Fast Multiplex Polymerase Chain Reaction on Boiled Clinical Samples for Rapid Diagnosis of Viral Infections

1994 ◽  
pp. 87-99
Author(s):  
Christian Vandenvelde ◽  
Danièle Van Beers
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Viral Infections ◽  
Rapid Diagnosis ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Polymerase chain reaction (PCR). Description of the method and its application in the detection of viruses

2018 ◽  
Vol 48 (2) ◽  
pp. 61
Author(s):  
M. PAPANASTASSOPOULOU (Μ. ΠΑΠΑΝΑΣΤΑΣΟΠΟΥΛΟΥ)
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Infections ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Polymerase Chain

Since the first publication of the polymerase chain reaction (PCR) in 1985, there has been a large number of reports on its applications in the study of viral infections. This review outlines the PCR methodology and its variations, as well as the problems that may be encountered during its performance. Its application in the detection of various viruses in clinical samples is also presented.

Detection of enterovirus RNA in clinical samples by nested polymerase chain reaction for rapid diagnosis of enterovirus infection

1994 ◽  
Vol 48 (2-3) ◽  
pp. 155-166 ◽  
Author(s):  
Felicity Nicholson ◽  
Gheeaneshwar Meetoo ◽  
Subramania Aiyar ◽  
Jehangir E. Banatvala ◽  
Peter Muir
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapid Diagnosis ◽  
Clinical Samples ◽  
Enterovirus Infection ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Identification of Listeria spp. strains isolated from meat products and meat production plants by multiplex polymerase chain reaction

2015 ◽  
Vol 4 (4) ◽  
Author(s):  
Roberta Mazza ◽  
Francesca Piras ◽  
Daniela Ladu ◽  
Miriam Putzolu ◽  
Simonetta Gianna Consolati ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Meat Products ◽  
Clinical Samples ◽  
Meat Production ◽  
Chain Reaction ◽  
Processing Plants ◽  
Contaminated Food ◽  
Polymerase Chain

Listeriosis is a foodborne disease caused by <em>Listeria monocytogenes</em> and is considered as a serious health problem, due to the severity of symptoms and the high mortality rate. Recently, other <em>Listeria</em> species have been associated with disease in human and animals. The aim of this study was to develop a multiplex polymerase chain reaction (PCR) in order to simultaneously detect six Listeria species (<em>L. grayi</em>, <em>L. welshimeri</em>, <em>L. ivanovii</em>, <em>L. monocytogenes</em>, <em>L. seeligeri</em>, <em>L. innocua</em>) in a single reaction. One hundred eighteen <em>Listeria</em> spp. strains, isolated from meat products (sausages) and processing plants (surfaces in contact and not in contact with meat), were included in the study. All the strains were submitted to biochemical identification using the API Listeria system. A multiplex PCR was developed with the aim to identify the six species of <em>Listeria</em>. PCR allowed to uniquely identify strains that had expressed a doubtful profile with API Listeria The results suggest that the multiplex PCR could represent a rapid and sensitive screening test, a reliable method for the detection of all <em>Listeria</em> species, both in contaminated food and in clinical samples, and also a tool that could be used for epidemiological purposes in food-borne outbreaks. A further application could be the development of a PCR that can be directly applied to the pre-enrichment broth.

Detecting Gram-Positive Anaerobic Cocci Directly From the Clinical Samples by Multiplex Polymerase Chain Reaction in Odontogenic Infections

2015 ◽  
Vol 73 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Ayşegül Mine Tüzüner Öncül ◽  
Emel Uzunoğlu ◽  
Zeynep Ceren Karahan ◽  
A. Murat Aksoy ◽  
Reha Kişnişci ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Gram Positive ◽  
Odontogenic Infections ◽  
Polymerase Chain
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