Utilization of Lignocellulosic Agro-Waste as an Alternative Carbon Source for Industrial Enzyme Production

A mixed microbial culture able to transform alachlor at a concentration of 50 μg ml-1was obtained from alachlor-treated soil after an enrichment period of 84 days. The microbial community was composed of seven strains of bacteria. No single isolate was able to utilize alachlor as a sole source of carbon. There was no alachlor left in the enriched culture after a 14-day incubation, but only 12% of the14C-ring-labeled alachlor was converted to14CO2through ring cleavage during 14 days in the basal medium amended with alachlor as a sole carbon source. The presence of sucrose as an alternative carbon source decreased the mineralization potential of the enriched culture, but sucrose increased the mineralizing ability of a three-member mixed culture. Thin-layer chromatographic analysis showed that there were four unidentified metabolites of alachlor produced by the enriched culture. Sucrose decreased the amount of two of the four metabolites. The absence of a noticeable decline in radioactivity beyond the initial 12% suggested that the side chain of alachlor was utilized as carbon source by the enriched culture. Little difference in radioactivity between growth medium and cell-free supernatant of the growth medium suggested that the carbon in the ring was not incorporated into the cells of the degrading microorganisms.

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Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.8634 ◽

2015 ◽

Vol 19 (1) ◽

pp. 56

Author(s):

Sebastian Margino ◽

Erni Martani ◽

Andriessa Prameswara

Keyword(s):

Carbon Source ◽

Internal Energy ◽

Organic Polymer ◽

Ftir Analysis ◽

Phb Production ◽

Alternative Carbon Source ◽

Arrowroot Starch ◽

Physiological Characters

Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB ispotential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. Thedevelopment of PHB production using starch as an alternative carbon source has been conducted to reducethe dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processingsite were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of thestudy showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB rangedfrom 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 andafter optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubationtime. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed asMicrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicatedas poly-β- hydroxybutyrate (PHB)

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Wild type Bacillus subtilis treated with ethyl methyl sulphonate produced catabolite insensitive mutants with improved cellulase production

10.21203/rs.3.rs-278081/v1 ◽

2021 ◽

Author(s):

Oladipo Olaniyi

Keyword(s):

Bacillus Subtilis ◽

Carbon Source ◽

Enzyme Production ◽

Cellulase Activity ◽

Cellulase Production ◽

Minimal Salt Medium ◽

Salt Medium ◽

Production Medium ◽

Wild Type ◽

Ethyl Methyl

Abstract The goal of this present investigation was to mutagenize Bacillus subtilis with Ethyl Methyl Sulphonate (EMS), screen the mutants for cellulase production and evaluate the influence of different glucose concentrations on their cellulase production potentials. The wild type B. subtilis was treated with 20, 40, 60 and 80 µl of EMS and the mutants generated were screened for cellulase production in minimal salt medium containing carboxylmethylcellulose (CMC) as the carbon source. Quantitatively, cellulase activity and protein contents were determined by dinitrosalicylic acid and Lowry methods respectively. Seven mutants were developed from each of the EMS concentration bringing the total to twenty-eight from all the concentrations. Approximately 14 and 57% of the mutants developed from 40 and 60µl of EMS had higher cellulase activities than the wild type, while none of the mutants developed from 20 and 80 µl of EMS had better activities than the wild type. The supplementation of 0.2, 0.5, 1.0 and 1.5% glucose in enzyme production medium caused approximately 100, 14, 29 and 14% cellulase repression respectively in the mutants developed from 60µl EMS. Mutants MSSS02 and MSSS05 were considered as catabolite insensitive mutants because their cellulase production were enhanced in comparison to wild type.

