Cytoskeletal Participation in Stimulated Secretion and Compensatory Apical Plasma Membrane Retrieval in Lacrimal Gland Acinar Cells

2002 ◽  
pp. 199-205 ◽  
Author(s):  
Silvia R. da Costa ◽  
Sofia Andersson ◽  
Francie A. Yarber ◽  
Curtis Okamoto ◽  
Sarah Hamm-Alvarez
Keyword(s):  
Plasma Membrane ◽  
Lacrimal Gland ◽  
Acinar Cells ◽  
Apical Plasma Membrane

scholarly journals Redistribution of a rab3-like GTP-binding protein from secretory granules to the Golgi complex in pancreatic acinar cells during regulated exocytosis

1994 ◽  
Vol 124 (1) ◽  
pp. 43-53 ◽  
Author(s):  
BP Jena ◽  
FD Gumkowski ◽  
EM Konieczko ◽  
GF von Mollard ◽  
R Jahn ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Golgi Complex ◽  
Binding Protein ◽  
Secretory Granules ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Apical Plasma Membrane ◽  
Gtp Binding Protein ◽  
Regulated Exocytosis ◽  
Gtp Binding

Regulated secretion from pancreatic acinar cells occurs by exocytosis of zymogen granules (ZG) at the apical plasmalemma. ZGs originate from the TGN and undergo prolonged maturation and condensation. After exocytosis, the zymogen granule membrane (ZGM) is retrieved from the plasma membrane and ultimately reaches the TGN. In this study, we analyzed the fate of a low M(r) GTP-binding protein during induced exocytosis and membrane retrieval using immunoblots as well as light and electron microscopic immunocytochemistry. This 27-kD protein, identified by a monoclonal antibody that recognizes rab3A and B, may be a novel rab3 isoform. In resting acinar cells, the rab3-like protein was detected primarily on the cytoplasmic face of ZGs, with little labeling of the Golgi complex and no significant labeling of the apical plasmalemma or any other intracellular membranes. Stimulation of pancreatic lobules in vitro by carbamylcholine for 15 min, resulted in massive exocytosis that led to a near doubling of the area of the apical plasma membrane. However, no relocation of the rab3-like protein to the apical plasmalemma was seen. After 3 h of induced exocytosis, during which time approximately 90% of the ZGs is released, the rab3-like protein appeared to translocate to small vesicles and newly forming secretory granules in the TGN. No significant increase of the rab3-like protein was found in the cytosolic fraction at any time during stimulation. Since the protein is not detected on the apical plasmalemma after stimulation, we conclude that recycling may involve a membrane dissociation-association cycle that accompanies regulated exocytosis.

scholarly journals Differential Regulation of the Apical Plasma Membrane Ca2+-ATPase by Protein Kinase A in Parotid Acinar Cells

2007 ◽  
Vol 282 (52) ◽  
pp. 37678-37693 ◽  
Author(s):  
Erin Baggaley ◽  
Stuart McLarnon ◽  
Irma Demeter ◽  
Gabor Varga ◽  
Jason I. E. Bruce
Keyword(s):  
Plasma Membrane ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Acinar Cells ◽  
Differential Regulation ◽  
Apical Plasma Membrane ◽  
Parotid Acinar Cells ◽  
Kinase A

Endocytosis at the apical plasma membrane of pancreatic acinar cells is regulated by tyrosine kinases

1999 ◽  
Vol 276 (2) ◽  
pp. C306-C311 ◽  
Author(s):  
Steven D. Freedman ◽  
Mark H. Katz ◽  
Eliza M. Parker ◽  
Andres Gelrud
Keyword(s):  
Plasma Membrane ◽  
Tyrosine Kinases ◽  
Src Kinase ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Apical Plasma Membrane ◽  
Specific Substrate ◽  
Pancreatic Acini ◽  
Kinase Activation ◽  
Acinar Lumen

We have shown that endocytosis at the apical plasma membrane of pancreatic acinar cells is regulated by the pH of the acinar lumen and is associated with cleavage of GP2, a glycosyl phosphatidylinositol-anchored protein. The aim of this study was to determine the transduction pathway by which endocytosis is activated. Apical endocytosis was studied in rat pancreatic acini by prestimulation with cholecystokinin followed by measurement of horseradish peroxidase (HRP) uptake. Lanthanum, staurosporine, and forskolin had no effect on HRP uptake. Cytochalasin D significantly inhibited endocytosis, indicating a dependence on actin filament integrity. Genistein and the specific tyrphostin inhibitor B42 also inhibited HRP uptake, implicating tyrosine kinases in the regulation of HRP uptake. With the use of an Src kinase-specific substrate, Src kinase activity was temporally related to activation of endocytosis. The tyrosine-dependent phosphorylation of an 85-kDa substrate in both rat and mouse pancreatic acini correlated with Src kinase activation and pH-dependent regulation of HRP uptake. These results indicate that apical endocytosis in acinar cells is associated with tyrosine kinase activation and is dependent on the actin cytoskeleton.

Activation of muscarinic receptors in rat parotid acinar cells induces AQP5 trafficking to nuclei and apical plasma membrane

2015 ◽  
Vol 1850 (4) ◽  
pp. 784-793 ◽  
Author(s):  
Gota Cho ◽  
Aneta M. Bragiel ◽  
Di Wang ◽  
Tomasz D. Pieczonka ◽  
Mariusz T. Skowronski ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Muscarinic Receptors ◽  
Acinar Cells ◽  
Apical Plasma Membrane ◽  
Parotid Acinar Cells ◽  
Rat Parotid
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