A δ13C and δ15N Tracer Study of Nutrition in Aquaculture: Penaeus vannamei in a Pond Growout System

Author(s):  
P. L. Parker ◽  
R. K. Anderson ◽  
A. Lawrence
2019 ◽  
Author(s):  
Marko J. Spasojevic ◽  
Sören Weber1

Stable carbon (C) and nitrogen (N) isotopes in plants are important indicators of plant water use efficiency and N acquisition strategies. While often regarded as being under environmental control, there is growing evidence that evolutionary history may also shape variation in stable isotope ratios (δ13C and δ15N) among plant species. Here we examined patterns of foliar δ13C and δ15N in alpine tundra for 59 species in 20 plant families. To assess the importance of environmental controls and evolutionary history, we examined if average δ13C and δ15N predictably differed among habitat types, if individual species exhibited intraspecific trait variation (ITV) in δ13C and δ15N, and if there were a significant phylogenetic signal in δ13C and δ15N. We found that variation among habitat types in both δ13C and δ15N mirrored well-known patterns of water and nitrogen limitation. Conversely, we also found that 40% of species exhibited no ITV in δ13C and 35% of species exhibited no ITV in δ15N, suggesting that some species are under stronger evolutionary control. However, we only found a modest signal of phylogenetic conservatism in δ13C and no phylogenetic signal in δ15N suggesting that shared ancestry is a weaker driver of tundra wide variation in stable isotopes. Together, our results suggest that both evolutionary history and local environmental conditions play a role in determining variation in δ13C and δ15N and that considering both factors can help with interpreting isotope patterns in nature and with predicting which species may be able to respond to rapidly changing environmental conditions.


2018 ◽  
Author(s):  
Pedro Abanador ◽  
Marilou Pagsibigan ◽  
Violeta Reyes

2016 ◽  
Author(s):  
Caitlin T. McManimon ◽  
◽  
David P. Gillikin ◽  
William B. Ouimet ◽  
Michael T. Hren ◽  
...  

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
L. F. Aranguren Caro ◽  
F. Alghamdi ◽  
K. De Belder ◽  
J. Lin ◽  
H. N. Mai ◽  
...  

Abstract Background Enterocytozoon hepatopenaei (EHP) is an enteric pathogen that affects Penaeus vannamei and Penaeus monodon shrimp in many SE Asian countries. In the western hemisphere, EHP was reported for the first time in 2016 in farmed P. vannamei in Venezuela. Anecdotal evidence suggests that EHP is more prevalent in grow-out ponds where the salinity is high (> 15 parts per thousand (ppt)) compared to grow-out ponds with low salinities (< 5 ppt). Considering that P. vannamei is an euryhaline species, we were interested in knowing if EHP can propagate in P. vannamei in low salinities. Results In this study, we described an experimental infection using fecal strings as a source inoculum. Specific Pathogen Free (SPF) P. vannamei were maintained at three different salinities (2 ppt, 15 ppt, and 30 ppt) while continuously challenged using feces from known EHP-infected P. vannamei over a period of 3 weeks. The fecal strings, used as a source of EHP inocula in the challenges, was sufficient to elicit an infection in shrimp maintained at the three salinities. The infectivity of EHP in shrimp reared at 2 ppt, 15 ppt, and 30 ppt salinities was confirmed by PCR and histopathology. The prevalence and the severity of the EHP infection was higher at 30 ppt than at 2 ppt and 15 ppt. Conclusion The data suggests that fecal strings are a reliable source of EHP inoculum to conduct experimental challenges via the fecal-oral route. An EHP infection can occur at a salinity as low as 2 ppt, however, the prevalence and the severity of the EHP infection is higher at a salinity of 30 ppt.


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