Electrospun Nanocomposites and Stem Cells in Cardiac Tissue Engineering

2011 ◽  
pp. 215-242 ◽  
Author(s):  
Jorge A. Genovese ◽  
Cristiano Spadaccio ◽  
Alberto Rainer ◽  
Elvio Covino
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering

Application potential of three-dimensional silk fibroin scaffold using mesenchymal stem cells for cardiac regeneration

2021 ◽  
pp. 088532822110185
Author(s):  
Yuksel Cetin ◽  
Merve G Sahin ◽  
Fatma N Kok
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Troponin I ◽  
Cardiac Tissue Engineering ◽  
Swelling Ratio ◽  
Differentiation Potential ◽  
Cardiomyogenic Differentiation

Cardiac tissue engineering focusing on biomaterial scaffolds incorporating cells from different sources has been explored to regenerate or repair damaged area as a lifesaving approach.The aim of this study was to evaluate the cardiomyocyte differentiation potential of human adipose mesenchymal stem cells (hAD-MSCs) as an alternative cell source on silk fibroin (SF) scaffolds for cardiac tissue engineering. The change in surface morphology of SF scaffolds depending on SF concentration (1–6%, w/v) and increase in their porosity upon application of unidirectional freezing were visualized by scanning electron microscopy (SEM). Swelling ratio was found to increase 2.4 fold when SF amount was decreased from 4% to 2%. To avoid excessive swelling, 4% SF scaffold with swelling ratio of 10% (w/w) was chosen for further studies.Biodegradation rate of SF scaffolds depended on enzymatic activity was found to be 75% weight loss of SF scaffolds at the day 14. The phenotype of hAD-MSCs and their multi-linage potential into chondrocytes, osteocytes, and adipocytes were shown by flow cytometry and immunohistochemical staining, respectively.The viability of hAD-MSCs on 3D SF scaffolds was determined as 90%, 118%, and 138% after 1, 7, and 14 days, respectively. The use of 3D SF scaffolds was associated with increased production of cardiomyogenic biomarkers: α-actinin, troponin I, connexin 43, and myosin heavy chain. The fabricated 3D SF scaffolds were proved to sustain hAD-MSCs proliferation and cardiomyogenic differentiation therefore, hAD-MSCs on 3D SF scaffolds may useful tool to regenerate or repair damaged area using cardiac tissue engineering techniques.

Stem Cells for Cardiac Tissue Engineering

2011 ◽  
pp. 95-114
Author(s):  
Jennifer L. Young ◽  
Karen L. Christman ◽  
Adam J. Engler
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering

scholarly journals Physiological aspects of cardiac tissue engineering

2012 ◽  
Vol 303 (2) ◽  
pp. H133-H143 ◽  
Author(s):  
Thomas Eschenhagen ◽  
Alexandra Eder ◽  
Ingra Vollert ◽  
Arne Hansen
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Myocyte ◽  
Cardiac Tissue ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Basic Research ◽  
Cardiac Tissue Engineering ◽  
Predictive Toxicology

Cardiac tissue engineering aims at repairing the diseased heart and developing cardiac tissues for basic research and predictive toxicology applications. Since the first description of engineered heart tissue 15 years ago, major development steps were directed toward these three goals. Technical innovations led to improved three-dimensional cardiac tissue structure and near physiological contractile force development. Automation and standardization allow medium throughput screening. Larger constructs composed of many small engineered heart tissues or stacked cell sheet tissues were tested for cardiac repair and were associated with functional improvements in rats. Whether these approaches can be simply transferred to larger animals or the human patients remains to be tested. The availability of an unrestricted human cardiac myocyte cell source from human embryonic stem cells or human-induced pluripotent stem cells is a major breakthrough. This review summarizes current tissue engineering techniques with their strengths and limitations and possible future applications.

scholarly journals Chitosan as Functional Biomaterial for Designing Delivery Systems in Cardiac Therapies

Gels ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 253
Author(s):  
Bhaumik Patel ◽  
Ravi Manne ◽  
Devang B. Patel ◽  
Shashank Gorityala ◽  
Arunkumar Palaniappan ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Tissue ◽  
Mechanical Stability ◽  
Cardiac Tissue Engineering ◽  
Delivery Systems ◽  
Pharmaceutical Industries

Cardiovascular diseases are a leading cause of mortality across the globe, and transplant surgeries are not always successful since it is not always possible to replace most of the damaged heart tissues, for example in myocardial infarction. Chitosan, a natural polysaccharide, is an important biomaterial for many biomedical and pharmaceutical industries. Based on the origin, degree of deacetylation, structure, and biological functions, chitosan has emerged for vital tissue engineering applications. Recent studies reported that chitosan coupled with innovative technologies helped to load or deliver drugs or stem cells to repair the damaged heart tissue not just in a myocardial infarction but even in other cardiac therapies. Herein, we outlined the latest advances in cardiac tissue engineering mediated by chitosan overcoming the barriers in cardiac diseases. We reviewed in vitro and in vivo data reported dealing with drug delivery systems, scaffolds, or carriers fabricated using chitosan for stem cell therapy essential in cardiac tissue engineering. This comprehensive review also summarizes the properties of chitosan as a biomaterial substrate having sufficient mechanical stability that can stimulate the native collagen fibril structure for differentiating pluripotent stem cells and mesenchymal stem cells into cardiomyocytes for cardiac tissue engineering.

Stem Cells in Cardiac Tissue Engineering

Stem Cells ◽  
2010 ◽  
pp. 611-635 ◽  
Author(s):  
Nicolas Christoforou ◽  
Shaun Lim ◽  
Kam W. Leong
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering

scholarly journals Trichostatin A Enhances Differentiation of Human Induced Pluripotent Stem Cells to Cardiogenic Cells for Cardiac Tissue Engineering

2013 ◽  
Vol 2 (9) ◽  
pp. 715-725 ◽  
Author(s):  
Shiang Y. Lim ◽  
Priyadharshini Sivakumaran ◽  
Duncan E. Crombie ◽  
Gregory J. Dusting ◽  
Alice Pébay ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cardiac Tissue ◽  
Trichostatin A ◽  
Cardiac Tissue Engineering ◽  
Induced Pluripotent

scholarly journals Direct Mechanical Stimulation of Stem Cells: A Beating Electromechanically Active Scaffold for Cardiac Tissue Engineering

2016 ◽  
Vol 5 (12) ◽  
pp. 1471-1480 ◽  
Author(s):  
Amy Gelmi ◽  
Artur Cieslar-Pobuda ◽  
Ebo de Muinck ◽  
Marek Los ◽  
Mehrdad Rafat ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mechanical Stimulation ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Stimulation Of
Sign in / Sign up

Export Citation Format

Share Document