
Receptor Gene ◽  
Shigella Dysenteriae ◽  
Heme Binding ◽  
Anaerobic Conditions ◽  
Iron Source ◽  
Heme Transport ◽  
Serotype 1 ◽  
Increased Sensitivity ◽  
Heme Binding Protein

ABSTRACT Shigella dysenteriae serotype 1, a major cause of bacillary dysentery in humans, can use heme as a source of iron. Genes for the transport of heme into the bacterial cell have been identified, but little is known about proteins that control the fate of the heme molecule after it has entered the cell. The shuS gene is located within the heme transport locus, downstream of the heme receptor gene shuA. ShuS is a heme binding protein, but its role in heme utilization is poorly understood. In this work, we report the construction of a chromosomal shuS mutant. The shuS mutant was defective in utilizing heme as an iron source. At low heme concentrations, the shuS mutant grew slowly and its growth was stimulated by either increasing the heme concentration or by providing extra copies of the heme receptor shuA on a plasmid. At intermediate heme concentrations, the growth of the shuS mutant was moderately impaired, and at high heme concentrations, shuS was required for growth on heme. The shuS mutant did not show increased sensitivity to hydrogen peroxide, even at high heme concentrations. ShuS was also required for optimal utilization of heme under microaerobic and anaerobic conditions. These data are consistent with the model in which ShuS binds heme in a soluble, nontoxic form and potentially transfers the heme from the transport proteins in the membrane to either heme-containing or heme-degrading proteins. ShuS did not appear to store heme for future use.

scholarly journals The association of haemagglutination and adhesion with lipopolysaccharide of Shigella dysenteriae serotype 1

1991 ◽  
Vol 34 (5) ◽  
pp. 259-264 ◽  
Author(s):  
F. Qadri ◽  
S. Haq ◽  
S. A. Hossain ◽  
I. Ciznar ◽  
S. Tzipori
Keyword(s):  
Shigella Dysenteriae ◽  
Serotype 1

scholarly journals Stx-Producing Shigella Species From Patients in Haiti: An Emerging Pathogen With the Potential for Global Spread

2015 ◽  
Vol 2 (4) ◽  
Author(s):  
Miranda D. Gray ◽  
Susan R. Leonard ◽  
David W. Lacher ◽  
Keith A. Lampel ◽  
Meer T. Alam ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
The United States ◽  
Shigella Dysenteriae ◽  
Emerging Pathogen ◽  
Shiga Toxins ◽  
Shigella Species ◽  
Global Spread ◽  
Serotype 1 ◽  
Epidemiological Link

Abstract Shiga toxins (Stx) are commonly produced by Shigella dysenteriae serotype 1 and Stx-producing Escherichia coli. However, the toxin genes have been detected in additional Shigella species. We recently reported the emergence of Stx-producing Shigella in travelers in the United States and France who had recently visited Hispaniola (Haiti and the Dominican Republic). In this study, we confirm this epidemiological link by identifying Stx-producing Shigella from Haitian patients attending clinics near Port-au-Prince. We also demonstrate that the bacteriophage encoding Stx is capable of dissemination to stx-negative Shigella species found in Haiti, suggesting that Stx-producing Shigella may become more widespread within that region.

scholarly journals A Pediatric Cluster of Shigella dysenteriae Serotype 1 Diarrhea with Hemolytic Uremic Syndrome in 2 Families from France

2004 ◽  
Vol 38 (9) ◽  
pp. e96-e99 ◽  
Author(s):  
V. Houdouin ◽  
D. Catherine ◽  
P. Mariani ◽  
N. Brahimi ◽  
C. Loirat ◽  
...  
Keyword(s):  
Hemolytic Uremic Syndrome ◽  
Shigella Dysenteriae ◽  
Serotype 1 ◽  
Uremic Syndrome

scholarly journals Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

2005 ◽  
Vol 43 (7) ◽  
pp. 3522-3525 ◽  
Author(s):  
M. Jacques ◽  
J. Labrie ◽  
F. St. Michael ◽  
A. D. Cox ◽  
M.-A. Paradis ◽  
...  
Keyword(s):  
Actinobacillus Pleuropneumoniae ◽  
Outer Core ◽  
O Antigen ◽  
Serotype 1
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