Yeast Biodiversity and Culture Collections

2006 ◽  
pp. 31-44 ◽  
Author(s):  
Vincent Robert ◽  
Joost Stalpers ◽  
Teun Boekhout ◽  
Shu-hui Tan
Keyword(s):  
Culture Collections

In Vitro Establishment of Powdery Mildew (Microsphaera pulchra) on Microshoots of Flowering Dogwood

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506a-506
Author(s):  
L.A. Klein ◽  
M.T. Windham ◽  
R.N. Trigiano
Keyword(s):  
Powdery Mildew ◽  
Woody Plant ◽  
Infected Individual ◽  
Callus Cultures ◽  
Fungal Culture ◽  
Culture Collections ◽  
Flowering Dogwood ◽  
Plant Parasite ◽  
In Vitro Establishment

Microshoot and callus cultures of Cornus florida (flowering dogwood), which were grown on woody plant medium amended with BA, were inoculated with Microsphaera pulchra (an obligate plant parasite) by gently shaking infected leaves bearing numerous conidia over the tissue. Culture dishes were sealed with parafilm and incubated at 24 °C with 25 mol·m–2·s–1 provided by cool fluorescent bulbs for 15 h. Cultures were examined with a dissecting scope every 24 h and cultures transferred when contaminating fungi were present. Specimens were prepared light microscopy and SEM. The fungus infected individual callus cells, but did not sporulate. In contrast, powdery mildew was well-established (both primary and secondary hyphae) in 70% of the microshoot cultures after 6 days and sporulated on 20% by 7 to 8 days. The cellular relationship between host and pathogen in vitro was similar to that found in greenhouse-grown plants. This technique has possible applications in maintaining fungal culture collections and studying host–pathogen relationships under more stringently controlled conditions.

scholarly journals Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
K. Böhme ◽  
P. Cremonesi ◽  
M. Severgnini ◽  
Tomás G. Villa ◽  
I. C. Fernández-No ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Cost Effective ◽  
Rrna Gene ◽  
Bacterial Identification ◽  
Dot Blot ◽  
Culture Collections ◽  
Ligation Detection Reaction ◽  
Flow Through ◽  
Food Safety And Quality

Traditional culturing methods are still commonly applied for bacterial identification in the food control sector, despite being time and labor intensive. Microarray technologies represent an interesting alternative. However, they require higher costs and technical expertise, making them still inappropriate for microbial routine analysis. The present study describes the development of an efficient method for bacterial identification based on flow-through reverse dot-blot (FT-RDB) hybridization on membranes, coupled to the high specific ligation detection reaction (LDR). First, the methodology was optimized by testing different types of ligase enzymes, labeling, and membranes. Furthermore, specific oligonucleotide probes were designed based on the 16S rRNA gene, using the bioinformatic tool Oligonucleotide Retrieving for Molecular Applications (ORMA). Four probes were selected and synthesized, being specific forAeromonasspp.,Pseudomonasspp.,Shewanellaspp., andMorganella morganii, respectively. For the validation of the probes, 16 reference strains from type culture collections were tested by LDR and FT-RDB hybridization using universal arrays spotted onto membranes. In conclusion, the described methodology could be applied for the rapid, accurate, and cost-effective identification of bacterial species, exhibiting special relevance in food safety and quality.

scholarly journals List of new names and new combinations previously effectively, but not validly, published

2006 ◽  
Vol 56 (9) ◽  
pp. 2025-2027 ◽  
Keyword(s):  
New Species ◽  
The Other ◽  
New Combination ◽  
Editorial Office ◽  
New Combinations ◽  
Original Publication ◽  
Culture Collections ◽  
New Subspecies ◽  
Type Strains

