Spin Label Study of Molecular Aggregates

1984 ◽  
pp. 559-563
Author(s):  
M. Schara ◽  
M. Nemec
Keyword(s):  
Spin Label ◽  
Molecular Aggregates ◽  
Label Study

scholarly journals A spin label study of the thyroid hormone-binding sites in human plasma thyroxine transport proteins.

1981 ◽  
Vol 256 (2) ◽  
pp. 831-836
Author(s):  
S.Y. Cheng ◽  
G. Rakhit ◽  
F. Erard ◽  
J. Robbins ◽  
C.F. Chignell
Keyword(s):  
Thyroid Hormone ◽  
Human Plasma ◽  
Spin Label ◽  
Binding Sites ◽  
Transport Proteins ◽  
Hormone Binding ◽  
Label Study

scholarly journals A Spin-Label Study on Lipids in Dough Formed at Various Concentrations of Oxygen

1985 ◽  
Vol 49 (9) ◽  
pp. 2557-2561
Author(s):  
Junko Nishiyama ◽  
Toyo Kuninori
Keyword(s):  
Spin Label ◽  
Label Study

scholarly journals Spin-label study of hemoglobin conformations in solution.

1967 ◽  
Vol 58 (1) ◽  
pp. 19-26 ◽  
Author(s):  
S. Ogawa ◽  
H. M. McConnell
Keyword(s):  
Spin Label ◽  
Label Study

The Orientation of Cytochrome c Oxidase in Coupled Phospholipid Membrane Vesicles—A Spin-Label Study

1975 ◽  
Vol 53 (3) ◽  
pp. 364-370 ◽  
Author(s):  
J. A. Kornblatt ◽  
W. L. Chen ◽  
J. C. Hsia ◽  
G. R. Williams
Keyword(s):  
Molecular Weight ◽  
Binding Site ◽  
Cytochrome Oxidase ◽  
Spin Label ◽  
Resonance Spectrum ◽  
Membrane Vesicles ◽  
Phospholipid Membrane ◽  
Vesicle Membrane ◽  
Exterior Surface ◽  
Label Study

Cytochrome oxidase, an enzyme containing six different subunits, has been shown to span the inner mitochrondrial membrane. The arrangement of the subunits within the membrane is unknown. We have specifically labeled the 25 000 molecular weight subunit with a spin-label derivative of N-ethylmaleimide, 3-maleimido-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl (NEM-SL(5)). NEM-SL(5)-labeled cytochrome oxidase can be incorporated into phospholipid membranes to form coupled vesicles of the Hinkle, Kim &Racker ((1972) J. Biol. Chem. 247, 1338–1339) type. The resonance spectrum of NEM-SL(5) is similar in both soluble and vesicular cytochrome oxidase. Since ascorbate has been shown to reduce only spin label that is exposed to the exterior surface of a closed vesicle, we have used ascorbate to determine the NEM-SL(5)-binding site in the coupled vesicles. NEM-SL(5)-labeled cytochrome oxidase vesicles are reduced by 10 mM ascorbate with [Formula: see text] of 1 min at 22 °C. The rate of reduction is relatively independent of temperature. We conclude that (1) cytochrome oxidase is unidirectionally or preferentially oriented in the vesicle membrane, and (2) the NEM-SL(5)-binding site on the 25 000 molecular weight subunit is exposed to the external aqueous medium.

scholarly journals Changes of Membrane Fluidity in Erythrocytes of Obese Children: A Spin Label Study

1987 ◽  
Vol 22 (2) ◽  
pp. 141-144 ◽  
Author(s):  
Giovanna Curatola ◽  
Gianna Ferretti ◽  
Enrico Bertoli ◽  
Maria Dotti ◽  
Edoardo Bartolotta ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Spin Label ◽  
Obese Children ◽  
Label Study

A spin-label study of membrane proteins and internal microviscosity of erythrocytes in hereditary spherocytosis

Life Sciences ◽  
1987 ◽  
Vol 41 (20) ◽  
pp. 2285-2288 ◽  
Author(s):  
G. Bartosz ◽  
M. Gaczyńska ◽  
E. Grzelińska ◽  
L. Judkiewicz
Keyword(s):  
Membrane Proteins ◽  
Spin Label ◽  
Hereditary Spherocytosis ◽  
Label Study

Polydispersity of aggregates formed by the polyene antibiotic amphotericin B and deoxycholate. A spin label study

1989 ◽  
Vol 981 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Maria Teresa Lamy-Freund ◽  
Vergínia F.N. Ferreira ◽  
Shirley Schreier
Keyword(s):  
Amphotericin B ◽  
Spin Label ◽  
Polyene Antibiotic ◽  
Label Study

Spin Label Study of Apomembranes and Purple Membranes

1993 ◽  
Vol 48 (5-6) ◽  
pp. 500-503
Author(s):  
Tzvetana R. Lazarova ◽  
Maya Y. Velitchkova
Keyword(s):  
Fatty Acids ◽  
Lipid Bilayer ◽  
Spin Label ◽  
Hyperfine Splitting ◽  
Esr Spectra ◽  
Spin Labels ◽  
Label Study ◽  
Splitting Parameter ◽  
Induced Changes ◽  
Purple Membranes

Abstract Three spin-labelled fatty acids were used to detect the dynamics of lipid bilayer of apomem branes and purple membranes. It was found that ESR spectra of spin labels bound to apo­ membranes showed a temperature-induced changes rather similar to those seen with purple membranes. At the same time, however, the values of hyperfine splitting parameter 2Tm were lower as compared to purple membranes. The results pointed out that the removal of the retinal from purple membranes affects the dynamics of lipid bilayer and apomembranes were more rigid structure than those of purple membranes.

Vesicular stomatitis virus induced membrane changes: A spin label study

Life Sciences ◽  
1984 ◽  
Vol 35 (7) ◽  
pp. 747-752 ◽  
Author(s):  
Peter L. Guitierrez ◽  
Lydia Hernaez Davis ◽  
Raveendran Pottathil
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Spin Label ◽  
Label Study ◽  
Induced Membrane ◽  
Vesicular Stomatitis ◽  
Membrane Changes
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