A Molecular Phylogeny of the Frog Genus Tomopterna in Southern Africa: Examining Species Boundaries with Mitochondrial 12S rRNA Sequence Data

2002 ◽  
Vol 22 (3) ◽  
pp. 407-413 ◽  
Author(s):  
Abeda Dawood ◽  
Alan Channing ◽  
James P. Bogart
Keyword(s):  
Molecular Phylogeny ◽  
Southern Africa ◽  
Sequence Data ◽  
Species Boundaries ◽  
12S Rrna ◽  
Rrna Sequence ◽  
Mitochondrial 12S Rrna

scholarly journals Case Report: An Eyelid Nodule Caused by Candidatus Dirofilaria hongkongensis Diagnosed by Mitochondrial 12S rRNA Sequence

2021 ◽  
Author(s):  
Suppapong Tirakunwichcha ◽  
Lalana Sansopha ◽  
Chaturong Putaporntip ◽  
Somchai Jongwutiwes
Keyword(s):  
Case Report ◽  
Histological Examination ◽  
External Surface ◽  
Histopathological Examination ◽  
Dirofilaria Repens ◽  
12S Rrna ◽  
Rrna Sequence ◽  
Immature Female ◽  
Upper Eyelid ◽  
Mitochondrial 12S Rrna

A 59-year-old female living in Rayong Province, eastern Thailand, presented with painless, right upper eyelid nodule for 3 months. Upon removal of the eyelid mass, a well-circumscribed, firm globular mass with diameter about 1 cm was found. Histopathological examination revealed an immature female dirofilarial worm reminiscent of Dirofilaria repens, characterized by prominent sharp longitudinal ridges at external surface of the cuticle. Analysis of the mitochondrial 12S rRNA sequence showed that the worm belongs to Candidatus Dirofilaria hongkongensis. It is likely that some infections previously reported as D. repens based on histological examination may have actually been due to Candidatus D. hongkongensis.

Hidden in the highlands: the description and phylogenetic position of a novel endemic freshwater crab species (Potamonautidae : Potamonautes) from Zimbabwe

2013 ◽  
Vol 27 (5) ◽  
pp. 530 ◽  
Author(s):  
Ethel E. Phiri ◽  
Savel R. Daniels
Keyword(s):  
Southern Africa ◽  
Sequence Data ◽  
Phylogenetic Position ◽  
12S Rrna ◽  
Mountain Range ◽  
Freshwater Crab ◽  
Crab Species ◽  
Mountainous Regions ◽  
Freshwater Crabs ◽  
Mtdna Sequence

A recent sampling endeavour of freshwater crabs along the high-lying streams of the Nyanga mountain range in Mutare (Eastern Highlands, Zimbabwe) yielded a morphologically distinct, as yet undescribed species. The novel Zimbabwean species is compared to the 16 described species from southern Africa based on mtDNA sequence data derived from three partial gene sequences (12S rRNA, 16S rRNA and COI). The new Zimbabwean species was found to be a sister taxon to Potamonautes mulanjeensis. These two species are morphologically and genetically easily differentiated. The new species is described as Potamonautes mutareensis, sp. nov. and is compared morphologically to the known freshwater crab species of southern Africa. A dichotomous key to the four described freshwater crab species that occur in Zimbabwe is also provided. Our results suggest that species diversity and endemism of freshwater decapods and other habitat specialists is likely to be high in unsampled mountainous regions.

Characterisation of peacock (Pavo cristatus) mitochondrial 12S rRNA sequence and its use in differentiation from closely related poultry species

2007 ◽  
Vol 48 (2) ◽  
pp. 162-166 ◽  
Author(s):  
M. Saini ◽  
D.K. Das ◽  
A. Dhara ◽  
D. Swarup ◽  
M.P. Yadav ◽  
...  
Keyword(s):  
12S Rrna ◽  
Rrna Sequence ◽  
Pavo Cristatus ◽  
Mitochondrial 12S Rrna

Case Report: An Eyelid Nodule Caused by Candidatus Dirofilaria hongkongensis Diagnosed by Mitochondrial 12S rRNA Sequence

2022 ◽  
Vol 106 (1) ◽  
pp. 199-203
Author(s):  
Suppapong Tirakunwichcha ◽  
Lalana Sansopha ◽  
Chaturong Putaporntip ◽  
Somchai Jongwutiwes
Keyword(s):  
Case Report ◽  
Histological Examination ◽  
External Surface ◽  
Histopathological Examination ◽  
Dirofilaria Repens ◽  
12S Rrna ◽  
Rrna Sequence ◽  
Immature Female ◽  
Upper Eyelid ◽  
Mitochondrial 12S Rrna

ABSTRACT. A 59-year-old female living in Rayong Province, eastern Thailand, presented with painless, right upper eyelid nodule for 3 months. Upon removal of the eyelid mass, a well-circumscribed, firm globular mass with diameter about 1 cm was found. Histopathological examination revealed an immature female dirofilarial worm reminiscent of Dirofilaria repens, characterized by prominent sharp longitudinal ridges at external surface of the cuticle. Analysis of the mitochondrial 12S rRNA sequence showed that the worm belongs to Candidatus Dirofilaria hongkongensis. It is likely that some infections previously reported as D. repens based on histological examination may have actually been due to Candidatus D. hongkongensis.

