Enzymatic Determination of Cholic Acids in Human Bile by Amperometric Oxygen Electrode as Detector

1986 ◽  
pp. 543-548 ◽  
Author(s):  
L. Campanella ◽  
F. Bartoli ◽  
R. Morabito ◽  
M. Tomassetti
Keyword(s):  
Oxygen Electrode ◽  
Human Bile ◽  
Enzymatic Determination ◽  
Cholic Acids

Enzymatic Determination of Hydroxysteroids in Human Skin Surface Lipids1

1963 ◽  
Vol 41 (5) ◽  
pp. 265-268 ◽  
Author(s):  
Thomas J Cook ◽  
Allan L Lorincz ◽  
Alan R Spector
Keyword(s):  
Human Skin ◽  
Skin Surface ◽  
Enzymatic Determination

Determination of aeration capacity of bubbled column type fermentors with oxygen electrode

1978 ◽  
Vol 43 (3) ◽  
pp. 713-718 ◽  
Author(s):  
Jaroslav Votruba ◽  
Miroslav Sobotka ◽  
Aleš Prokop
Keyword(s):  
Oxygen Electrode ◽  
Column Type ◽  
Aeration Capacity

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

1983 ◽  
Vol 29 (8) ◽  
pp. 1513-1517 ◽  
Author(s):  
M W McGowan ◽  
J D Artiss ◽  
B Zak
Keyword(s):  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Amniotic Fluid ◽  
Enzymatic Reaction ◽  
Detergent Solution ◽  
Enzymatic Determination ◽  
Red Color ◽  
Hydrolysis Of ◽  
Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

Non-enzymatic determination of purine nucleotides using a carbon dot modified glassy carbon electrode

2019 ◽  
Vol 11 (30) ◽  
pp. 3866-3873 ◽  
Author(s):  
R. Karthikeyan ◽  
D. James Nelson ◽  
S. Abraham John
Keyword(s):  
Glassy Carbon ◽  
Low Cost ◽  
Phosphate Buffer Solution ◽  
Purine Nucleotides ◽  
Buffer Solution ◽  
Solution Ph ◽  
Carbon Dot ◽  
Enzymatic Determination ◽  
Sensitive Determination

Selective and sensitive determination of one of the purine nucleotides, inosine (INO) using a low cost carbon dot (CD) modified glassy carbon (GC) electrode in 0.2 M phosphate buffer solution (pH 7.2) was demonstrated in this paper.

scholarly journals A simple method for the determination of lipid composition of human bile.

1991 ◽  
Vol 32 (2) ◽  
pp. 371-374
Author(s):  
M Muraca ◽  
MT Vilei ◽  
L Miconi ◽  
P Petrin ◽  
M Antoniutti ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Simple Method ◽  
Human Bile
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