Regulation of Peroxisomal Fatty Acyl-CoA Oxidase in the Yeast

1997 ◽  
pp. 157-166 ◽  
Author(s):  
Gillian M. Small ◽  
Igor V. Karpichev ◽  
Yi Luo
Keyword(s):  
Fatty Acyl ◽  
Acyl Coa Oxidase

scholarly journals Identification and purification of a peroxisomal branched chain fatty acyl-CoA oxidase.

1991 ◽  
Vol 266 (36) ◽  
pp. 24676-24683
Author(s):  
P.P. Van Veldhoven ◽  
G. Vanhove ◽  
F. Vanhoutte ◽  
G. Dacremont ◽  
G. Parmentier ◽  
...  
Keyword(s):  
Fatty Acyl ◽  
Acyl Coa Oxidase ◽  
Branched Chain

scholarly journals Acyl-CoA synthetase and the peroxisomal enzymes of β-oxidation in human liver. Quantitative analysis of their subcellular localization

1984 ◽  
Vol 224 (3) ◽  
pp. 709-720 ◽  
Author(s):  
M Bronfman ◽  
N C Inestrosa ◽  
F O Nervi ◽  
F Leighton
Keyword(s):  
Human Liver ◽  
Fatty Acyl ◽  
Single Step ◽  
Synthetase Activity ◽  
Beta Oxidation ◽  
Analysis Of Results ◽  
Fractionation Method ◽  
Hydroxyacyl Coa Dehydrogenase ◽  
Acyl Coa Oxidase ◽  
Rate Limiting

The presence of acyl-CoA synthetase (EC 6.2.1.3) in peroxisomes and the subcellular distribution of beta-oxidation enzymes in human liver were investigated by using a single-step fractionation method of whole liver homogenates in metrizamide continuous density gradients and a novel procedure of computer analysis of results. Peroxisomes were found to contain 16% of the liver palmitoyl-CoA synthetase activity, and 21% and 60% of the enzyme activity was localized in mitochondria and microsomal fractions respectively. Fatty acyl-CoA oxidase was localized exclusively in peroxisomes, confirming previous results. Human liver peroxisomes were found to contribute 13%, 17% and 11% of the liver activities of crotonase, beta-hydroxyacyl-CoA dehydrogenase and thiolase respectively. The absolute activities found in peroxisomes for the enzymes investigated suggest that in human liver fatty acyl-CoA oxidase is the rate-limiting enzyme of the peroxisomal beta-oxidation pathway, when palmitic acid is the substrate.

Peroxisomal fatty acyl-CoA oxidase is not regulated by triiodothyronine

1990 ◽  
Vol 1044 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Randall L. Shoemaker ◽  
Russell K. Yamazaki
Keyword(s):  
Fatty Acyl ◽  
Acyl Coa Oxidase

Differential induction of peroxisomal β-oxidation enzymes by clofibric acid and aspirin in piglet tissues

2001 ◽  
Vol 281 (5) ◽  
pp. R1553-R1561 ◽  
Author(s):  
Xing Xian Yu ◽  
Jack Odle ◽  
James K. Drackley
Keyword(s):  
Body Weight ◽  
Liver Weight ◽  
Fatty Acyl ◽  
Clofibric Acid ◽  
Heart Weight ◽  
Final Body Weight ◽  
Daily Food ◽  
Newborn Pigs ◽  
Acyl Coa Oxidase ◽  
Total Carnitine

Peroxisomal β-oxidation (POX) of fatty acids is important in lipid catabolism and thermogenesis. To investigate the effects of peroxisome proliferators on peroxisomal and mitochondrial β-oxidation in piglet tissues, newborn pigs (1–2 days old) were allowed ad libitum access to milk replacer supplemented with 0.5% clofibric acid (CA) or 1% aspirin for 14 days. CA increased ratios of liver weight to body weight ( P < 0.07), kidney weight to body weight ( P< 0.05), and heart weight to body weight ( P < 0.001). Aspirin decreased daily food intake and final body weight but increased the ratio of heart weight to body weight ( P < 0.01). In liver, activities of POX, fatty acyl-CoA oxidase (FAO), total carnitine palmitoyltransferase (CPT), and catalase were 2.7-, 2.2-, 1.5-fold, and 33% greater, respectively, for pigs given CA than for control pigs. In heart, these variables were 2.2-, 4.1-, 1.9-, and 1.8-fold greater, respectively, for pigs given CA than for control pigs. CA did not change these variables in either kidney or muscle, except that CPT activity was increased ∼110% ( P < 0.01) in kidney. Aspirin increased only hepatic FAO and CPT activities. Northern blot analysis revealed that CA increased the abundance of catalase mRNA in heart by ∼2.2-fold. We conclude that 1) POX and CPT in newborn pigs can be induced by peroxisomal proliferators with tissue specificity and 2) the relatively smaller induction of POX in piglets (compared with that in young or adult rodents) may be related to either age or species differences.

Improvement in γ-decalactone production by Yarrowia sp. after genome shuffling

2014 ◽  
Vol 68 (8) ◽  
Author(s):  
Yu-Ping Zhao ◽  
Xiao-Qing Mu ◽  
Yan Xu
Keyword(s):  
Cell Growth ◽  
Genome Shuffling ◽  
Fermentation Medium ◽  
Fatty Acyl ◽  
Culture Collection ◽  
Fermentation Time ◽  
Mutant Strains ◽  
Protoplast Preparation ◽  
Acyl Coa Oxidase ◽  
Initial Strains

AbstractIn this study, a modified genome shuffling method was used to improve γ-decalactone (GDL) production of Yarrowia sp. China General Microbiological Culture Collection Center (CGMCC 2.1405). Five UV mutant strains with higher GDL production or shorter fermentation time were selected as the initial strains for genome shuffling. Conditions of protoplast preparation, regeneration, inactivation, fusion, sporulation of recombinant fusants and ascospore isolation were optimized. Four hereditarily stable haploid recombinants with high GDL production were obtained by three rounds of genome shuffling. Among them, a high GDL-producing recombinant, G3-3.21, producing 3.75 g L−1 of GDL in the fermentation medium after 64 h was obtained. This value is 6.54-fold higher than that of the parent strains CGMCC 2.1405, at the peak production shortened by 8 h. Mathematical kinetic models of CGMCC 2.1405 and G3-3.21 were established to well predict the cell growth and GDL production. The cell growth of G3-3.21 was significantly faster than that of CGMCC 2.1405. The product synthesis constant associated with the strain growth of G3-3.21 was higher than that associated with CGMCC 2.1405. Long-chain fatty-acyl-CoA oxidase activities of G3-3.21 were 833 mU mg−1 and 6.83 times higher than that of CGMCC 2.1405.

Stable Expression of Peroxisomal Fatty Acyl-CoA Oxidase in Vitro

1996 ◽  
Vol 804 (1 Peroxisomes) ◽  
pp. 787-791
Author(s):  
SOHEIL S. DADRAS ◽  
SNORRI S. THORGEIRSSON ◽  
JANARDAN K. REDDY
Keyword(s):  
Fatty Acyl ◽  
Stable Expression ◽  
Acyl Coa Oxidase
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