scholarly journals Complementation of Virus Movement in Transgenic Tobacco Expressing the Cucumber Mosaic Virus 3a Gene

Virology ◽  
1995 ◽  
Vol 209 (1) ◽  
pp. 188-199 ◽  
Author(s):  
Igor B. Kaplan ◽  
Michael H. Shintaku ◽  
Qiubo Li ◽  
Lee Zhang ◽  
Loren E. Marsh ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Virus Movement

scholarly journals Cucumber mosaic virus 2a polymerase and 3a movement proteins independently affect both virus movement and the timing of symptom development in zucchini squash

2005 ◽  
Vol 86 (4) ◽  
pp. 1213-1222 ◽  
Author(s):  
Seung Kook Choi ◽  
Peter Palukaitis ◽  
Byoung Eun Min ◽  
Mi Yeon Lee ◽  
Jang Kyung Choi ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Cell Movement ◽  
Virus Movement ◽  
Differential Rate ◽  
Systemic Symptom ◽  
Symptom Development ◽  
Movement Proteins ◽  
The Difference ◽  
Systemic Symptoms

The basis for differences in the timing of systemic symptom elicitation in zucchini squash between a pepper strain of Cucumber mosaic virus (Pf-CMV) and a cucurbit strain (Fny-CMV) was analysed. The difference in timing of appearance of systemic symptoms was shown to map to both RNA 2 and RNA 3 of Pf-CMV, with pseudorecombinant viruses containing either RNA 2 or RNA 3 from Pf-CMV showing an intermediate rate of systemic symptom development compared with those containing both or neither Pf-CMV RNAs. Symptom phenotype was shown to map to two single-nucleotide changes, both in codons for Ile at aa 267 and 168 (in Fny-CMV RNAs 2 and 3, respectively) to Thr (in Pf-CMV RNAs 2 and 3). The differential rate of symptom development was shown to be due to differences in the rates of cell-to-cell movement in the inoculated cotyledons, as well as differences in the rate of egress of the virus from the inoculated leaves. These data indicate that both the CMV 3a movement protein and the CMV 2a polymerase protein affect the rate of movement of CMV in zucchini squash and that these two proteins function independently of each other in their interactions with the host, facilitating virus movement.

Viral Protection in Transgenic Tobacco Plants Expressing the Cucumber Mosaic Virus Coat Protein or its Antisense RNA

1988 ◽  
Vol 6 (5) ◽  
pp. 549-557 ◽  
Author(s):  
Maria Cuozzo ◽  
Keith M. O'Connell ◽  
Wojciech Kaniewski ◽  
Rong-Xiang Fang ◽  
Nam-Hai Chua ◽  
...  
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Antisense Rna ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals The C-terminal 33 amino acids of the cucumber mosaic virus 3a protein affect virus movement, RNA binding and inhibition of infection and translation

2004 ◽  
Vol 85 (1) ◽  
pp. 221-230 ◽  
Author(s):  
Sang Hyon Kim ◽  
Natalia O. Kalinina ◽  
Igor Andreev ◽  
Eugene V. Ryabov ◽  
Alexander G. Fitzgerald ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Binding ◽  
Virus Movement

Effectiveness of coat protein and defective replicase gene-mediated resistance against Australian isolates of cucumber mosaic virus

1998 ◽  
Vol 38 (4) ◽  
pp. 375 ◽  
Author(s):  
Z. Singh ◽  
M. G. K. Jones ◽  
R. A. C. Jones
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Extreme Resistance ◽  
Tobacco Plants ◽  
Systemic Movement ◽  
Cp Gene ◽  
Subgroup Ii

Summary. Transgenic tobacco (Nicotiana tabacum) plants of (i) cv. Samsun NN containing the cauliflower mosaic virus 35S constitutive promoter linked to a defective replicase (DR) gene derived from cucumber mosaic virus (CMV) subgroup I isolate Fny, and (ii) cv. Xanthi containing the CaMV 35S promoter linked to the coat protein (CP) gene of CMV subgroup I isolate C were tested for resistance to various Australian isolates of CMV. The tobacco plants were challenged with 3 CMV subgroup 1 isolates (BNRR, BMR and B6) using sap inoculation. When used to challenge non-transgenic tobacco plants with 5 subgroup II CMV isolates from lupins (LY, LCH, LAcc, LGu and LD), this inoculation method did not result in systemic infection so graft inoculation was used instead to challenge transgenic plants with these 5 isolates. When plants of the line with the DR gene were challenged with the 3 subgroup I isolates, extreme resistance was revealed as none showed symptoms and CMV was not detectable by ELISA. When the same 3 isolates were inoculated to the 3 lines with the CP gene, resistance was characterised by fewer plants becoming virus infected, delayed systemic movement and, in the plants that were infected, partial remission of symptoms plus somewhat decreased virus concentration. Challenge of transgenic plants with DR or CP with the 5 subgroup II isolates resulted in fewer plants becoming infected. Actual numbers of plants infected varied with line and subgroup II isolate and the DR gene was as effective as the CP gene at decreasing infection. With subgroup II isolate LY, infection was associated with remission of symptoms and with the other 4 isolates with delayed systemic movement. Thus the DR gene approach was more effective than the CP approach in obtaining extreme resistance against Australian subgroup I isolates of CMV. These results suggest that introducing a similar DR gene construct made from a subgroup II isolate from lupins into commercial lupin cultivars may be a suitable strategy for obtaining extreme resistance to subgroup II isolates from lupins.

Sign in / Sign up

Export Citation Format

Share Document