Induction of NF-kappaB nuclear translocation in human respiratory epithelial cells by group A streptococci

2002 ◽  
Vol 33 (6) ◽  
pp. 307-313 ◽  
Author(s):  
Eva Medina ◽  
Dorothea Anders ◽  
Gursharan S. Chhatwal
Keyword(s):  
Epithelial Cells ◽  
Nuclear Translocation ◽  
Group A Streptococci ◽  
Respiratory Epithelial Cells ◽  
Group A ◽  
Human Respiratory Epithelial Cells ◽  
Nf Kappab ◽  
Respiratory Epithelial

scholarly journals Group A streptococci efficiently invade human respiratory epithelial cells.

1994 ◽  
Vol 91 (25) ◽  
pp. 12115-12119 ◽  
Author(s):  
D. LaPenta ◽  
C. Rubens ◽  
E. Chi ◽  
P. P. Cleary
Keyword(s):  
Epithelial Cells ◽  
Group A Streptococci ◽  
Respiratory Epithelial Cells ◽  
Group A ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial

scholarly journals Effect of Group A Streptococcal Cysteine Protease on Invasion of Epithelial Cells

1998 ◽  
Vol 66 (4) ◽  
pp. 1460-1466 ◽  
Author(s):  
Pei-Jane Tsai ◽  
Chih-Feng Kuo ◽  
Kuei-Yuan Lin ◽  
Yee-Shin Lin ◽  
Huan-Yao Lei ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Extracellular Proteins ◽  
Wild Type ◽  
Respiratory Epithelial Cells ◽  
Group A ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial ◽  
Type Strains

ABSTRACT Cysteine protease of group A streptococci (GAS) is considered an important virulence factor. However, its role in invasiveness of GAS has not been investigated. We demonstrated in this study that two strains of protease-producing GAS had the ability to invade A-549 human respiratory epithelial cells. Isogenic protease mutants were constructed by using integrational plasmids to disrupt thespeB gene and confirmed by Southern hybridization and Western immunoblot analyses. No extracellular protease activity was produced by the mutants. The mutants had growth rates similar to those of the wild-type strains and produced normal levels of other extracellular proteins. When invading A-549 cells, the mutants had a two- to threefold decrease in activity compared to that of the wild-type strains. The invasion activity increased when the A-549 cells were incubated with purified cysteine protease and the mutant. However, blockage of the cysteine protease with a specific cysteine protease inhibitor, E-64, decreased the invasion activity of GAS. Intracellular growth of GAS was not found in A-549 cells. The presence or absence of protease activity did not affect the adhesive ability of GAS. These results suggested that streptococcal cysteine protease can enhance the invasion ability of GAS in human respiratory epithelial cells.

scholarly journals Role of pili in adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells.

1988 ◽  
Vol 56 (6) ◽  
pp. 1641-1646 ◽  
Author(s):  
P Doig ◽  
T Todd ◽  
P A Sastry ◽  
K K Lee ◽  
R S Hodges ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Respiratory Epithelial Cells ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial

Effect Of Chitinase On Il-8 Production In Human Respiratory Epithelial Cells

2008 ◽  
Vol 121 (2) ◽  
pp. S205-S205
Author(s):  
J HONG ◽  
K LEE ◽  
K KIM ◽  
H JEE ◽  
M SOHN ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Respiratory Epithelial Cells ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial

scholarly journals Interaction ofMycobacterium aviumComplex with Human Respiratory Epithelial Cells

2000 ◽  
Vol 181 (3) ◽  
pp. 1189-1193 ◽  
Author(s):  
Venkata M. Reddy ◽  
Bhavna Kumar
Keyword(s):  
Epithelial Cells ◽  
Respiratory Epithelial Cells ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial
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