Structural and Functional Modulation of Ion Channels by Specific Lipids: from Model Systems to Cell Membranes

2006 ◽  
pp. 203-231
Author(s):  
Asia M. Fernández ◽  
José A. Poveda ◽  
José A. Encinar ◽  
Andrés Morales ◽  
José M. González-Ros
Keyword(s):  
Ion Channels ◽  
Cell Membranes ◽  
Model Systems ◽  
Specific Lipids ◽  
Functional Modulation

scholarly journals A complicated complex: Ion channels, voltage sensing, cell membranes and peptide inhibitors

2018 ◽  
Vol 679 ◽  
pp. 35-47 ◽  
Author(s):  
Alan H. Zhang ◽  
Gagan Sharma ◽  
Eivind A.B. Undheim ◽  
Xinying Jia ◽  
Mehdi Mobli
Keyword(s):  
Ion Channels ◽  
Cell Membranes ◽  
Peptide Inhibitors ◽  
Voltage Sensing ◽  
Complex Ion

scholarly journals Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors

2020 ◽  
Vol 10 (6) ◽  
pp. 344 ◽  
Author(s):  
Clemens L. Schoepf ◽  
Maximilian Zeidler ◽  
Lisa Spiecker ◽  
Georg Kern ◽  
Judith Lechner ◽  
...  
Keyword(s):  
Ion Channels ◽  
Functional Characterization ◽  
Scientific Progress ◽  
Model Systems ◽  
Patient Specific ◽  
Preclinical Research ◽  
Specific Research ◽  
Voltage Gated ◽  
Trpv1 Channels ◽  
Induced Pluripotent

Preclinical research using different rodent model systems has largely contributed to the scientific progress in the pain field, however, it suffers from interspecies differences, limited access to human models, and ethical concerns. Human induced pluripotent stem cells (iPSCs) offer major advantages over animal models, i.e., they retain the genome of the donor (patient), and thus allow donor-specific and cell-type specific research. Consequently, human iPSC-derived nociceptors (iDNs) offer intriguingly new possibilities for patient-specific, animal-free research. In the present study, we characterized iDNs based on the expression of well described nociceptive markers and ion channels, and we conducted a side-by-side comparison of iDNs with mouse sensory neurons. Specifically, immunofluorescence (IF) analyses with selected markers including early somatosensory transcription factors (BRN3A/ISL1/RUNX1), the low-affinity nerve growth factor receptor (p75), hyperpolarization-activated cyclic nucleotide-gated channels (HCN), as well as high voltage-gated calcium channels (VGCC) of the CaV2 type, calcium permeable TRPV1 channels, and ionotropic GABAA receptors, were used to address the characteristics of the iDN phenotype. We further combined IF analyses with microfluorimetric Ca2+ measurements to address the functionality of these ion channels in iDNs. Thus, we provide a detailed morphological and functional characterization of iDNs, thereby, underpinning their enormous potential as an animal-free alternative for human specific research in the pain field for unveiling pathophysiological mechanisms and for unbiased, disease-specific personalized drug development.

scholarly journals Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Anastasiia A. Zakharova ◽  
Svetlana S. Efimova ◽  
Valery V. Malev ◽  
Olga S. Ostroumova
Keyword(s):  
Ion Channels ◽  
Lipid Bilayers ◽  
Cell Membranes ◽  
Membrane Lipids ◽  
Electrical Potential ◽  
Membrane Conductance ◽  
Pressure Profile ◽  
Molecular Shape ◽  
Bathing Solution ◽  
Fungal Cell

Abstract The one-sided addition of fengycin (FE) to planar lipid bilayers mimicking target fungal cell membranes up to 0.1 to 0.5 μM in the membrane bathing solution leads to the formation of well-defined and well-reproducible single-ion channels of various conductances in the picosiemens range. FE channels were characterized by asymmetric conductance-voltage characteristic. Membranes treated with FE showed nonideal cationic selectivity in potassium chloride bathing solutions. The membrane conductance induced by FE increased with the second power of the lipopeptide aqueous concentration, suggesting that at least FE dimers are involved in the formation of conductive subunits. The pore formation ability of FE was not distinctly affected by the molecular shape of membrane lipids but strongly depended on the presence of negatively charged species in the bilayer. FE channels were characterized by weakly pronounced voltage gating. Small molecules known to modify the transmembrane distribution of electrical potential and the lateral pressure profile were used to modulate the channel-forming activity of FE. The observed effects of membrane modifiers were attributed to changes in lipid packing and lipopeptide oligomerization in the membrane.

Sign in / Sign up

Export Citation Format

Share Document