bradyrhizobium sp
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Pedosphere ◽  
2022 ◽  
Vol 32 (3) ◽  
pp. 438-451
Author(s):  
Kailash Chand KUMAWAT ◽  
Inderjeet SINGH ◽  
Sharon NAGPAL ◽  
Poonam SHARMA ◽  
Rajeev Kumar GUPTA ◽  
...  

Qantu Yachay ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 36-45
Author(s):  
Viane Marytrini Mejia Huaranca ◽  
Gregorio José Arone Gaspar

Una de las leguminosas nativas de la región Puna y Janca de nuestro país es Lupinus alopecuroides Desr, esta leguminosa posee rol importante como planta pionera en colonizar espacios de suelos poco evolucionados y una vez establecida contribuye a regenerar la productividad natural de las chacras mediante la fijación biológica del N. Asimismo, los campesinos lo emplean en diversas actividades, en la salud de las personas y animales y para generar energía calorífica (leña). Dada su importancia se planteó analizar el endosimbionte de nódulos radicales de L. alopecuroides Desr desarrolladas en las chacras de Choccra Pucro (3996 m.s.n.m.), Castrovireyna, Huancavelica. Se evaluó 60 nódulos procedente de 20 plantas, se tomaron 3 nódulos por planta utilizando muestreo simple-aleatorio y se cultivaron en medio YEM. Lo que permitió obtener 30 aislados, cuando se amplificaron y secuenciaron el gen 16S rRNA se reagruparon en 14 géneros, 03 de ellos pertenecen a las rizobiaceas (Rhizobium sp., Bradyrhizobium sp. y Phyllobacterium sp.), y una vez inoculadas empleando plántulas de L. alopecuroides, el género Bradyrhizobium logró formar protuberancias de nódulos radicales. Asimismo, en los nódulos viven otros géneros no rizobianos como Erwinia, Rahnella, Serratia, Rhodococcus, Arthrobacter, Paenibacillus, Pseudomonas, Stenotrophomonas, Bacillus, Staphylococcus y Pantoea, cuya función queda por aclarar.


Conjecturas ◽  
2021 ◽  
Vol 21 (6) ◽  
pp. 88-98
Author(s):  
Delineide Pereira Gomes ◽  
Thiago Palhares Farias ◽  
Vanessa Cristina Macêdo Reis ◽  
Luciene Ferreira dos Santos ◽  
Érica Garcia França ◽  
...  
Keyword(s):  

Apesar dos resultados promissores dos inoculantes no feijão caupi, diversos fatores influenciam a eficiência dessa biotecnologia, tais como formulações inadequadas e condições de inoculação. Além disso, o tratamento com fungicidas associado aos inoculantes pode afetar a sobrevivência do rizóbio nas sementes. Sendo assim, o objetivo geral foi avaliar a sobrevivência de um rizóbio em sementes de caupi tratadas com o fungicida carbendazim e com inoculantes líquidos formulados com os polímeros goma xantana e carboximetilcelulose (CMC). Um lote de sementes de caupi da cultivar BRS Aracê foi inoculado com a estirpe UFLA 03-84, eficiente na simbiose com caupi (Bradyrhizobium sp.). Essa estirpe foi formulada com auxilio dos polímeros goma Xantana e CMC. Os polímeros foram formulados em diferentes concentrações. Uma vez obtidas as formulações, essas foram associadas ao fungicida a base de carbendazim, na dose recomendada. A sobrevivência da estirpe, em cada formulação, foi avaliada aos 0, 1, 2, 3, 4, 12, 24, 96, 192 e 768 horas de armazenamento das sementes tratadas. Os polímeros foram formulados em dez concentrações, em três repetições. O número de células viáveis foi determinado através da contagem de unidades formadoras de colônias (UFC), usando o método de diluições seriadas. A goma xantana nas concentrações de 0.025 a 1.0 g/L proporcionou, para a maioria dos períodos de armazenamento das sementes tratadas, bons valores quanto a UFC. Com relação ao CMC, concentrações a partir de 1.25 g/L de CMC proporcionaram bons valores de UFC, em vários períodos de armazenamento das sementes tratadas. Em geral, com o uso dos polímeros não se obteve um padrão de crescimento no número de células viáveis da estirpe avaliada, para as concentrações e períodos de armazenamento das sementes tratadas nas condições do estudo.


2021 ◽  
Author(s):  
L. Tighilt ◽  
F. Boulila ◽  
B. F. S. De Sousa ◽  
E. Giraud ◽  
T. Ruiz-Argüeso ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nico Nouwen ◽  
Clémence Chaintreuil ◽  
Joel Fardoux ◽  
Eric Giraud

AbstractThe Bradyrhizobium sp. strain ORS285 is able to establish a nitrogen-fixing symbiosis with both Nod factor (NF) dependent and NF-independent Aeschynomene species. Here, we have studied the growth characteristics and symbiotic interaction of a glutamate synthase (GOGAT; gltD::Tn5) mutant of Bradyrhizobium ORS285. We show that the ORS285 gltD::Tn5 mutant is unable to use ammonium, nitrate and many amino acids as nitrogen source for growth and is unable to fix nitrogen under free-living conditions. Moreover, on several nitrogen sources, the growth rate of the gltB::Tn5 mutant was faster and/or the production of the carotenoid spirilloxanthin was much higher as compared to the wild-type strain. The absence of GOGAT activity has a drastic impact on the symbiotic interaction with NF-independent Aeschynomene species. With these species, inoculation with the ORS285 gltD::Tn5 mutant does not result in the formation of nodules. In contrast, the ORS285 gltD::Tn5 mutant is capable to induce nodules on NF-dependent Aeschynomene species, but these nodules were ineffective for nitrogen fixation. Interestingly, in NF-dependent and NF-independent Aeschynomene species inoculation with the ORS285 gltD::Tn5 mutant results in browning of the plant tissue at the site of the infection suggesting that the mutant bacteria induce plant defence responses.


