Coordinate Regulation of Zinc Metabolism and Metallothionein Gene Expression by cAMP, Interleukin-1 and Dietary Copper and Zinc

1988 ◽  
pp. 281-285 ◽  
Author(s):  
Robert J. Cousins ◽  
Michael A. Dunn ◽  
Teresa L. Blalock ◽  
Annette S. Leinart
Keyword(s):  
Gene Expression ◽  
Interleukin 1 ◽  
Zinc Metabolism ◽  
Dietary Copper ◽  
Coordinate Regulation ◽  
Metallothionein Gene ◽  
Copper And Zinc

Coordinate regulation of zinc metabolism and metallothionein gene expression in rats

1986 ◽  
Vol 251 (6) ◽  
pp. E688-E694 ◽  
Author(s):  
R. J. Cousins ◽  
M. A. Dunn ◽  
A. S. Leinart ◽  
K. C. Yedinak ◽  
R. A. DiSilvestro
Keyword(s):  
Gene Expression ◽  
Acute Stress ◽  
Blot Hybridization ◽  
Serum Zinc ◽  
Zinc Metabolism ◽  
Dibutyryl Camp ◽  
Dot Blot ◽  
Potent Effect ◽  
Coordinate Regulation ◽  
Metallothionein Gene

Regulation of zinc metabolism by dibutyryl cAMP, glucagon, and epinephrine was examined in rats fed adequate amounts of zinc. Dibutyryl cAMP, epinephrine, and glucagon each produced an increase in liver metallothionein levels by 10 h after they were first administered. The increase in liver metallothionein was inversely related to the serum zinc concentration. Treatment with dexamethasone, a glucocorticoid, accentuated these effects to some extent. Both metallothionein I and II were induced by dibutyryl cAMP and glucagon. Levels of metallothionein mRNA in total liver RNA extracts were measured by dot blot hybridization using a synthetic 21-base oligonucleotide complimentary to the 5' region of both the metallothionein I and II genes. Individual administration of dibutyryl cAMP, glucagon, and epinephrine increased the number of metallothionein mRNA molecules per cell by up to fourfold. The data suggest that glucagon and epinephrine are primary regulators of metallothionein gene expression acting at least in part via cAMP. In adrenalectomized rats, glucagon, dibutyryl cAMP, and epinephrine had a less potent effect in terms of metallothionein induction and depression of serum zinc concentrations. These effects were largely restored when dexamethasone was also given. Collectively these data suggest that changes in zinc metabolism associated with acute stress involve coordinate regulation mediated by many factors, including glucocorticoids and cAMP.

scholarly journals Interleukin 1 regulates human metallothionein gene expression.

1985 ◽  
Vol 5 (10) ◽  
pp. 2866-2869 ◽  
Author(s):  
M Karin ◽  
R J Imbra ◽  
A Heguy ◽  
G Wong
Keyword(s):  
Gene Expression ◽  
Free Radical ◽  
Hydroxyl Radicals ◽  
Interleukin 1 ◽  
Free Radical Scavenger ◽  
Human Cells ◽  
Radical Scavenger ◽  
Protective Response ◽  
Metallothionein Gene ◽  
Cultured Human Cells

Incubation of cultured human cells with interleukin 1 leads to increased expression of the human metallothionein-IIA gene. Recently, metallothionein has been shown to be an efficient free radical scavenger, and induction by interleukin 1 may be part of a protective response to minimize damage by hydroxyl radicals.

Interleukin 1 regulates human metallothionein gene expression

1985 ◽  
Vol 5 (10) ◽  
pp. 2866-2869
Author(s):  
M Karin ◽  
R J Imbra ◽  
A Heguy ◽  
G Wong
Keyword(s):  
Gene Expression ◽  
Free Radical ◽  
Hydroxyl Radicals ◽  
Interleukin 1 ◽  
Free Radical Scavenger ◽  
Human Cells ◽  
Radical Scavenger ◽  
Protective Response ◽  
Metallothionein Gene ◽  
Cultured Human Cells

Incubation of cultured human cells with interleukin 1 leads to increased expression of the human metallothionein-IIA gene. Recently, metallothionein has been shown to be an efficient free radical scavenger, and induction by interleukin 1 may be part of a protective response to minimize damage by hydroxyl radicals.

Metallothionein gene expression in the intestinal cell: modulation of mRNA and protein synthesis by copper and zinc

1991 ◽  
Vol 19 (3) ◽  
pp. 283S-283S ◽  
Author(s):  
MARIA LAURA SCARINO ◽  
ROBERTA POVERINI ◽  
GIUSEPPE DI LULLO ◽  
GIOVANNA BISES
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Intestinal Cell ◽  
Metallothionein Gene ◽  
Copper And Zinc

Local mechanisms for acupoint sensitization in gall bladder meridian of foot shaoyang-a gene chip study

2019 ◽  
Vol 44 (2) ◽  
pp. 77-87
Author(s):  
Koichi Ishida ◽  
Liyue Qin ◽  
Ting Wang ◽  
Ying Lei ◽  
Weiwei Hu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gall Bladder ◽  
Interleukin 1 ◽  
Gene Chip ◽  
Molecular Evidence ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Bladder Meridian ◽  
Integrin Linked Kinase ◽  
Necrosis Factor

Acupuncture manipulations are clinically important to traditional Chinese medicine, yet the biological mechanisms have not been fully understood. This study aimed to investigate continuous stimulation-induced gene expression changes at stimulated and non-stimulated adjacent acupoints in the same meridian. Catgut embedding into acupoint (CEP) was conducted at acupoint Yanglingquan (gall bladder meridian of foot-shaoyang 34, GB34) of Sprague Dawley rats once or continuously for eight weeks, and gene expression changes at GB34 were assessed by gene chip array analysis 72 h after the last CEP treatment. A total of 688 genes exhibited opposite changes in expression between the two treatments, and 1,336 genes were regulated only by the eight-week CEP treatment. Ingenuity Pathway Analysis revealed that among these differentially regulated genes by one-time and eight-week CEP treatment, insulin-like growth factor-1 pathway and integrin-linked kinase pathway, and Wnt/~ catenin signaling pathway match the observed gene changes to predicted up/down regulation patterns. Upstream analysis further predicted six molecules, namely, tumor necrosis factor, interleukin 1~, interleukin la, kallikrein-related peptidase 5, protein kinase Ca, and catenin ~1. On the other hand, continuous eight-week CEP stimulation at acupoint Xuanzhong (GB39) caused similar changes in the expression of 32 genes at acupoints GB34 and Fengshi (GB31) on the same meridian. Taken together, our results provide the first molecular evidence for the local acupoints' mechanisms for acupoint sensitization theory, and implicate the existence of signaling pathways, either direct or indirect, between acupoints within the meridian GB.

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