Introduction and History of Foot-and-Mouth Disease Virus

Author(s):  
B. W. J. Mahy
PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49650 ◽  
Author(s):  
Begoña Valdazo-González ◽  
Lilyana Polihronova ◽  
Tsviatko Alexandrov ◽  
Preben Normann ◽  
Nick J. Knowles ◽  
...  

2018 ◽  
Vol 67 (1) ◽  
pp. 33
Author(s):  
Ö. B. INCE ◽  
R. KALKAN ◽  
S. ÇAKIR

The study was conducted using two ELISA methods - the liquid phase blocking ELISA (LPBE) and solid phase competition ELISA (SPCE) for the detection of foot-and-mouth disease virus (FMDV) serotype A- and O-specific antibodies of different cattle breeds in Turkey. These methods were compared in 426 cattle previously vaccinated with oil-adjuvanted bivalent vaccine as well as in sera from 40 cattle with no history of foot-and-mouth disease infection or vaccination. The results were found that SPCE had a better specificity (serotype A; 100% and serotype O; 97.50%) than LPBE (serotype A 95.00% and serotype O 92.50%). Sensitivity of SPCE had also better values (serotype A; 99.30% and serotype O; 98.59%) than LPBE (serotype A; 97.89% and serotype O; 96.48%). The results of the present study showed that the SPCE method is more reliable than LPBE.


2014 ◽  
Vol 63 (1) ◽  
pp. e27-e38 ◽  
Author(s):  
A. Ludi ◽  
Z. Ahmed ◽  
L. W. Pomeroy ◽  
S. J. Pauszek ◽  
G. R. Smoliga ◽  
...  

Author(s):  
S. S. Breese ◽  
H. L. Bachrach

Models for the structure of foot-and-mouth disease virus (FMDV) have been proposed from chemical and physical measurements (Brown, et al., 1970; Talbot and Brown, 1972; Strohmaier and Adam, 1976) and from rotational image-enhancement electron microscopy (Breese, et al., 1965). In this report we examine the surface structure of FMDV particles by high resolution electron microscopy and compare it with that of particles in which the outermost capsid protein VP3 (ca. 30, 000 daltons) has been split into smaller segments, two of which VP3a and VP3b have molecular weights of about 15, 000 daltons (Bachrach, et al., 1975).Highly purified and concentrated type A12, strain 119 FMDV (5 mg/ml) was prepared as previously described (Bachrach, et al., 1964) and stored at 4°C in 0. 2 M KC1-0. 5 M potassium phosphate buffer at pH 7. 5. For electron microscopy, 1. 0 ml samples of purified virus and trypsin-treated virus were dialyzed at 4°C against 0. 2 M NH4OAC at pH 7. 3, deposited onto carbonized formvar-coated copper screens and stained with phosphotungstic acid, pH 7. 3.


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