A Comparative Study of RNA Polymerase II Transcription Machinery in Yeasts

2009 ◽  
pp. 405-432
Author(s):  
Nimisha Sharma ◽  
Surbhi Mehta
Keyword(s):  
Comparative Study ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Machinery ◽  
Rna Polymerase Ii Transcription

scholarly journals Therapeutic Targeting of the General RNA Polymerase II Transcription Machinery

2020 ◽  
Vol 21 (9) ◽  
pp. 3354 ◽  
Author(s):  
Ryan D. Martin ◽  
Terence E. Hébert ◽  
Jason C. Tanny
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Complex Diseases ◽  
Cellular Models ◽  
Transcription Machinery ◽  
Transcription Inhibitors ◽  
Preclinical And Clinical Studies ◽  
Rna Polymerase Ii Transcription ◽  
Rnapii Transcription

Inhibitors targeting the general RNA polymerase II (RNAPII) transcription machinery are candidate therapeutics in cancer and other complex diseases. Here, we review the molecular targets and mechanisms of action of these compounds, framing them within the steps of RNAPII transcription. We discuss the effects of transcription inhibitors in vitro and in cellular models (with an emphasis on cancer), as well as their efficacy in preclinical and clinical studies. We also discuss the rationale for inhibiting broadly acting transcriptional regulators or RNAPII itself in complex diseases.

scholarly journals Quantitation of the RNA Polymerase II Transcription Machinery in Yeast

2001 ◽  
Vol 276 (50) ◽  
pp. 47150-47153 ◽  
Author(s):  
Tilman Borggrefe ◽  
Ralph Davis ◽  
Avital Bareket-Samish ◽  
Roger D. Kornberg
Keyword(s):  
Quantitative Analysis ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Blot Analysis ◽  
Dot Blot ◽  
Yeast Proteome ◽  
Transcription Machinery ◽  
Large Complex ◽  
Dot Blot Analysis ◽  
Rna Polymerase Ii Transcription

TAP tags and dot blot analysis have been used to measure the amounts of RNA polymerase II transcription proteins in crude yeast extracts. The measurements showed comparable amounts of RNA polymerase II, TFIIE, and TFIIF, lower levels of TBP and TFIIB, and still lower levels of Mediator and TFIIH. These findings are consistent with the presumed roles of the transcription proteins, but do not support the idea of their recruitment in a single large complex to RNA polymerase II promoters. The approach employed here can be readily extended to quantitative analysis of the entire yeast proteome.

The Eukaryotic Gene Transcription Machinery

2001 ◽  
Vol 382 (8) ◽  
pp. 1103-1107 ◽  
Author(s):  
Roger D. Kornberg
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Gene Transcription ◽  
Atomic Resolution ◽  
Entire System ◽  
Transcription Machinery ◽  
General Transcription Factors ◽  
Eukaryotic Gene ◽  
Rna Polymerase Ii Transcription

Abstract Seven purified proteins may be combined to reconstitute regulated, promoterdependent RNA polymerase II transcription: five general transcription factors, Mediator, and RNA polymerase II. The entire system has been conserved across species from yeast to humans. The structure of RNA polymerase II, consisting of 10 polypeptides with a mass of about 500 kDa, has been determined at atomic resolution. On the basis of this structure, that of an actively transcribing RNA polymerase II complex has been determined as well.

scholarly journals Structure and mechanism of the RNA polymerase II transcription machinery

2020 ◽  
Vol 34 (7-8) ◽  
pp. 465-488 ◽  
Author(s):  
Allison C. Schier ◽  
Dylan J. Taatjes
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Machinery ◽  
Rna Polymerase Ii Transcription

scholarly journals A Single-Molecule Surface-Based Platform to Detect the Assembly and Function of the Human RNA Polymerase II Transcription Machinery

Structure ◽  
2020 ◽  
Vol 28 (12) ◽  
pp. 1337-1343.e4 ◽  
Author(s):  
Sang Ryul Park ◽  
Jesse Hauver ◽  
Yunxiang Zhang ◽  
Andrey Revyakin ◽  
Robert A. Coleman ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Single Molecule ◽  
Transcription Machinery ◽  
And Function ◽  
Rna Polymerase Ii Transcription ◽  
Molecule Surface
Sign in / Sign up

Export Citation Format

Share Document