Stimulation of ribosomal RNA synthesis during hypertrophic growth of cultured heart cells by phorbol ester

1991 ◽  
pp. 169-177 ◽  
Author(s):  
Takashi Haneda ◽  
Paul J. McDermott
Keyword(s):  
Phorbol Ester ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Heart Cells ◽  
Hypertrophic Growth ◽  
Cultured Heart Cells ◽  
Stimulation Of

scholarly journals Accelerated rates of ribosomal RNA synthesis during growth of contracting heart cells in culture

1989 ◽  
Vol 264 (30) ◽  
pp. 18220-18227
Author(s):  
P J McDermott ◽  
L I Rothblum ◽  
S D Smith ◽  
H E Morgan
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Heart Cells ◽  
Cells In Culture ◽  
Contracting Heart

Stimulation of hypertrophic growth of cultured neonatal rat heart cells through type 1 angiotensin II receptor

1992 ◽  
Vol 24 ◽  
pp. 243
Author(s):  
Setsuya Miyata ◽  
Takashi Haneda ◽  
Jun Fukuzawa ◽  
Kiyotaka Okamoto ◽  
Hiroki Takeda ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Rat Heart ◽  
Neonatal Rat ◽  
Heart Cells ◽  
Angiotensin Ii Receptor ◽  
Hypertrophic Growth ◽  
Neonatal Rat Heart ◽  
Rat Heart Cells ◽  
Stimulation Of

scholarly journals The cyclo-oxygenase inhibitors indomethacin and ibuprofen inhibit the insulin-induced stimulation of ribosomal RNA synthesis in L6 myoblasts

1989 ◽  
Vol 264 (1) ◽  
pp. 101-106 ◽  
Author(s):  
R M Palmer ◽  
G P Campbell ◽  
P F Whitelaw ◽  
D S Brown ◽  
P A Bain ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
High Concentration ◽  
Ribosomal Subunits ◽  
Rrna Species ◽  
Inflammatory Drugs ◽  
Stimulation Of

Insulin stimulated total RNA accretion and the incorporation of [3H]uridine into RNA in L6 skeletal-muscle myoblasts. Incorporation of uridine into the rRNA was measured after either separation of 18 S and 28 S rRNA species by agarose-gel electrophoresis or separation of dissociated 40 S and 60 S ribosomal subunits on sucrose density gradients. Both methods showed a stimulation by insulin of uridine incorporation into the RNA of the two subunits. Two non-steroidal anti-inflammatory drugs, indomethacin and ibuprofen, which inhibit the metabolism of arachidonic acid by the cyclo-oxygenase pathway, inhibited the insulin-induced accretion of total cellular RNA and the incorporation of uridine into the RNA of both ribosomal subunits. The effect of insulin was observed both by using a tracer dose of [3H]uridine (5 microM) and in the presence of a high concentration (1 mM) of uridine to minimize possible changes in intracellular precursor pools. Neither insulin nor indomethacin was found to affect the incorporation of uridine into the total intracellular nucleotide pool, or the conversion of uridine into UTP. The ability of inhibitors of arachidonic acid metabolism to prevent insulin-induced increases in RNA metabolism suggests that a prostaglandin or other eicosanoid is involved in the signal mechanism whereby insulin stimulates RNA synthesis.

scholarly journals STIMULATION BY INSULIN OF RNA SYNTHESIS IN CHICK FIBROBLASTS

1974 ◽  
Vol 60 (1) ◽  
pp. 54-64 ◽  
Author(s):  
Joel B. Baseman ◽  
Domenic Paolini ◽  
Harold Amos
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Polymerase Activity ◽  
Serum Free Medium ◽  
5S Rna ◽  
Fresh Serum ◽  
Protein And Rna Synthesis ◽  
Rna Polymerase Activity ◽  
Kinetics Of ◽  
Stimulation Of

After the addition of insulin to monolayers of chick fibroblasts previously incubated in serum-free medium, the rates of protein and RNA synthesis increase continuously during the first 8–10 h. Little stimulation of DNA synthesis or mitosis results with the addition of insulin alone in contrast to the addition of fresh serum which stimulates both markedly. The stimulation in RNA synthesis does not result from expansion of the nucleotide pool but is correlated with increases in RNA polymerase activity. All major classes of RNA are stimulated; processing of preribosomal RNA to 28S and 18S and the association of this mature RNA with ribosomes appear to occur normally. The kinetics of stimulation of 5S RNA differ from those of the synthesis of 4S and of ribosomal RNA. Insulin and serum appear to affect the synthesis or stability of certain transcripts differentially.

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