The purpose of this announcement is to effect the valid publication of the following new names and new combinations under the procedure described in the Bacteriological Code (1990 Revision). Authors and other individuals wishing to have new names and/or combinations included in future lists should send three copies of the pertinent reprint or photocopies thereof to the IJSEM Editorial Office for confirmation that all of the other requirements for valid publication have been met. It is also a requirement of IJSEM and the ICSP that authors of new species, new subspecies and new combinations provide evidence that types are deposited in two recognized culture collections in two different countries (i.e. documents certifying deposition and availability of type strains). It should be noted that the date of valid publication of these new names and combinations is the date of publication of this list, not the date of the original publication of the names and combinations. The authors of the new names and combinations are as given below, and these authors' names will be included in the author index of the present issue and in the volume author index. Inclusion of a name on these lists validates the publication of the name and thereby makes it available in bacteriological nomenclature. The inclusion of a name on this list is not to be construed as taxonomic acceptance of the taxon to which the name is applied. Indeed, some of these names may, in time, be shown to be synonyms, or the organisms may be transferred to another genus, thus necessitating the creation of a new combination.

scholarly journals List of new names and new combinations previously effectively, but not validly, published

2010 ◽  
Vol 60 (9) ◽  
pp. 1985-1986 ◽  
Keyword(s):  
New Species ◽  
The Other ◽  
New Combination ◽  
Editorial Office ◽  
New Combinations ◽  
Original Publication ◽  
Culture Collections ◽  
Electronic Copy ◽  
Published Paper

The purpose of this announcement is to effect the valid publication of the following effectively published new names and new combinations under the procedure described in the Bacteriological Code (1990 Revision). Authors and other individuals wishing to have new names and/or combinations included in future lists should send three copies of the pertinent reprint or photocopies thereof, or an electronic copy of the published paper, to the IJSEM Editorial Office for confirmation that all of the other requirements for valid publication have been met. It is also a requirement of IJSEM and the ICSP that authors of new species, new subspecies and new combinations provide evidence that types are deposited in two recognized culture collections in two different countries. It should be noted that the date of valid publication of these new names and combinations is the date of publication of this list, not the date of the original publication of the names and combinations. The authors of the new names and combinations are as given below, and these authors’ names will be included in the author index of the present issue. Inclusion of a name on these lists validates the publication of the name and thereby makes it available in bacteriological nomenclature. The inclusion of a name on this list is not to be construed as taxonomic acceptance of the taxon to which the name is applied. Indeed, some of these names may, in time, be shown to be synonyms, or the organisms may be transferred to another genus, thus necessitating the creation of a new combination.

scholarly journals List of new names and new combinations previously effectively, but not validly, published

2007 ◽  
Vol 57 (5) ◽  
pp. 893-897 ◽  
Keyword(s):  
The Other ◽  
New Combination ◽  
Editorial Office ◽  
New Combinations ◽  
Original Publication ◽  
Culture Collections ◽  
Electronic Copy ◽  
Published Paper ◽  
Type Strains

The purpose of this announcement is to effect the valid publication of the following new names and new combinations under the procedure described in the Bacteriological Code (1990 Revision). Authors and other individuals wishing to have new names and/or combinations included in future lists should send three copies of the pertinent reprint or photocopies thereof, or an electronic copy of the published paper, to the IJSEM Editorial Office for confirmation that all of the other requirements for valid publication have been met. It is also a requirement of IJSEM and the ICSP that authors of new species, new subspecies and new combinations provide evidence that types are deposited in two recognized culture collections in two different countries (i.e. documents certifying deposition and availability of type strains). It should be noted that the date of valid publication of these new names and combinations is the date of publication of this list, not the date of the original publication of the names and combinations. The authors of the new names and combinations are as given below, and these authors' names will be included in the author index of the present issue and in the volume author index. Inclusion of a name on these lists validates the publication of the name and thereby makes it available in bacteriological nomenclature. The inclusion of a name on this list is not to be construed as taxonomic acceptance of the taxon to which the name is applied. Indeed, some of these names may, in time, be shown to be synonyms, or the organisms may be transferred to another genus, thus necessitating the creation of a new combination.

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