Genetic and Structural Studies of a V-Region Marker in Mouse Immunoglobulin Light Chains

1976 ◽  
pp. 185-216 ◽  
Author(s):  
Paul D. Gottlieb
Keyword(s):  
Light Chains ◽  
Structural Studies ◽  
Immunoglobulin Light Chains ◽  
Mouse Immunoglobulin ◽  
V Region

Translation of messenger RNA for mouse immunoglobulin light chains in living frog oocytes

1973 ◽  
Vol 80 (3) ◽  
pp. 553-557 ◽  
Author(s):  
M. Smith ◽  
J. Stavnezer ◽  
R.C. Huang ◽  
J.B. Gurdon ◽  
C.D. Lane
Keyword(s):  
Messenger Rna ◽  
Light Chains ◽  
Immunoglobulin Light Chains ◽  
Mouse Immunoglobulin

scholarly journals Sequence diversity within a subgroup of mouse immunoglobulin kappa chains controlled by the IgK-Ef2 locus.

1981 ◽  
Vol 154 (1) ◽  
pp. 146-155 ◽  
Author(s):  
C Lazure ◽  
W T Hum ◽  
D M Gibson
Keyword(s):  
Normal Mouse ◽  
Sequence Diversity ◽  
The Other ◽  
Light Chains ◽  
Chromosome 6 ◽  
Immunoglobulin Kappa ◽  
Mouse Immunoglobulin ◽  
Complete Sequencing ◽  
Polymorphic Bands ◽  
V Region

We previously showed that a chromosome 6 locus, IgK-Ef2, controls a pair of prominent bands in normal mouse light-chain isoelectric focusing profiles. Screening of myeloma light chains derived from BALB/c mice (an IgK-EF2 alpha strain) led to the identification of seven light chains cofocusing with the polymorphic bands controlled by IgK-Ef2. Complete sequencing of the variable (V) regions of four of the light chains indicates that they are all members of the same subgroup (Vk-1A) and they differ from one another by 1--3 substitutions. One of the protein differs from the prototype V-region sequence only in the deletion of a single residue at position 95 immediately preceding of J region. The other two differ from the protype V region by 3 (two framework [fr], one complementarity-determined [cdr]) and one (fr) residues, respectively. Complete V-region sequences of two closely related light chains derived from NZB mice (an IgK-Ef2b strain) indicate the NZB proteins are derived from a distinct Vk gene (Vk-1B), differing by four substitutions from the Vk-1A sequence. The results suggest that the IgK-Ef2 polymorphism may be a result of, at least in part, the loss of the gene(s) coding for the Vk-1A subgroups in IgK-Ef2b strains of mice. The nature of the sequence diversity found in the Vk-1A subgroup indicates that either it is coded by a repeated series of virtually identical genes or that somatic mutation of a single Vk-1A gene may give rise to substitutions in framework as well as cdr regions.

scholarly journals Genetic polymorphism of mouse immunoglobulin light chains revealed by isoelectric focusing.

1976 ◽  
Vol 144 (1) ◽  
pp. 298-303 ◽  
Author(s):  
D Gibson
Keyword(s):  
Isoelectric Focusing ◽  
Inbred Mouse ◽  
Rapid Analysis ◽  
Light Chains ◽  
Mouse Strains ◽  
Immunoglobulin Light Chains ◽  
F1 Hybrid ◽  
Mouse Immunoglobulin ◽  
Gel Isoelectric Focusing ◽  
Hybrid Mice

Light chains isolated from normal immunoglobulin of unimmunized mice were analyzed by gel isoelectric focusing. Examination of the focusing patterns of light chains from nine inbred mouse strains showed that six of the strains (SWR/J, C3H/HeJ, DBA/1J, A/J, CBA/J, and C57BL/6J) possessed a virtually identical spectrum of focusing bands, while the remaining three strains (RF/J, AKR/J, and C58/J) showed clear differences involving several bands. Analysis of the light chains of individual SWR/J, C58/J, and F1 hybrid mice indicated that the differences in focusing pattern were inherited in a simple codominant fashion. A new procedure was developed for the rapid analysis of light chains from small quantities of serum.

scholarly journals GENETIC CORRELATION OF A MOUSE LIGHT CHAIN VARIABLE REGION MARKER WITH A THYMOCYTE SURFACE ANTIGEN

1974 ◽  
Vol 140 (5) ◽  
pp. 1432-1437 ◽  
Author(s):  
Paul D. Gottlieb
Keyword(s):  
Linkage Group ◽  
Surface Antigen ◽  
Strain Distribution ◽  
Congenic Strain ◽  
Variable Region ◽  
Light Chains ◽  
Immunoglobulin Light Chains ◽  
Mouse Immunoglobulin ◽  
Peptide Expression ◽  
Peptide Marker

The inbred and congenic strain distribution of the IH-peptide marker in the variable region of mouse immunoglobulin light chains has been compared with other known genetic markers. A positive correlation was noted between the IH-peptide marker and expression of the Ly-3.1 thymocyte cell surface antigen. This suggests that the locus responsible for IH-peptide expression is genetically linked to the Ly-2 and Ly-3 loci in linkage group XI on chromsome 6 of the mouse.

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