High-Level Expression of Biologically Active Human Prolactin from Recombinant Baculovirus in Insect Cells

2000 ◽  
Vol 20 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Tapas Das ◽  
Paul W. Johns ◽  
Vincent Goffin ◽  
Paul Kelly ◽  
Bruce Kelder ◽  
...  
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Biologically Active ◽  
High Level Expression ◽  
Human Prolactin ◽  
High Level

A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells

2009 ◽  
Vol 31 (5) ◽  
pp. 623-627 ◽  
Author(s):  
Takuya Shishido ◽  
Naoya Kurata ◽  
Myung Eui Yoon ◽  
Tsutomu Tanaka ◽  
Hideki Yamaji ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Expression Vector ◽  
Insect Cells ◽  
Selectable Marker ◽  
Continuous Production ◽  
High Level Expression ◽  
High Level

High level production of potyvirus-like particles in insect cells infected with recombinant baculovirus

1994 ◽  
Vol 136 (3-4) ◽  
pp. 375-380 ◽  
Author(s):  
S. J. Edwards ◽  
M. B. Hayden ◽  
R. C. Hamilton ◽  
J. A. Haynes ◽  
I. T. Nisbet ◽  
...  
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
High Level ◽  
Level Production

High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells

Gene ◽  
1993 ◽  
Vol 128 (2) ◽  
pp. 307-308 ◽  
Author(s):  
Bernard Y. Amegadzie ◽  
Deborah Jiampetti ◽  
Robert J. Craig ◽  
Edward Appelbaum ◽  
Allan R. Shatzman ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Insect Cells ◽  
Cytosolic Phospholipase A2 ◽  
Biologically Active ◽  
Cytosolic Phospholipase ◽  
Infected Insect ◽  
High Level ◽  
Level Production

High Level Expression and Secretion of Biologically Active Leech Hirudin Variant 1 (HV1) by Escherichia coli

1992 ◽  
pp. 62-64 ◽  
Author(s):  
Satoru Misawa ◽  
Hideyuki Furuya ◽  
Hitoshi Matsuda ◽  
Shin-Ichiro Abe ◽  
Hideya Hayashi
Keyword(s):  
Escherichia Coli ◽  
Biologically Active ◽  
High Level Expression ◽  
High Level

High-Level Expression and Purification of Biologically Active Recombinant Pokeweed Antiviral Protein

1999 ◽  
Vol 16 (2) ◽  
pp. 359-368 ◽  
Author(s):  
Francis Rajamohan ◽  
Cherri R. Engstrom ◽  
Tammy J. Denton ◽  
Lisa A. Engen ◽  
Igor Kourinov ◽  
...  
Keyword(s):  
Biologically Active ◽  
Pokeweed Antiviral Protein ◽  
Expression And Purification ◽  
Antiviral Protein ◽  
High Level Expression ◽  
High Level

scholarly journals Structural and functional analysis of a promoter of the human granulin/epithelin gene

1996 ◽  
Vol 319 (2) ◽  
pp. 441-447 ◽  
Author(s):  
Vijay BHANDARI ◽  
Rachael DANIEL ◽  
Pheng Siew LIM ◽  
Andrew BATEMAN
Keyword(s):  
Promoter Activity ◽  
Biologically Active ◽  
Gene Products ◽  
High Level Expression ◽  
Haematopoietic Cells ◽  
Molecular Masses ◽  
Chloramphenicol Acetyltransferase Gene ◽  
High Level

Granulins (grns) or epithelins (epis) are peptides with molecular masses of approx. 6 kDa that modulate the growth of cells. The precursor for the grns/epis, which might itself be biologically active, is a secreted glycoprotein containing multiple repeats of the grn/epi motif. Grn/epi mRNA occurs widely in vivo, particularly in tissues rich in epithelial and haematopoietic cells. To understand better the role of the gene products for grn/epi it is important to determine the patterns of grn/epi gene expression and how this is regulated. To assist in this we have obtained the 5´ sequence of the human grn/epi gene, and using chimaeras of the grn/epi -5´ sequence and the chloramphenicol acetyltransferase gene we have shown a strong promoter activity associated with the 5´ sequence of the human grn/epi gene. We have further delineated regions of the 5´ sequence that confer high-level expression on the chimaeric gene.

Use of the silkworm, Bombyx mori, and an insect baculovirus vector for high-level expression and secretion of biologically active mouse interleukin-3

Gene ◽  
1987 ◽  
Vol 58 (2-3) ◽  
pp. 273-281 ◽  
Author(s):  
Atsushi Miyajima ◽  
Jolanda Schreurs ◽  
Keiko Otsu ◽  
Atsushi Kondo ◽  
Ken-ichi Aral ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Biologically Active ◽  
High Level Expression ◽  
Baculovirus Vector ◽  
Interleukin 3 ◽  
High Level ◽  
Silkworm Bombyx Mori
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