Direct Reprogramming of Amniotic Cells into Endothelial Cells

2014 ◽  
pp. 67-85
Author(s):  
Koji Shido ◽  
Joseph M. Scandura ◽  
Shahin Rafii ◽  
Venkat R. Pulijaal
Keyword(s):  
Endothelial Cells ◽  
Direct Reprogramming ◽  
Amniotic Cells

scholarly journals Efficient Direct Reprogramming of Mature Amniotic Cells into Endothelial Cells by ETS Factors and TGFβ Suppression

Cell ◽  
2012 ◽  
Vol 151 (3) ◽  
pp. 559-575 ◽  
Author(s):  
Michael Ginsberg ◽  
Daylon James ◽  
Bi-Sen Ding ◽  
Daniel Nolan ◽  
Fuqiang Geng ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Direct Reprogramming ◽  
Ets Factors ◽  
Amniotic Cells

scholarly journals Significant improvement of direct reprogramming efficacy of fibroblasts into progenitor endothelial cells by ETV2 and hypoxia

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Phuc Van Pham ◽  
Ngoc Bich Vu ◽  
Hoa Trong Nguyen ◽  
Oanh Thuy Huynh ◽  
Mai Thi-Hoang Truong
Keyword(s):  
Endothelial Cells ◽  
Direct Reprogramming

scholarly journals Direct reprogramming of fibroblasts into endothelial cells capable of angiogenesis and reendothelialization in tissue-engineered vessels

2012 ◽  
Vol 109 (34) ◽  
pp. 13793-13798 ◽  
Author(s):  
A. Margariti ◽  
B. Winkler ◽  
E. Karamariti ◽  
A. Zampetaki ◽  
T.-n. Tsai ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Direct Reprogramming

scholarly journals Direct Reprogramming of Human Dermal Fibroblasts Into Endothelial Cells Using ER71/ETV2

2017 ◽  
Vol 120 (5) ◽  
pp. 848-861 ◽  
Author(s):  
Sangho Lee ◽  
Changwon Park ◽  
Ji Woong Han ◽  
Ju Young Kim ◽  
Kyuwon Cho ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Direct Reprogramming

scholarly journals Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state

2015 ◽  
Vol 10 (12) ◽  
pp. 1975-1985 ◽  
Author(s):  
Michael Ginsberg ◽  
William Schachterle ◽  
Koji Shido ◽  
Shahin Rafii
Keyword(s):  
Endothelial Cells ◽  
Direct Conversion ◽  
Pluripotent State ◽  
Amniotic Cells

20 Direct reprogramming fibroblasts into endothelial cells

Heart ◽  
2011 ◽  
Vol 97 (20) ◽  
pp. e7-e7
Author(s):  
A. Margariti ◽  
B. Winkler ◽  
E. Karamariti ◽  
T. Tsai ◽  
L. Zeng ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Direct Reprogramming

scholarly journals Direct reprogramming into endothelial cells: a new source for vascular regeneration

2017 ◽  
Vol 12 (4) ◽  
pp. 317-320 ◽  
Author(s):  
Sangho Lee ◽  
Jin Eyun Kim ◽  
Brandon AL Johnson ◽  
Adinarayana Andukuri ◽  
Young-Sup Yoon
Keyword(s):  
Endothelial Cells ◽  
Direct Reprogramming ◽  
Vascular Regeneration

scholarly journals Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Simone Bersini ◽  
Roberta Schulte ◽  
Ling Huang ◽  
Hannah Tsai ◽  
Martin W Hetzer
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Vascular Endothelial Cells ◽  
Functional Assay ◽  
Vascular Endothelial ◽  
Vascular Aging ◽  
Direct Reprogramming ◽  
Progeria Syndrome ◽  
Hutchinson Gilford Progeria Syndrome

Vascular dysfunctions are a common feature of multiple age-related diseases. However, modeling healthy and pathological aging of the human vasculature represents an unresolved experimental challenge. Here, we generated induced vascular endothelial cells (iVECs) and smooth muscle cells (iSMCs) by direct reprogramming of healthy human fibroblasts from donors of different ages and Hutchinson-Gilford Progeria Syndrome (HGPS) patients. iVECs induced from old donors revealed upregulation of GSTM1 and PALD1, genes linked to oxidative stress, inflammation and endothelial junction stability, as vascular aging markers. A functional assay performed on PALD1 KD VECs demonstrated a recovery in vascular permeability. We found that iSMCs from HGPS donors overexpressed bone morphogenetic protein (BMP)−4, which plays a key role in both vascular calcification and endothelial barrier damage observed in HGPS. Strikingly, BMP4 concentrations are higher in serum from HGPS vs. age-matched mice. Furthermore, targeting BMP4 with blocking antibody recovered the functionality of the vascular barrier in vitro, hence representing a potential future therapeutic strategy to limit cardiovascular dysfunction in HGPS. These results show that iVECs and iSMCs retain disease-related signatures, allowing modeling of vascular aging and HGPS in vitro.

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