Protocol for Isolation, Screening, and Cultivation of Asphaltene-Degrading Microorganism

Author(s):  
Seyed Morteza Zamir ◽  
Tina Tavassoli ◽  
Seyed Abbas Shojaosadati
2021 ◽  
Author(s):  
Chen Zhang ◽  
Gangqin Shu ◽  
Hui Wang ◽  
Daojie Li ◽  
Yuan Xü ◽  
...  

Abstract The effectiveness of the classic screening strategy was verified, by duplicating and verifying the degradation of gossypol by the Aspergillus niger. It can reduce the free gossypol content through biosorption but has no effect on the total gossypol content and cannot effectively degrade gossypol. And the most interesting thing we found the strain can secrete agarase, utilise agar as carbon source. In this case, that will mislead researchers and lead them to make wrong judgments. That turns out the usual methods of previous screening strategies are not rigorous enough, the classic screening method has defect in screening toxin-degrading strain, so agar control group should be added. In this study, some suggestions are put forward to optimise the same type of experiments and broaden the idea of detoxification by microorganisms and provide reference for screening effective toxin-degrading microorganisms.


2014 ◽  
Vol 69 ◽  
pp. 249-254 ◽  
Author(s):  
C. Jayashree ◽  
P. Arulazhagan ◽  
S. Adish Kumar ◽  
S. Kaliappan ◽  
Ick Tae Yeom ◽  
...  

1999 ◽  
Vol 1999 (1) ◽  
pp. 1039-1042 ◽  
Author(s):  
Francesco Crescenzi ◽  
Marcello Camilli ◽  
Eugenio Fascetti ◽  
Filippo Porcelli ◽  
Giulio Prosperi ◽  
...  

ABSTRACT Biological degradation of a light crude dispersed in sea water by a surfactant produced by an hydrocarbon degrading microorganism has been monitored in laboratory tests. Oligotrophic natural sea water was used with no additions. Results showed that the oil dispersed by the biosurfactant was more easily degraded than chemically dispersed oil. In adhesion tests it has been found that the number of microbial cells adhering to a water/hexadecane interface increases in presence of the biosurfactant. It is suggested that the biodegradation enhancement may be linked to a promoting action carried by the biosurfactant on the adhesion of degrading microorganisms onto the surface of the oil.


1988 ◽  
pp. 443-444
Author(s):  
L. W. Barnthouse ◽  
A. V. Palumbo ◽  
V. S. Tripath ◽  
C. D. Little

1997 ◽  
Vol 43 (6) ◽  
pp. 595-598 ◽  
Author(s):  
B. Uma ◽  
S. Sandhya

A gram-positive, pyridine-degrading microorganism identified as Bacillus coagulans has been isolated from contaminated soil by enrichment culture technique. Pyridine was used as sole source of carbon, nitrogen, and energy. Bacillus coagulans has a unique potential to reduce nitrogen from aromatic ring to ammonia and subsequently heterotrophically to nitrite and nitrate. The maximum degradation of pyridine was 94.1% within 72 h at 30 °C with a 7.57-h doubling time. The study suggests possible existence of aromatic degradation and heterotrophic nitrification in Bacillus coagulans.Key words: pyridine degradation, heterotrophic nitrification, Bacillus coagulans.


2004 ◽  
Vol 97 (2) ◽  
pp. 131-133 ◽  
Author(s):  
Nobuki Hayase ◽  
Hidetoshi Yano ◽  
Emi Kudoh ◽  
Chikara Tsutsumi ◽  
Kazutoshi Ushio ◽  
...  

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