The High-Affinity (Ca2+ —Mg2+ )ATPase of Rat Liver Plasma Membrane Hydrolyzes Extracellular ATP

1989 ◽  
pp. 13-19 ◽  
Author(s):  
Sue-Hwa Lin
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Extracellular Atp ◽  
Liver Plasma Membrane ◽  
High Affinity

scholarly journals The effect of thyroid hormone on the high affinity Ca2+-ATPase in rat liver plasma membrane.

1984 ◽  
Vol 31 (2) ◽  
pp. 141-149 ◽  
Author(s):  
KAZUTAKA HARAGUCHI ◽  
KIYOSHI HASHIZUME ◽  
KAZUO ICHIKAWA ◽  
MUTSUHIRO KOBAYASHI
Keyword(s):  
Plasma Membrane ◽  
Thyroid Hormone ◽  
Rat Liver ◽  
Liver Plasma Membrane ◽  
High Affinity

scholarly journals ATP-dependent bile-salt transport in canalicular rat liver plasma-membrane vesicles

1992 ◽  
Vol 284 (1) ◽  
pp. 67-74 ◽  
Author(s):  
B Stieger ◽  
B O'Neill ◽  
P J Meier
Keyword(s):  
Plasma Membrane ◽  
Bile Salt ◽  
Rat Liver ◽  
Transport System ◽  
Membrane Vesicles ◽  
Salt Transport ◽  
Inhibitory Effects ◽  
Liver Plasma Membrane ◽  
High Affinity ◽  
Bile Salt Transport

The present study identifies and characterizes a novel ATP-dependent bile-salt transport system in isolated canalicular rat liver plasma-membrane (cLPM) vesicles. ATP (1-5 mM) stimulated taurocholate uptake into cLPM vesicles between 6- and 8-fold above equilibrium uptake values (overshoot) and above values for incubations in the absence of ATP. The ATP-dependent portion of taurocholate uptake was 2-fold higher in the presence of equilibrated KNO3 as compared with potassium gluconate, indicating that the stimulatory effect of ATP was not due to the generation of an intravesicular positive membrane potential. Saturation kinetics revealed a very high affinity (Km approximately 2.1 microM) of the system for taurocholate. The system could only minimally be stimulated by nucleotides other than ATP. Furthermore, it was preferentially inhibited by conjugated univalent bile salts. Further strong inhibitory effects were observed with valinomycin, oligomycin, 4,4′-di-isothiocyano-2,2′-stilbene disulphonate, sulphobromophthalein, leukotriene C4 and N-ethylmaleimide, whereas nigericin, vanadate, GSH, GSSG and daunomycin exerted only weak inhibitory effects or none at all. These results indicate the presence of a high-affinity primary ATP-dependent bile-salt transport system in cLPM vesicles. This transport system might be regulated in vivo by the number of carriers present at the perspective transport site(s), which, in addition to the canalicular membrane, might also include pericanalicular membrane vesicles.

scholarly journals Glucagon-(19-29), a Ca2+ pump inhibitory peptide, is processed from glucagon in the rat liver plasma membrane by a thiol endopeptidase.

1990 ◽  
Vol 265 (35) ◽  
pp. 21514-21519 ◽  
Author(s):  
P Blache ◽  
A Kervran ◽  
M Dufour ◽  
J Martinez ◽  
D Le-Nguyen ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Inhibitory Peptide ◽  
Liver Plasma Membrane

Age-related changes in rat liver plasma membrane sphingomyelinase activity

1988 ◽  
Vol 23 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Diana H. Petkova ◽  
Albena B. Momchilova-Pankova ◽  
Tania T. Markovska ◽  
Kamen S. Koumanov
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Liver Plasma Membrane ◽  
Age Related ◽  
Age Related Changes
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