The phylogeography of Asian Schistosoma (Trematoda: Schistosomatidae)

Parasitology ◽  
2002 ◽  
Vol 125 (2) ◽  
pp. 99-112 ◽  
Author(s):  
S. W. ATTWOOD ◽  
E. S. UPATHAM ◽  
X. H. MENG ◽  
D.-C. QIU ◽  
V. R. SOUTHGATE
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Rrna Genes ◽  
12S Rrna ◽  
28S Rrna ◽  
Asian Origin ◽  
Dna Sequence Data ◽  
African Group ◽  
Mitochondrial 12S Rrna ◽  
Central Thailand

Partial (DNA) sequences are presented for 2 nuclear (18S and 28S rRNA genes) and 2 mitochondrial (12S rRNA and ND1 genes) loci for 5 species belonging to the Schistosoma japonicum, S. sinensium and S. indicum groups of Asian Schistosoma. Fresh field isolates were collected and cultured for the following taxa: S. incognitum (S. indicum group, central Thailand), S. mekongi (S. japonicum group, southern Laos), S. ovuncatum (S. sinensium group, northern Thailand), S. spindale (S. indicum group, northeast Thailand and central Thailand isolates) and S. sinensium (S. sinensium group, Sichuan Province, China). This represents the first published DNA sequence data for S. ovuncatum and for S. sinensiums.s. from the type locality in China. The paper also presents the first sequence data at the above loci for S. incognitum (except for the 28S sequences) and S. sinensium. Congruence was observed between the phylogenies estimated for each locus, although the relationships of S. incognitum were not so well resolved. Fitch–Margoliash, maximum likelihood (ML) and maximum parsimony methods were used to estimate the phylogenies and the agreement between them was similar to that observed between loci. The ML tree was considered to best represent the data and additional 28S sequences (taken from the GenBank), for S. haematobium, S. japonicum, S. mansoni and Orientobilharzia turkestanicum, were used to construct an overall phylogeny. The S. indicum group taxa showed considerable divergence from the other Asian species and closest affinity with the African group. S. ovuncatum and S. sinensium appeared as sister taxa but their status as sibling species remained supported. The findings are discussed in the context of phylogeographical hypotheses for the origin of Schistosoma. An Asian origin for Schistosoma is also considered.

scholarly journals Development of mitochondrial 12S rRNA gene for identification of dog and rat in beef using multiplex PCR

2019 ◽  
Vol 44 (1) ◽  
pp. 10 ◽  
Author(s):  
M. Cahyadi ◽  
I. M. Taufik ◽  
A. Pramono ◽  
Z. H. Abdurrahman
Keyword(s):  
Multiplex Pcr ◽  
Sequence Data ◽  
Uv Light ◽  
Reference Sequence ◽  
12S Rrna ◽  
Rrna Gene ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

The 12S rRNA gene is one of unique regions in mitochondrial genome usually used for phylogenetic studies and species identification. The objective of present study was to develop species specific primers from mitochondrial 12S rRNA gene for identification of dog and rat in beef by using multiplex PCR assay. Three primer pairs of mitochondrial 12S rRNA gene specific for bovine, dog and rat were designed and selected to evaluate their specificity and fidelity. Moreover, a total of twelve DNA samples extracted from meat tissue were also prepared to test those primers using simplex and multiplex PCR. The PCR products were then visualized using 2% of agarose gel under the UV light and three of them were sequenced. In addition, sequence data were analyzed using Clustal Omega software and BLAST. The result showed that simplex PCR assay successfully amplified DNA targets which are respectively indicated by 155 bp (bovine), 244 bp (dog), and 491 bp (rat) of DNA bands. Furthermore, DNA sample sequences were identically similar to reference sequence used in this study. Multiplex and simplex PCR analyses also indicated that these primer pairs specifically amplified DNA target for each species in the samples containing various species. The results suggested that designed primers in this study could be used to identify dog and rat in raw beef containing these species meat. Further experiment should be conducted using meat-processed products and commercial meat products as samples.

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