The peat stratigraphy of fens at Muskiki Lake (52°50′N, 116°51′W) and Marguerite Lake (54°38′N, 110°43′W) in central Alberta was examined to determine the developmental history of the fens on the basis of radiocarbon dating and bryophyte macrofossil analyses. Peat accumulation at Muskiki Lake peatland began about 9000 years BP via lake filling. Expansion of the peatland by paludification and vegetation changes, including string and flark formation, are secondary occurrences, the latter occurring subsequent to environmental changes, including mid-Holocene thermal events. Peat inception at Marguerite Lake began about 2400 years BP via paludification. This delay, relative to Muskiki Lake peatland, may be related to the mid-Holocene warm period. Fire activity and increased wetness, as well as autogenic successional processes, altered species composition and abundance.