Integrated Control of the Sugar Beet Cyst Nematode

Sugar beet cyst nematode (SBCN, Heterodera schachtii) is an important nematode that causes significant yield losses of 25–50% or more in most areas of sugar beet production worldwide. Rapid and accurate identification of this species is essential to support decisions on pest management. However, the difference between H. schachtii and other Heterodera spp. based on morphology is a challenging task. In the present study, a SCAR-PCR assay was developed to identify and differentiate H. schachtii in infected root and soil samples. H. schachtii-species-specific SCAR-PCR primers OPA06-HsF and OPA06-HsR were designed from the randomly amplified polymorphic DNA (RAPD) marker amplified with random primer OPA06. The developed primers specifically amplify a 922-bp fragment from the target populations but did not amplify DNA from non-target cyst nematodes including Heterodera, Globodera, Cactodera, and other related species tested in this study. The sensitivity detection indicated that 5 × 10−4 of a single cyst, 1/320 of a single second-stage juvenile (J2), or 10 pg of genomic DNA could be detected. The assay accurately identifies the different stages of H. schachtii in sugar beet and oilseed rape roots as well as a single J2 in 10 g of soil. Finally, the SCAR-PCR assay detected H. schachtii in seven samples out of the fifteen field samples. The assay will not only be useful for differentiating H. schachtii from mixed populations of Heterodera spp. but also for effective detection of the species directly from infested samples. The assay also requires no expertise in the taxonomy and morphology of the species but serves to improve the diagnosis of H. schachtii in infested fields.

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Impact of Sugar Beet Cyst Nematode, Heterodera schachtii, on Some Physiological Aspects of Two Sugar Beet Cultivars, Nemakill and 7233, in the Rhizosphere Condition

Plant Pathology Journal ◽

10.3923/ppj.2007.60.65 ◽

2007 ◽

Vol 6 (1) ◽

pp. 60-65

Author(s):

A.A. Hojat jalali ◽

H.R. Ghasempour ◽

F. Madadzadeh

Keyword(s):

Sugar Beet ◽

Cyst Nematode ◽

Heterodera Schachtii ◽

Beet Cyst Nematode ◽

Sugar Beet Cyst Nematode

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Sugar beet nematode Heterodera schachtii distribution and harmfulness in Ukraine

Agricultural science and practice ◽

10.15407/agrisp3.03.003 ◽

2016 ◽

Vol 3 (3) ◽

pp. 3-11 ◽

Cited By ~ 1

Author(s):

L. Pylypenko ◽

K. Kalatur ◽

J. Hallmann

Keyword(s):

Sugar Beet ◽

Wide Distribution ◽

Cyst Nematode ◽

Control Measures ◽

Heterodera Schachtii ◽

Prevention Measures ◽

Economic Threshold ◽

Beet Cyst Nematode ◽

Effi Ciency ◽

Sugar Beet Cyst Nematode

Aim. To determine the distribution of sugar beet cyst nematode Heterodera schachtii in Ukraine, to defi ne its population density in soil, the degree of damage and the effi ciency of control measures. Methods. Field survey for soil samples, cyst extraction by fl otation and sieving method, light microscopy. Results. The examination of 12,130 ha of agricultural fi elds in six regions of Ukraine, conducted in 2010–2015, recorded the sugar beet cyst nematode to occur in fi ve of them (Kyiv, Chernihiv, Cherkasy, Khmelnytsky, and Vinnytsia), at a total area of 2,572 ha. Taking into consideration the results of previous nematological surveys, the presence of sugar beet cyst nematode has now been documented for 18 regions of Ukraine. In most regions H. schachtii was present in 10–11 % of the examined sugar and seed producing farms with nematode population densities reaching up to 142,000 eggs and juveniles in 100 cc of soil (710-fold above the economic threshold). Conclusions. The main reasons for this wide distribution of H. schachtii in Ukraine are seen in the negligence of prevention measures, unavailability of documented data on its occurrence (missing surveys), crop rotations with over 20 % of host plants, and unavailability of effi cient nematicides and domestic nematode-resistant sugar beet cultivars.

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