High-Speed Liquid Chromatography of Proteins

1977 ◽  
pp. 1-48 ◽  
Author(s):  
Fred E. Regnier ◽  
Karen M. Gooding ◽  
Shung-Ho Chang
Keyword(s):  
Liquid Chromatography ◽  
High Speed

One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

2019 ◽  
Vol 9 (2) ◽  
pp. 138-143
Author(s):  
Tianyun Li ◽  
Xiling Dai ◽  
Yichen Li ◽  
Guozheng Huang ◽  
Jianguo Cao
Keyword(s):  
Natural Products ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Speed ◽  
High Efficiency ◽  
Total Phenolic ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
One Step ◽  
Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

High-speed microbore liquid chromatography with electrochemical detection using 3-.mu.m C18 packing material

1985 ◽  
Vol 57 (12) ◽  
pp. 2423-2425 ◽  
Author(s):  
Edward J. Caliguri ◽  
Peter. Capella ◽  
Leo. Bottari ◽  
Ivan N. Mefford
Keyword(s):  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Speed ◽  
Packing Material ◽  
Microbore Liquid Chromatography

Very-high-speed liquid chromatography

1981 ◽  
Vol 217 ◽  
pp. 369-386 ◽  
Author(s):  
Joseph L. DiCesare ◽  
Michael W. Dong ◽  
John G. Atwood
Keyword(s):  
Liquid Chromatography ◽  
High Speed ◽  
Very High

Quantitative Determination of Thebaine in Poppy Plants Using High Speed Liquid Chromatography

1973 ◽  
Vol 62 (10) ◽  
pp. 1691-1694 ◽  
Author(s):  
David W. Smith ◽  
Thomas H. Beasley ◽  
Richard L. Charles ◽  
Howard W. Ziegler
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Determination ◽  
High Speed

scholarly journals Separation Analysis of Acidic Components in Human Urine by High-speed Liquid Chromatography

1979 ◽  
Vol 99 (3) ◽  
pp. 319-322 ◽  
Author(s):  
YOSHINOBU AKADA ◽  
SADAKO KAWANO ◽  
YAICHIRO TANASE
Keyword(s):  
Liquid Chromatography ◽  
Human Urine ◽  
High Speed ◽  
Acidic Components
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