scholarly journals Murine Coronavirus 5′-End Genomic RNA Sequence Reveals Mechanism of Leader-Primed Transcription

1987 ◽  
pp. 73-81 ◽  
Author(s):  
Lisa H. Soe ◽  
Chien-Kou Shieh ◽  
Shinji Makino ◽  
Ming-Fu Chang ◽  
Stephen A. Stohlman ◽  
...  
Keyword(s):  
Genomic Rna ◽  
Rna Sequence ◽  
Murine Coronavirus

scholarly journals Genomic RNA sequence of feline coronavirus strain FCoV C1Je

2007 ◽  
Vol 9 (3) ◽  
pp. 202-213 ◽  
Author(s):  
Charlotte Dye ◽  
Stuart G. Siddell
Keyword(s):  
Consensus Sequence ◽  
Pcr Amplification ◽  
Laboratory Strain ◽  
Open Reading Frames ◽  
Viral Rna ◽  
Genomic Rna ◽  
Rt Pcr ◽  
Rna Sequences ◽  
Rna Sequence ◽  
Feline Coronavirus

This paper reports the first genomic RNA sequence of a field strain feline coronavirus (FCoV). Viral RNA was isolated at post mortem from the jejunum and liver of a cat with feline infectious peritonitis (FIP). A consensus sequence of the jejunum-derived genomic RNA (FCoV C1Je) was determined from overlapping cDNA fragments produced by reverse transcriptase polymerase chain reaction (RT-PCR) amplification. RT-PCR products were sequenced by a reiterative sequencing strategy and the genomic RNA termini were determined using a rapid amplification of cDNA ends PCR strategy. The FCoV C1Je genome was found to be 29,255 nucleotides in length, excluding the poly(A) tail. Comparison of the FCoV C1Je genomic RNA sequence with that of the laboratory strain FCoV FIP virus (FIPV) 79-1146 showed that both viruses have a similar genome organisation and predictions made for the open reading frames and cis-acting elements of the FIPV 79-1146 genome hold true for FCoV C1Je. In addition, the sequence of the 3′-proximal third of the liver derived genomic RNA (FCoV C1Li), which encompasses the structural and accessory protein genes of the virus, was also determined. Comparisons of the enteric (jejunum) and non-enteric (liver) derived viral RNA sequences revealed 100% nucleotide identity, a finding that questions the well accepted ‘internal mutation theory’ of FIPV pathogenicity.

scholarly journals trans-Acting Inhibition of Genomic RNA Dimerization by Rous Sarcoma Virus Matrix Mutants

2001 ◽  
Vol 75 (1) ◽  
pp. 260-268 ◽  
Author(s):  
Rachel A. Garbitt ◽  
Jessica A. Albert ◽  
Michelle D. Kessler ◽  
Leslie J. Parent
Keyword(s):  
Rous Sarcoma Virus ◽  
Single Amino Acid ◽  
Genomic Rna ◽  
Wild Type ◽  
Protein Fusion ◽  
Dimer Formation ◽  
Rna Sequence ◽  
Rna Dimerization ◽  
Rous Sarcoma ◽  
Sarcoma Virus

ABSTRACT The genomic RNA of retroviruses exists within the virion as a noncovalently linked dimer. Previously, we identified a mutant of the viral matrix (MA) protein of Rous sarcoma virus that disrupts viral RNA dimerization. This mutant, Myr1E, is modified at the N terminus of MA by the addition of 10 amino acids from the Src protein, resulting in the production of particles containing monomeric RNA. Dimerization is reestablished by a single amino acid substitution that abolishes myristylation (Myr1E−). To distinguish between cis andtrans effects involving Myr1E, additional mutations were generated. In Myr1E.cc and Myr1E−.cc, different nucleotides were utilized to encode the same protein as Myr1E and Myr1E−, respectively. The alterations in RNA sequence did not change the properties of the viral mutants. Myr1E.ATG− was constructed so that translation began at the gag AUG, resulting in synthesis of the wild-type Gag protein but maintenance of the src RNA sequence. This mutant had normal infectivity and dimeric RNA, indicating that thesrc sequence did not prevent dimer formation. All of the src-containing RNA sequences formed dimers in vitro. Examination of MA-green fluorescent protein fusion proteins revealed that the wild-type and mutant MA proteins Myr1E.ATG−, Myr1E−, and Myr1E−.cc had distinctly different patterns of subcellular localization compared with Myr1E and Myr1E.cc MA proteins. This finding suggests that proper localization of the MA protein may be required for RNA dimer formation and infectivity. Taken together, these results provide compelling evidence that the genomic RNA dimerization defect is due to a trans-acting effect of the mutant MA proteins.

scholarly journals First Complete Genome Sequence of Suakwa aphid-borne yellows virus from East Timor

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Solomon Maina ◽  
Owain R. Edwards ◽  
Luis de Almeida ◽  
Abel Ximenes ◽  
Roger A. C. Jones
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
East Timor ◽  
Nucleotide Identity ◽  
Genomic Rna ◽  
Rna Sequence ◽  
Complete Genomic

We present here the first complete genomic RNA sequence of the polerovirus Suakwa aphid-borne yellows virus (SABYV), from East Timor. The isolate sequenced came from a virus-infected pumpkin plant. The East Timorese genome had a nucleotide identity of 86.5% with the only other SABYV genome available, which is from Taiwan.

scholarly journals Genomic RNA of the Murine Coronavirus JHM

1978 ◽  
Vol 41 (2) ◽  
pp. 217-227 ◽  
Author(s):  
H. Wege ◽  
A. Muller ◽  
V. Ter Meulen
Keyword(s):  
Genomic Rna ◽  
Murine Coronavirus

Complete genomic RNA sequence of the Polish Pepino mosaic virus isolate belonging to the US2 strain

Virus Genes ◽  
2007 ◽  
Vol 36 (1) ◽  
pp. 209-214 ◽  
Author(s):  
Beata Hasiów ◽  
Natasza Borodynko ◽  
Henryk Pospieszny
Keyword(s):  
Mosaic Virus ◽  
Virus Isolate ◽  
Genomic Rna ◽  
Pepino Mosaic Virus ◽  
Rna Sequence ◽  
Complete Genomic

scholarly journals First Complete Genome Sequence of Pepper vein yellows virus from Australia

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Solomon Maina ◽  
Owain R. Edwards ◽  
Roger A. C. Jones
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Pepper Plant ◽  
Genomic Rna ◽  
Rna Sequence ◽  
Pepper Vein Yellows Virus ◽  
Japan And China ◽  
Complete Genomic

We present here the first complete genomic RNA sequence of the polerovirus Pepper vein yellows virus (PeVYV) obtained from a pepper plant in Australia. We compare it with complete PeVYV genomes from Japan and China. The Australian genome was more closely related to the Japanese than the Chinese genome.

scholarly journals Cloning and Characterization of the Genomic RNA Sequence of the Mumps Virus Strain Associated with a High Incidence of Aseptic Meningitis

1998 ◽  
Vol 42 (2) ◽  
pp. 133-137 ◽  
Author(s):  
Hiroyuki Saito ◽  
Yoshihiro Takahashi ◽  
Seizaburo Harata ◽  
Keiko Tanaka ◽  
Hiroyasu Sato ◽  
...  
Keyword(s):  
Aseptic Meningitis ◽  
Virus Strain ◽  
Mumps Virus ◽  
Genomic Rna ◽  
Rna Sequence ◽  
High Incidence
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