2021 ◽  
Author(s):  
Pongdet Piromyou ◽  
Pongpan Songwattana ◽  
Pakpoom Boonchuen ◽  
Hien P. Nguyen ◽  
Monchai Manassila ◽  
...  

Abstract Bradyrhizobium sp. DOA9 can nodulate a wide spectrum of legumes; however, unlike other bradyrhizobia, DOA9 carries a symbiotic plasmid harboring type III secretion system (T3SS) and several effector (T3E) genes, one of which encodes a new putative type III effector—SkP48. Here, we demonstrated the pivotal roles of SkP48 from Bradyrhizobium sp. DOA9 in inhibiting nodulation of various Vigna species and Crotalaria juncea and suppressing nodulation efficiency of Arachis hypogea. By contrast, the nodulation efficiency of a SkP48 mutant did not differ significantly with the DOA9 wild-type strain on Macroptilium atropurpureum and Stylosanthes hamata. An evolutionary analysis revealed that the SkP48 effector which contains a shikimate kinase and a SUMO protease (C48 cysteine peptidase) domain is distinct from the others effectors previously identified in others bradyrhizobia and pathogenic bacteria. Our findings suggest that the new putative T3E SkP48 is a key factor suppressing nodulation and nodule organogenesis in several legumes by activation of effector-triggered immunity through salicylic acid biosynthesis induction, which is deleterious to rhizobial infection. In addition, nodulation may be modulated by the function of defensins involved in jasmonic acid signalling in V. radiata SUT1.


2021 ◽  
Author(s):  
Jean-Baptiste Floc'h ◽  
Chantal Hamel ◽  
Mario Laterriere ◽  
Breanne Tidemann ◽  
Marc St-Arnaud ◽  
...  

Abstract The subterranean microbiota of plants are of great importance for plant growth and health, as root-associated microbes can perform crucial ecological functions. As the microbial environment of roots is extremely diverse, identifying keystone microorganisms in plant roots, rhizosphere and bulk soil is a necessary step towards understanding the network of influence within the microbial community associated with roots and enhancing its beneficial elements. To target these hot spots of microbial interaction, we used inter-kingdom network analysis on the canola growth phase of a long-term cropping system diversification experiment conducted at four locations in the Canadian prairies. Our aims were: to verify whether bacterial and fungal communities of canola roots, rhizosphere and bulk soil are related and influenced by diversification of the crop rotation system; to determine whether there are common or specific core fungi and bacteria in the roots, rhizosphere, and bulk soil under canola grown in different environments and with different levels of cropping system diversification; and to identify hub taxa at the inter-kingdom level that could play an important ecological role in the microbiota of canola. Our results showed that fungi were influenced by crop diversification but not by bacteria. We found no core microbiota in canola roots but identified three core fungi in the rhizosphere, one core mycobiota in the bulk soil and one core bacteria shared by the rhizosphere and bulk soil. We identified two bacterial and one fungal hub taxa in the inter-kingdom networks of the canola rhizosphere, and one bacterial and two fungal hub taxa in the bulk soil. Among these inter-kingdom hub taxa, Bradyrhizobium sp. and Mortierella sp. are particularly influential on the microbial community and the plant. To our knowledge, this is the first inter-kingdom network analysis utilized to identify hot spots of interaction in canola microbial communities.


2021 ◽  
Author(s):  
Tighilt L. ◽  
Boulila F. ◽  
De Sousa BFS ◽  
Giraud E ◽  
Ruiz-Argüeso T ◽  
...  

Abstract Many bacteria of the genus Bradyrhizobium are capable of inducing nodules in legumes. In this work, the importance of a type VI secretion system (T6SS) in a symbiotic strain of the genus Bradyrhizobium is described. T6SS of Bradyrhizobium sp. LmicA16 (A16) is necessary for efficient nodulation with Lupinus micranthus and L. angustifolius . A mutant in the gene vgrG, coding for a component of the T6SS nanostructure, induced less nodules and smaller plants than the wild type strain (wt) and was less competitive when co-inoculated with the wt strain. A16 T6SS genes are organized in a 26 kb DNA region in two divergent gene clusters of nine genes each. One of these genes codes for a protein (Tsb1) of unknown function but containing a methyltransferase domain. A tsb1 mutant showed an intermediate symbiotic phenotype regarding vgrG mutant and higher mucoidy and motility than the wt strain in free living conditions. T6SS promoter fusions to the lacZ reporter indicate expression in nodules but not in free living cells grown in different media and conditions. The analysis of nodule structure revealed that the level of nodule colonization was significantly reduced in the mutants with respect to the wt strain.


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