Industrial Enzyme Use

β-Galactosidase is an important industrial enzyme in the hydrolysis of milk and whey lactose. The enzymatic hydrolysis of lactose allows to avoid health and environmental problems posed by this disaccharide. In addition, this enzyme catalyses the formation of galacto-oligosaccharides, which are prebiotic additives for the so-called “healthy foods”. β-Galactosidase is one of the relatively few enzymes that have been used in large-scale processes in both free and immobilised forms. This article presents a review of recent trends in immobilisation of β-galactosidase and their application in food industry.

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Genome Assembly of Chryseobacterium polytrichastri ERMR1:04, a Psychrotolerant Bacterium with Cold Active Proteases, Isolated from East Rathong Glacier in India

Genome Announcements ◽

10.1128/genomea.01305-15 ◽

2015 ◽

Vol 3 (6) ◽

Cited By ~ 8

Author(s):

Rakshak Kumar ◽

Dharam Singh ◽

Mohit Kumar Swarnkar ◽

Anil Kumar Singh ◽

Sanjay Kumar

Keyword(s):

Genome Assembly ◽

Cold Environment ◽

Sikkim Himalaya ◽

Industrial Enzyme ◽

Cold Active ◽

Psychrotolerant Bacterium ◽

Insight Into

We report here the genome assembly of a psychrotolerant bacterium, Chryseobacterium polytrichastri ERMR1:04, which secretes cold-active proteases. The bacterium was isolated from a pristine location, the East Rathong Glacier in the Sikkim Himalaya. The 5.53-Mb genome provides insight into the cold-active industrial enzyme and adaptation in the cold environment.

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Determination of Neutral Lactase Activity in Industrial Enzyme Preparations by a Colorimetric Enzymatic Method: Collaborative Study

Journal of AOAC International ◽

10.1093/jaoac/82.1.112 ◽

1999 ◽

Vol 82 (1) ◽

pp. 112-118 ◽

Cited By ~ 3

Author(s):

Adrianus J Engelen ◽

Peter H G Randsdorp ◽

R Cassaigne ◽

S M Dutta ◽

M Harboe ◽

...

Keyword(s):

Standard Deviation ◽

Relative Standard Deviation ◽

Colorimetric Assay ◽

Collaborative Study ◽

Enzymatic Method ◽

Lactase Activity ◽

Method Performance ◽

Enzyme Preparations ◽

Industrial Enzyme ◽

Relative Standard

Abstract Thirteen laboratories participated in a collaborative study to validate a colorimetric assay for determining neutral lactase activity in industrial enzyme preparations. Each laboratory received 5 duplicate samples with activity levels of 2000 and 5000 neutral lactase units provided by 4 commercial suppliers. Two laboratories did not return results. Method performance was calculated according to AOAC guidelines. From the 11 remaining laboratories, 3 were excluded from statistical analysis because of invalid data determined during initial review by Youden pair, value versus laboratory. Repeatability relative standard deviation (RSDr) values ranged from 3.20 to 8.62%, and reproducibility relative standard deviation (RSDR) values ranged from 8.77 to 16.35%. With outliers excluded, RSDr values ranged from 2.94 to 5.01%, and RSDRvalues ranged from 7.50 to 13.84%. The colorimetric enzymatic method for determining neutral lactase activity in industrial enzyme preparations has been adopted first action by AOAC INTERNATIONAL.

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