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Biodiesel production from heterotrophic oleaginous microalga Thraustochytrium sp. BM2 with enhanced lipid accumulation using crude glycerol as alternative carbon source

Bioresource Technology ◽

10.1016/j.biortech.2020.123113 ◽

2020 ◽

Vol 306 ◽

pp. 123113 ◽

Cited By ~ 8

Author(s):

Chun-Yen Chen ◽

Meng-Hsiu Lee ◽

Yoong Kit Leong ◽

Jo-Shu Chang ◽

Duu-Jong Lee

Keyword(s):

Carbon Source ◽

Lipid Accumulation ◽

Crude Glycerol ◽

Biodiesel Production ◽

Alternative Carbon Source

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Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

Hoehnea ◽

10.1590/2236-8906-40/2017 ◽

2018 ◽

Vol 45 (1) ◽

pp. 134-142 ◽

Cited By ~ 5

Author(s):

Flaviane Lopes Ferreira ◽

Cesar Barretta Dall'Antonia ◽

Emerson Andrade Shiga ◽

Larissa Juliani Alvim ◽

Rosemeire Aparecida Bom Pessoni

Keyword(s):

Carbon Source ◽

Enzymatic Activity ◽

Sugarcane Bagasse ◽

Enzyme Production ◽

Sole Carbon Source ◽

Sole Source ◽

Maximum Activity ◽

Liquid Media ◽

Biotechnological Potential ◽

Efficient Producer

ABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon.

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A defined growth medium for the production of chloroperoxidase by Caldariomyces fumago

Canadian Journal of Microbiology ◽

10.1139/m81-199 ◽

1981 ◽

Vol 27 (12) ◽

pp. 1298-1305 ◽

Cited By ~ 35

Author(s):

Michael A. Pickard

Keyword(s):

Enzyme Activity ◽

Carbon Source ◽

Growth Medium ◽

Enzyme Production ◽

Defined Medium ◽

Malt Extract ◽

Caldariomyces Fumago ◽

High Enzyme ◽

Extract Medium ◽

Malt Extract Medium

Ten strains of Caldariomyces fumago and related fungi were found to produce extracellular chloroperoxidase when grown on a glucose – malt extract medium. High enzyme levels and pigment production were observed for C. fumago ATCC 16373 and C. fumago CMI 89362. Removal of malt extract from the medium and the replacement of glucose by fructose as the carbon source provided a defined medium which, by comparison with the complex medium, produced the following results with both fungal strains. Chloroperoxidase was produced to similar levels, with maximum production after 6 days rather than 12 days of growth; pigmentation of the medium was reduced by 90% and the pH of the medium remained constant, thus stabilizing enzyme activity. Addition of urea or proline as a nitrogen supplement to nitrate enhanced enzyme production by strain CMI 89362. Comparison of the two strains indicated that CMI 89362 produced higher levels of chloroperoxidase than ATCC 16373.

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Stimulation of dextranase production by oxidized dextran

Canadian Journal of Microbiology ◽

10.1139/m70-141 ◽

1970 ◽

Vol 16 (9) ◽

pp. 841-844 ◽

Cited By ~ 9

Author(s):

Robert G. Brown

Keyword(s):

Carbon Source ◽

Nitrogen Source ◽

Enzyme Production ◽

Inorganic Nitrogen ◽

Penicillium Funiculosum ◽

Inorganic Nitrogen Source ◽

Oxidized Dextran ◽

Excess Glucose ◽

Stimulation Of

Penicillium funiculosum, Penicillium lilacinum, and Spicaria violacea produced excellent yields of dextranase if ketodextran replaced dextran as a carbon source. Ketodextrans I and II having degrees of substitution of 2 and 20% respectively were used in this study. P. funiculosum grew equally well on dextran and ketodextran I but less well on ketodextran II. Addition of a readily metabolizable carbohydrate such as glucose, sucrose, or galactose stimulated growth on ketodextran II, resulting in better dextranase production. However, excess glucose reversed this increase in enzyme production. Replacement of an inorganic nitrogen source with an organic one further stimulated dextranase production during growth of P. funiculosum on ketodextran II.

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