High level production of potyvirus-like particles in insect cells infected with recombinant baculovirus

1994 ◽  
Vol 136 (3-4) ◽  
pp. 375-380 ◽  
Author(s):  
S. J. Edwards ◽  
M. B. Hayden ◽  
R. C. Hamilton ◽  
J. A. Haynes ◽  
I. T. Nisbet ◽  
...  
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
High Level ◽  
Level Production

High-Level Expression of Biologically Active Human Prolactin from Recombinant Baculovirus in Insect Cells

2000 ◽  
Vol 20 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Tapas Das ◽  
Paul W. Johns ◽  
Vincent Goffin ◽  
Paul Kelly ◽  
Bruce Kelder ◽  
...  
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Biologically Active ◽  
High Level Expression ◽  
Human Prolactin ◽  
High Level

High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells

Gene ◽  
1993 ◽  
Vol 128 (2) ◽  
pp. 307-308 ◽  
Author(s):  
Bernard Y. Amegadzie ◽  
Deborah Jiampetti ◽  
Robert J. Craig ◽  
Edward Appelbaum ◽  
Allan R. Shatzman ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Insect Cells ◽  
Cytosolic Phospholipase A2 ◽  
Biologically Active ◽  
Cytosolic Phospholipase ◽  
Infected Insect ◽  
High Level ◽  
Level Production

High-level production of human parathyroid hormone in Bombyx mori larvae and BmN cells using recombinant baculovirus

Gene ◽  
1995 ◽  
Vol 167 (1-2) ◽  
pp. 33-39 ◽  
Author(s):  
Sinnakaruppan Mathavan ◽  
Vigdis T. Gautvik ◽  
Erik Rokkones ◽  
Ole Kristoffer Olstad ◽  
B. Najma Kareem ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bombyx Mori ◽  
Recombinant Baculovirus ◽  
Human Parathyroid Hormone ◽  
High Level ◽  
Level Production ◽  
Bmn Cells

scholarly journals High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity

2003 ◽  
Vol 10 (4) ◽  
pp. 596-601 ◽  
Author(s):  
Shinya Fukumoto ◽  
Xuenan Xuan ◽  
Kimie Kadota ◽  
Ikuo Igarashi ◽  
Chihiro Sugimoto ◽  
...  
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Babesia Gibsoni ◽  
Gene Encoding ◽  
Hydrophobic Region ◽  
Immunodominant Antigen ◽  
Diagnostic Potential ◽  
Highly Hydrophobic ◽  
High Level

ABSTRACT Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids [aa]) was expressed in insect cells by a recombinant baculovirus. The highly hydrophobic C-terminal 20-aa regions seems to be a transmembrane region, which was evidenced by the fact that rP50t was effectively secreted into the supernatant of insect cells infected with the recombinant baculovirus. N-terminal amino acid sequence analysis of rP50t indicated that N-terminal 19 aa function as a signal peptide. The expression level of rP50t reached up to 2 mg per 108 cells infected with the recombinant baculovirus. The immunogenic property of rP50t was evaluated by an immunization test in mice. Mice immunized with rP50t induced a high-level antibody titer against the B. gibsoni merozoite. Monoclonal antibodies (MAbs) to rP50t were produced in mice to determine the immunogenic regions of P50. The epitope(s) recognized by all five MAbs were located between aa 190 and 273, suggesting that the central part of P50 is a highly immunogenic region. The diagnostic potential of rP50t was evaluated using an enzyme-linked immunosorbent assay (ELISA). The ELISA was able to differentiate clearly (P < 0.0001) between B. gibsoni-infected dog serum and B. canis-infected dog serum or noninfected dog serum. Our results indicated that the rP50t may provide a useful potential immunogenic reagent for use in diagnosis and as a subunit vaccine to control B. gibsoni infection in dogs.

High-level production of human α- and β-globins in insect cells

1992 ◽  
Vol 3 (2) ◽  
pp. 134-141 ◽  
Author(s):  
Duncan R. Groebe ◽  
Mark R. Busch ◽  
Twee Yon Moy Tsao ◽  
Frederick Y. Luh ◽  
Ming F. Tam ◽  
...  
Keyword(s):  
Insect Cells ◽  
High Level ◽  
Level Production
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