Label-Free Quantitative Phosphoproteomics for Algae

2020 ◽  
pp. 197-211
Author(s):  
Megan M. Ford ◽  
Sheldon R. Lawrence ◽  
Emily G. Werth ◽  
Evan W. McConnell ◽  
Leslie M. Hicks
Keyword(s):  
Label Free ◽  
Quantitative Phosphoproteomics

scholarly journals Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Otto Kauko ◽  
Teemu Daniel Laajala ◽  
Mikael Jumppanen ◽  
Petteri Hintsanen ◽  
Veronika Suni ◽  
...  
Keyword(s):  
Label Free ◽  
Quantitative Phosphoproteomics

Label-Free Quantitative Phosphoproteomics Reveals Signaling Dynamics Involved in Embryogenic Competence Acquisition in Sugarcane

2020 ◽  
Vol 19 (10) ◽  
pp. 4145-4157
Author(s):  
Felipe A. Almeida ◽  
Lucas Z. Passamani ◽  
Claudete Santa-Catarina ◽  
Brian P. Mooney ◽  
Jay J. Thelen ◽  
...  
Keyword(s):  
Label Free ◽  
Embryogenic Competence ◽  
Signaling Dynamics ◽  
Competence Acquisition ◽  
Quantitative Phosphoproteomics

scholarly journals Identification of Novel Physiological Substrates ofMycobacterium bovisBCG Protein Kinase G (PknG) by Label-free Quantitative Phosphoproteomics

2018 ◽  
Vol 17 (7) ◽  
pp. 1365-1377 ◽  
Author(s):  
Kehilwe C. Nakedi ◽  
Bridget Calder ◽  
Mousumi Banerjee ◽  
Alexander Giddey ◽  
Andrew J. M. Nel ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase G ◽  
Label Free ◽  
Quantitative Phosphoproteomics ◽  
Physiological Substrates

scholarly journals Automated, Reproducible, Titania-Based Phosphopeptide Enrichment Strategy for Label-Free Quantitative Phosphoproteomics

2013 ◽  
pp. jbt.13-2401-002 ◽  
Author(s):  
Brenna McJury Richardson ◽  
Erik J. Soderblom ◽  
J. Will Thompson ◽  
M. Arthur Moseley
Keyword(s):  
Phosphopeptide Enrichment ◽  
Label Free ◽  
Enrichment Strategy ◽  
Quantitative Phosphoproteomics

scholarly journals Characterization of a TiO2 enrichment method for label-free quantitative phosphoproteomics

Methods ◽  
2011 ◽  
Vol 54 (4) ◽  
pp. 370-378 ◽  
Author(s):  
Alex Montoya ◽  
Luisa Beltran ◽  
Pedro Casado ◽  
Juan-Carlos Rodríguez-Prados ◽  
Pedro R. Cutillas
Keyword(s):  
Label Free ◽  
Enrichment Method ◽  
Quantitative Phosphoproteomics

scholarly journals Discovery of Mouse Spleen Signaling Responses to Anthrax using Label-Free Quantitative Phosphoproteomics via Mass Spectrometry

2010 ◽  
Vol 10 (3) ◽  
pp. M110.000927 ◽  
Author(s):  
Nathan P. Manes ◽  
Li Dong ◽  
Weidong Zhou ◽  
Xiuxia Du ◽  
Nikitha Reghu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Spleen ◽  
Label Free ◽  
Quantitative Phosphoproteomics

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

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