scholarly journals Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor

Vaccines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1003
Author(s):  
Xia Luo ◽  
Yinjie Niu ◽  
Xiaozhe Fu ◽  
Qiang Lin ◽  
Hongru Liang ◽  
...  

Mandarin fish (Siniperca chuatsi) is one of the important cultured fish species in China. Infectious spleen and kidney necrosis virus (ISKNV) and Siniperca Chuatsi rhabdovirus (SCRV) have hindered the development of mandarin fish farming industry. Vaccination is the most effective method for control of viral diseases, however viral vaccine production requires the large-scale culture of cells. Herein, a suspension culture system of Chinese perch brain cell (CPB) was developed on Cytodex 1 microcarrier in a stirred bioreactor. Firstly, CPB cells were cultured using Cytodex 1 microcarrier in 125 mL stirring flasks. With the optimum operational parameters, CPB cells grew well, distributed uniformly, and could fully cover the microcarriers. Then, CPB cells were digested with trypsin and expanded step-by-step with different expansion ratios from the 125 mL stirring bottle to a 500 mL stirring bottle, and finally to a 3-L bioreactor. Results showed that with an expansion ratio of 1:3, we achieved a high cell density level (2.25 × 106 cells/mL) with an efficient use of the microcarriers, which also confirmed the data obtained from the 125 mL stirring flask. Moreover, obvious cytopathic effects (CPE) were observed in the suspended CPB cells post-infection with ISKNV and SCRV. This study provided a large-scale culture system of CPB cells for virus vaccine production.

2013 ◽  
Vol 694-697 ◽  
pp. 148-153 ◽  
Author(s):  
Li Kuan Zhu ◽  
Bo Yan Song ◽  
Zhen Long Wang ◽  
Yu Kui Wang

This paper mainly makes comparative analysis on four main types of blade in stirred bioreactor by Computational Fluid Dynamics(CFD) simulation. Firstly we establish simulation method suited for stirred bioreactor, then simulate the velocity and shear force of flow field in the bioreactor. No matter from flow field mixing or shear force aspect, Elephant Ear blades is the most suitable for cell large scale culture. At last, it optimizes the installation method and angle of Elephant Ear blades. It concludes that anticlockwise rotation and 45°installation angle is the optimum.


1992 ◽  
Vol 66 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Y Nakamura ◽  
Y Tokuda ◽  
M Iwasawa ◽  
H Tsukamoto ◽  
M Kidokoro ◽  
...  

2010 ◽  
Vol 13 (2) ◽  
pp. 151-162 ◽  
Author(s):  
Guoqiang Zhang ◽  
Wuying Chu ◽  
Songnian Hu ◽  
Tao Meng ◽  
Linlin Pan ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document