Unique Aspects of Nod Gene Expression in Bradyrhizobium Japonicum

1999 ◽  
pp. 115-120
Author(s):  
J. Loh ◽  
J. P.-Y. Yuen ◽  
M. G. Stacey ◽  
G. Stacey
Keyword(s):  
Gene Expression ◽  
Bradyrhizobium Japonicum ◽  
Nod Gene Expression

Regulation of nod gene expression in Bradyrhizobium japonicum

1988 ◽  
Vol 214 (3) ◽  
pp. 420-424 ◽  
Author(s):  
Zsofia Banfalvi ◽  
Anthony Nieuwkoop ◽  
Maria Schell ◽  
Linda Besl ◽  
Gary Stacey
Keyword(s):  
Gene Expression ◽  
Bradyrhizobium Japonicum ◽  
Nod Gene Expression

scholarly journals Photosynthetic Bradyrhizobium Sp. Strain ORS285 Synthesizes 2-O-Methylfucosylated Lipochitooligosaccharides for nod Gene–Dependent Interaction with Aeschynomene Plants

2011 ◽  
Vol 24 (12) ◽  
pp. 1440-1447 ◽  
Author(s):  
Adeline Renier ◽  
Fabienne Maillet ◽  
Joel Fardoux ◽  
Véréna Poinsot ◽  
Eric Giraud ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bradyrhizobium Japonicum ◽  
Culture Supernatant ◽  
Photosynthetic Bacterium ◽  
Lacz Reporter ◽  
Symbiotic Interaction ◽  
Nod Factor ◽  
Bradyrhizobium Sp ◽  
Nod Gene Expression ◽  
Dependent Interaction

Bradyrhizobium sp. strain ORS285 is a photosynthetic bacterium that forms nitrogen-fixing nodules on the roots and stems of tropical aquatic legumes of the Aeschynomene genus. The symbiotic interaction of Bradyrhizobium sp. strain ORS285 with certain Aeschynomene spp. depends on the presence of nodulation (nod) genes whereas the interaction with other species is nod gene independent. To study the nod gene-dependent molecular dialogue between Bradyrhizobium sp. strain ORS285 and Aeschynomene spp., we used a nodB-lacZ reporter strain to monitor the nod gene expression with various flavonoids. The flavanones liquiritigenin and naringenin were found to be the strongest inducers of nod gene expression. Chemical analysis of the culture supernatant of cells grown in the presence of naringenin showed that the major Nod factor produced by Bradyrhizobium sp. strain ORS285 is a modified chitin pentasaccharide molecule with a terminal N-C18:1-glucosamine and with a 2-O-methyl fucose linked to C-6 of the reducing glucosamine. In this respect, the Bradyrhizobium sp. strain ORS285 Nod factor is the same as the major Nod factor produced by the nonphotosynthetic Bradyrhizobium japonicum USDA110 that nodulates the roots of soybean. This suggests a classic nod gene-dependent molecular dialogue between Bradyrhizobium sp. strain ORS285 and certain Aeschynomene spp. This is supported by the fact that B. japonicum USDA110 is able to form N2-fixing nodules on both the roots and stems of Aeschynomene afraspera.

Attachment of IR-active organometallic probe groups to flavonoid inducers of nod gene expression

1999 ◽  
Vol 589 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Andrej V Malkov ◽  
Ljubica Mojovic ◽  
G.Richard Stephenson ◽  
Andrew T Turner ◽  
Colin S Creaser
Keyword(s):  
Gene Expression ◽  
Nod Gene Expression

Expression of Nodulation Genes in Rhizobium and Acid-Sensitivity of Nodule Formation

1989 ◽  
Vol 16 (1) ◽  
pp. 117 ◽  
Author(s):  
AE Richardson ◽  
MA Djordjevic ◽  
BG Rolfe ◽  
RJ Simpson
Keyword(s):  
Gene Expression ◽  
Root Exudates ◽  
Low Ph ◽  
Nodule Formation ◽  
Leguminous Plants ◽  
Acid Sensitivity ◽  
Coordinated Expression ◽  
Rhizobium Spp ◽  
Nod Gene Expression ◽  
Inducing Factors

The formation of nitrogen-fixing nodules on roots of leguminous plants by Rhizobium spp. involves a complex interaction between host plant and symbiont. Successful nodulation requires the coordinated expression of several nodulation (nod) genes in the bacteria. The expression of these genes is induced by flavonoid compounds present in root exudates of host plants. Growth of Rhizobium spp. and formation of nodules on roots of leguminous plants is known to be adversely affected by low pH and factors associated with soil acidity, but the basis of this acid-sensitivity is poorly understood. We consider that poor induction of nodulation gene expression in Rhizobium is a major factor contributing to the acid-sensitivity of nodulation formation. At low pH, induction of nod gene expression in R. leguminosarum biovar trifolii is markedly reduced in the presence of flavone-inducer. Furthermore, inducibility of nod gene expression in R. leguminosarum bv. trifolii is also affected by a net reduction in the concentration of nod gene-inducing factors present in the root exudates of clover seedlings grown in acidic conditions.

A multitude of CRP/FNR-like transcription proteins in Bradyrhizobium japonicum

2006 ◽  
Vol 34 (1) ◽  
pp. 156-159 ◽  
Author(s):  
S. Mesa ◽  
H. Hennecke ◽  
H.-M. Fischer
Keyword(s):  
Gene Expression ◽  
Bradyrhizobium Japonicum ◽  
Sequence Similarity ◽  
Regulatory Protein ◽  
Regulatory System ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Low Oxygen ◽  
Denitrification Gene ◽  
Camp Receptor

In Bradyrhizobium japonicum, the nitrogen-fixing soya bean endosymbiont and facultative denitrifier, three CRP (cAMP receptor protein)/FNR (fumarate and nitrate reductase regulatory protein)-type transcription factors [FixK1, FixK2 and NnrR (nitrite and nitric oxide reductase regulator)] have been studied previously in the context of the regulation of nitrogen fixation and denitrification. The gene expression of both fixK1 and nnrR depends on FixK2, which acts as a key distributor of the ‘low-oxygen’ signal perceived by the two-component regulatory system FixLJ. While the targets for FixK1 are not known, NnrR transduces the nitrogen oxide signal to the level of denitrification gene expression. Besides these three regulators, the complete genome sequence of this organism has revealed the existence of 13 additional CRP/FNR-type proteins whose functions have not yet been studied. Based on sequence similarity and phylogenetic analysis, we discuss in this paper the peculiarities of these additional factors.

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