Effects of Workshop Monoclonal Antibodies on the Cytolytic Activity of Alloreactive CD4-Positive T Cell Clones

1986 ◽  
pp. 111-121
Author(s):  
Neal Flomenberg ◽  
Bo Dupont ◽  
Robert W. Knowles
Keyword(s):  
Monoclonal Antibodies ◽  
T Cell ◽  
Cytolytic Activity ◽  
T Cell Clones ◽  
Cell Clones

scholarly journals Analysis at the clonal level of T-cell phenotype and functions in severe aplastic anemia patients

Blood ◽  
1991 ◽  
Vol 78 (5) ◽  
pp. 1268-1274 ◽  
Author(s):  
M Viale ◽  
A Merli ◽  
A Bacigalupo
Keyword(s):  
T Cell ◽  
Aplastic Anemia ◽  
Cytolytic Activity ◽  
Severe Aplastic Anemia ◽  
Cell Phenotype ◽  
T Cell Clones ◽  
Mitogen Stimulation ◽  
Clonal Level ◽  
Cell Clones ◽  
Number Of Patients

Abstract The aim of this study was to analyze at the clonal level the phenotype and functions of T cells from patients with severe aplastic anemia (SAA). For this purpose we studied 175 T-cell clones obtained from peripheral blood (PB) and bone marrow (BM) of four SAA patients and 97 clones from two healthy controls. The percentage of CD8+ T-cell clones obtained from the patients' PB and BM was higher, but not significantly (P = .07 and P = .14, respectively), than that obtained in controls. A higher proportion of T-cell clones from SAA patients exhibited lectin- dependent cytolytic activity and especially natural killer-like activity when compared with controls (PB: P less than .01, P less than .05; BM: P less than .05, P less than .01, respectively). Lymphokine release was tested before and after mitogen stimulation. A number of patients' clones were able to release interferons (IFNs) spontaneously (PB: 28.6% v 0%, P less than .05; BM: 28.6% v 0%, P less than .10). After mitogen stimulation, patients' BM T-cell clones produced IFNs in greater proportions (90.9% v 46.7%, P less than .01) and in greater quantities (PB: 25.5 arbitrary units [AU]/mL v 5.7 AU/mL, P less than .03; BM: 26 AU/mL v 9.1 AU/mL, P = .011) as compared with controls. Tumor necrosis factor (TNF) activity was not found in supernatants of unprimed T-cell clones. After mitogen stimulation, PB T-cell microcultures produced TNF alpha in greater proportions (97.9% v 72.2%, P less than .01) and, also in this case, in greater quantities (PB: 7.2 AU/mL v 1.5 AU/mL, P = .007; BM: 9.9 AU/mL v 1.5 AU/mL, P = .003) than controls. In conclusion, T-cell clones from SAA patients exhibit predominantly a CD8+ phenotype, a greater cytotoxic activity, and can be shown to produce greater quantities of suppressor lymphokines when compared with controls.

scholarly journals Alloreactive cloned T cell lines. I. Interactions between cloned amplifier and cytolytic T cell lines.

1980 ◽  
Vol 151 (4) ◽  
pp. 876-895 ◽  
Author(s):  
A L Glasebrook ◽  
F W Fitch
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Cytolytic Activity ◽  
Surface Proteins ◽  
Target Cells ◽  
Spleen Cells ◽  
T Cell Clones ◽  
Cell Clones ◽  
T Cell Lines ◽  
Cytolytic Cell

Several T cell clones have been derived by limiting dilution of secondary mixed leukocyte culture cells stimulated by H-2- and M locus (Mls)-disparate spleen cells. When examined for the expression of cytolytic activity and the ability to proliferate, these cell clones can be classified into two major categories. One type of cell is noncytolytic; when cultured with irradiated spleen cells, such clones proliferate in response to Mls determinants. Some, but not all, of these clones express Lyt-1 alloantigens. The other type of cell is cytolytic; these clones do not proliferate when cultured with irradiated allogeneic spleen cells unless supernatant fluid (SF) is added. These cytolytic clones express Lyt-2 alloantigens. Some cytolytic clones are specific for H-2Kd and others for H-2Dd alloantigens. Still other cytolytic cell clones exhibit cross-reactive lysis of different H-2-bearing tumor and Con A blast target cells. Noncytolytic T cell clones, when stimulated by Mls antigens, were examined for their ability to promote proliferation of cytolytic T cell clones. All of the noncytolytic cell clones tested were able to promote proliferation of cytolytic cell clones with the concomitant expression of cytolytic activity directed toward the original stimulating alloantigen (H-2d). Amplification of cytolytic activity was dependent upon stimulation of the noncytolytic amplifier T cell clones by Mls antigens. Specific alloantigen (signal 1), however, was not required for proliferation of the cytolytic cell clones; the amplifying signal (signal 2), delivered by the amplifier cell clones, was sufficient alone to promote proliferation of the cytolytic cell clones. Whereas proliferation of the amplifier cells was radiosensitive, the generation of the soluble amplifying signal was radioresistant. Amplification of cytolytic activity was observed when either amplifier cells were physically separated from responding cytolytic cells in Marbrook cultures or when cytolytic cells were cultured with SF collected from amplifier cell cultures. The amplifying factors were neither antigen specific nor strain specific and could be produced by Lyt-1- cells. The availability of cloned T cell lines that retain specific biologic function offers unique opportunities to characterize cell surface proteins and cell-cell interactions.

Human T cell clones with γδ and αβ receptors are differently stimulated by monoclonal antibodies to CD2

1990 ◽  
Vol 129 (2) ◽  
pp. 385-393 ◽  
Author(s):  
Graham Pawelec ◽  
Kurt Schaudt ◽  
Arnika Rehbein ◽  
Daniel Olive ◽  
Hans-Jörg Bühring
Keyword(s):  
Monoclonal Antibodies ◽  
T Cell ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones
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