The Role of the Endothelial Cell Surface Charge for Blood-Brain Barrier Function

1995 ◽  
pp. 63-70
Author(s):  
Barbro B. Johansson
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Surface Charge ◽  
Blood Brain Barrier ◽  
Barrier Function ◽  
Brain Barrier ◽  
Endothelial Cell Surface ◽  
Blood Brain ◽  
Cell Surface Charge

scholarly journals Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier

1995 ◽  
Vol 202 (4) ◽  
pp. 325-332 ◽  
Author(s):  
R. Hallmann ◽  
D. N. Mayer ◽  
E. L. Berg ◽  
R. Broermann ◽  
E. C. Butcher
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Blood Brain Barrier ◽  
Surface Marker ◽  
Cell Surface Marker ◽  
Brain Barrier ◽  
Endothelial Cell Surface ◽  
Blood Brain

scholarly journals Human brain microvascular endothelial cell pairs model tissue-level blood–brain barrier function

2020 ◽  
Vol 12 (3) ◽  
pp. 64-79
Author(s):  
Blakely B O’Connor ◽  
Thomas Grevesse ◽  
John F Zimmerman ◽  
Herdeline Ann M Ardoña ◽  
Jorge A Jimenez ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Human Brain ◽  
Blood Brain Barrier ◽  
Barrier Function ◽  
Cytoskeletal Proteins ◽  
Fiber Formation ◽  
Brain Barrier ◽  
Actin Stress Fiber ◽  
Barrier Permeability ◽  
Blood Brain

Abstract The blood–brain barrier plays a critical role in delivering oxygen and nutrients to the brain while preventing the transport of neurotoxins. Predicting the ability of potential therapeutics and neurotoxicants to modulate brain barrier function remains a challenge due to limited spatial resolution and geometric constraints offered by existing in vitro models. Using soft lithography to control the shape of microvascular tissues, we predicted blood–brain barrier permeability states based on structural changes in human brain endothelial cells. We quantified morphological differences in nuclear, junction, and cytoskeletal proteins that influence, or indicate, barrier permeability. We established a correlation between brain endothelial cell pair structure and permeability by treating cell pairs and tissues with known cytoskeleton-modulating agents, including a Rho activator, a Rho inhibitor, and a cyclic adenosine monophosphate analog. Using this approach, we found that high-permeability cell pairs showed nuclear elongation, loss of junction proteins, and increased actin stress fiber formation, which were indicative of increased contractility. We measured traction forces generated by high- and low-permeability pairs, finding that higher stress at the intercellular junction contributes to barrier leakiness. We further tested the applicability of this platform to predict modulations in brain endothelial permeability by exposing cell pairs to engineered nanomaterials, including gold, silver–silica, and cerium oxide nanoparticles, thereby uncovering new insights into the mechanism of nanoparticle-mediated barrier disruption. Overall, we confirm the utility of this platform to assess the multiscale impact of pharmacological agents or environmental toxicants on blood–brain barrier integrity.

scholarly journals The blood- brain barrier and barrier function in vivo: the role of tight junctions

2013 ◽  
Vol 28 (4) ◽  
pp. 279-286
Author(s):  
Noriko Iwamoto ◽  
Mikio Furuse
Keyword(s):  
Tight Junctions ◽  
Blood Brain Barrier ◽  
Barrier Function ◽  
Brain Barrier ◽  
Blood Brain

The Role of Pericytes in Blood-Brain Barrier Function and Stroke

2012 ◽  
Vol 18 (25) ◽  
pp. 3653-3662 ◽  
Author(s):  
Shuo Liu ◽  
Dritan Agalliu ◽  
Chuanhui Yu ◽  
Mark Fisher
Keyword(s):  
Blood Brain Barrier ◽  
Barrier Function ◽  
Brain Barrier ◽  
Blood Brain

scholarly journals Human iPSC-derived blood-brain barrier microvessels: validation of barrier function and endothelial cell behavior

Biomaterials ◽  
2019 ◽  
Vol 190-191 ◽  
pp. 24-37 ◽  
Author(s):  
Raleigh M. Linville ◽  
Jackson G. DeStefano ◽  
Matt B. Sklar ◽  
Zinnia Xu ◽  
Alanna M. Farrell ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Barrier Function ◽  
Brain Barrier ◽  
Cell Behavior ◽  
Blood Brain ◽  
Human Ipsc
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