scholarly journals Single-particle electron microscopy in the study of membrane protein structure

Microscopy ◽  
2015 ◽  
Vol 65 (1) ◽  
pp. 81-96 ◽  
Author(s):  
Rita De Zorzi ◽  
Wei Mi ◽  
Maofu Liao ◽  
Thomas Walz
Keyword(s):  
Electron Microscopy ◽  
Protein Structure ◽  
Membrane Protein ◽  
Single Particle ◽  
Membrane Protein Structure ◽  
Single Particle Electron Microscopy

scholarly journals Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure

2018 ◽  
Vol 1860 (2) ◽  
pp. 378-383 ◽  
Author(s):  
Mayuriben Parmar ◽  
Shaun Rawson ◽  
Charlotte A. Scarff ◽  
Adrian Goldman ◽  
Timothy R. Dafforn ◽  
...  
Keyword(s):  
Protein Structure ◽  
Membrane Protein ◽  
Single Particle ◽  
Membrane Protein Structure

scholarly journals Structure and assembly of CAV1 8S complexes revealed by single particle electron microscopy

2020 ◽  
Vol 6 (49) ◽  
pp. eabc6185
Author(s):  
Bing Han ◽  
Jason C. Porta ◽  
Jessica L. Hanks ◽  
Yelena Peskova ◽  
Elad Binshtein ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Membrane Protein ◽  
Single Particle ◽  
Structural Organization ◽  
Building Blocks ◽  
Structural Features ◽  
Outer Ring ◽  
Current Evidence ◽  
Caveolin 1 ◽  
Single Particle Electron Microscopy

Highly stable oligomeric complexes of the monotopic membrane protein caveolin serve as fundamental building blocks of caveolae. Current evidence suggests these complexes are disc shaped, but the details of their structural organization and how they assemble are poorly understood. Here, we address these questions using single particle electron microscopy of negatively stained recombinant 8S complexes of human caveolin 1. We show that 8S complexes are toroidal structures ~15 nm in diameter that consist of an outer ring, an inner ring, and central protruding stalk. Moreover, we map the position of the N and C termini and determine their role in complex assembly, and visualize the 8S complexes in heterologous caveolae. Our findings provide critical insights into the structural features of 8S complexes and allow us to propose a model for how these highly stable membrane-embedded complexes are generated.

scholarly journals Structure and assembly of CAV1 8S complexes revealed by single particle electron microscopy

2020 ◽  
Author(s):  
Bing Han ◽  
Jason C. Porta ◽  
Jessica L. Hanks ◽  
Yelena Peskova ◽  
Elad Binshtein ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Membrane Protein ◽  
Single Particle ◽  
Structural Organization ◽  
Building Blocks ◽  
Structural Features ◽  
Current Evidence ◽  
New Model ◽  
Caveolin 1 ◽  
Single Particle Electron Microscopy

AbstractHighly stable oligomeric complexes of the monotopic membrane protein caveolin serve as fundamental building blocks of caveolae. Current evidence suggests these complexes are disc shaped, but the details of their structural organization and how they assemble are poorly understood. Here, we address these questions using single particle electron microscopy of negatively stained recombinant 8S complexes of human Caveolin-1. We show that 8S complexes are toroidal structures ~15 nm in diameter that consist of an outer ring, an inner ring, and central protruding stalk. Moreover, we map the position of the N- and C-termini and determine their role in complex assembly, and visualize the 8S complexes in heterologous caveolae. Our findings provide critical insights into the structural features of 8S complexes and allow us to propose a new model for how these highly stable membrane-embedded complexes are generated.

Order-disorder transitions of cytoplasmic N-termini in the mechanisms of P-type ATPases

2020 ◽  
Author(s):  
Khondker Rufaka Hossain ◽  
Daniel Clayton ◽  
Sophia C Goodchild ◽  
Alison Rodger ◽  
Richard James Payne ◽  
...  
Keyword(s):  
Protein Structure ◽  
Membrane Protein ◽  
Structure And Function ◽  
Membrane Protein Structure ◽  
Protein Structure And Function ◽  
Lipid Environment ◽  
Membrane Pumps ◽  
And Function ◽  
P Type

Membrane protein structure and function are modulated via interactions with their lipid environment. This is particularly true for the integral membrane pumps, the P-type ATPases. These ATPases play vital roles...

Modular Imaging Scaffold for Single-Particle Electron Microscopy

ACS Nano ◽  
2021 ◽  
Author(s):  
Nesrine Aissaoui ◽  
Josephine Lai-Kee-Him ◽  
Allan Mills ◽  
Nathalie Declerck ◽  
Zakia Morichaud ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy
Sign in / Sign up

Export Citation Format

Share Document