Amniotic Fluid Stem Cells for the Treatment of Articular Cartilage Defects

2014 ◽  
pp. 87-97
Author(s):  
Andrea Preitschopf ◽  
Julia Busch ◽  
Hannes Zwickl ◽  
Stefan Nehrer ◽  
Markus Hengstschläger ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Amniotic Fluid ◽  
Cartilage Defects ◽  
Amniotic Fluid Stem Cells ◽  
Articular Cartilage Defects

scholarly journals A composite scaffold of Wharton’s jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee

2021 ◽  
Vol 32 (4) ◽  
Author(s):  
Zhong Li ◽  
Yikang Bi ◽  
Qi Wu ◽  
Chao Chen ◽  
Lu Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Composite Scaffold ◽  
Biomechanical Properties ◽  
Cartilage Defects ◽  
Human Umbilical Cord ◽  
Composite Scaffolds ◽  
Articular Cartilage Defects

AbstractTo evaluate the performance of a composite scaffold of Wharton’s jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out. The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold. In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee. Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs. We found that in vitro, the Young’s modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold. In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs. Both the scaffold and hUCMSCs showed low immunogenicity. These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.

Repair of Articular Cartilage Defects Using Mesenchymal Stem Cells

1995 ◽  
Vol 1 (4) ◽  
pp. 345-353 ◽  
Author(s):  
Daniel A. Grande ◽  
Sheila S. Southerland ◽  
Ryhanna Manji ◽  
Donald W. Pate ◽  
Robert E. Schwartz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Cartilage Defects ◽  
Articular Cartilage Defects

Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model

2016 ◽  
Vol 34 (11) ◽  
pp. 543-549 ◽  
Author(s):  
Reza Fekrazad ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Arman M. Shayan ◽  
Katayoun A.M. Kalhori ◽  
Fatemeh Mashhadi Abbas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Rabbit Model ◽  
Cartilage Defects ◽  
Articular Cartilage Defects

scholarly journals Repair of Articular Cartilage Defects using Bone Marrow Derived Mesenchymal Stem Cells in Rabbits

2019 ◽  
Vol 3 (1) ◽  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Single Dose ◽  
Articular Cartilage ◽  
Articular Surface ◽  
Cartilage Defects ◽  
Group Iii ◽  
Marrow Mesenchymal Stem Cells ◽  
Articular Cartilage Defects

The present study was performed to examine the effect of intra- articular injection of bone marrow mesenchymal stem cells (BMMSCs) and chondrogenic differentiated mesenchymal stem cells (CD- MSCs) on the repair of articular cartilage defects in rabbits. Twenty-five adult female baladi rabbits were used in this work. 5 rabbits were used for preparation of bone marrow mesenchymal stem cells (BM-MSCs) and their left knees were not subjected for the surgical procedure and used as normal control group. The remaining twenty rabbits were subjected for surgically induced cartilage defects in their left knees through a small medial parapatellar incision using bone curette. In the next day, the rabbits were divided into four groups: group I was not injected intraarticularly, group II injected intra-articularly by a single dose of saline, group III injected intra-articularly by a single dose of BM-MSCs and group IV injected intra-articularly by a single dose of CD-MSCs. After 8 weeks from the time of intra-articular injection. On time the rabbits were sacrificed and the entire knee joints were excised and examined. Groups I and II showed marked degenerative changes in their articular cartilage. The articular surface healed by fibrocartilage in group III, while in group IV the articular surface healed by hyaline cartilage. Treatment by CD-MSCs promotes a better healing effect on the articular cartilage defects of injured knee joints in rabbit’s model and has a remarkable superiority of repair than BM-MSCs. This can prevent the progress of cartilage defect into osteoarthritis which was a harmful disease.

Human Placenta-Derived Mesenchymal Stem Cells with Silk Fibroin Biomaterial in the Repair of Articular Cartilage Defects

2012 ◽  
Vol 14 (4) ◽  
pp. 334-341 ◽  
Author(s):  
Fang Li ◽  
Yong-Zhen Chen ◽  
Zong-Ning Miao ◽  
Shi-ying Zheng ◽  
Jun Jin
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Silk Fibroin ◽  
Human Placenta ◽  
Cartilage Defects ◽  
Articular Cartilage Defects
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