Optogenetics: Optical Control of a Photoactivatable Rac in Living Cells

2014 ◽  
pp. 277-289 ◽  
Author(s):  
Taofei Yin ◽  
Yi I. Wu
Keyword(s):  
Living Cells ◽  
Optical Control

scholarly journals Optical Control of Fast and Processive Engineered Myosins: Optimization and Characterization in Vitro and in Living Cells

2018 ◽  
Vol 114 (3) ◽  
pp. 318a
Author(s):  
Paul V. Ruijgrok ◽  
Rajarshi P. Ghosh ◽  
Muneaki Nakamura ◽  
Robert Chen ◽  
Vipul Vachharajani ◽  
...  
Keyword(s):  
Living Cells ◽  
Optical Control

scholarly journals Photoswitchable paclitaxel-based microtubule stabilisers allow optical control over the microtubule cytoskeleton

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Adrian Müller-Deku ◽  
Joyce C. M. Meiring ◽  
Kristina Loy ◽  
Yvonne Kraus ◽  
Constanze Heise ◽  
...  
Keyword(s):  
Intracellular Transport ◽  
Biological Response ◽  
Living Cells ◽  
Optical Control ◽  
Microtubule Cytoskeleton ◽  
Microtubule Network ◽  
Cellular Processes ◽  
Non Invasive ◽  
Chemical Inhibitors ◽  
Neuronal Physiology

Abstract Small molecule inhibitors are prime reagents for studies in microtubule cytoskeleton research, being applicable across a range of biological models and not requiring genetic engineering. However, traditional chemical inhibitors cannot be experimentally applied with spatiotemporal precision suiting the length and time scales inherent to microtubule-dependent cellular processes. We have synthesised photoswitchable paclitaxel-based microtubule stabilisers, whose binding is induced by photoisomerisation to their metastable state. Photoisomerising these reagents in living cells allows optical control over microtubule network integrity and dynamics, cell division and survival, with biological response on the timescale of seconds and spatial precision to the level of individual cells within a population. In primary neurons, they enable regulation of microtubule dynamics resolved to subcellular regions within individual neurites. These azobenzene-based microtubule stabilisers thus enable non-invasive, spatiotemporally precise modulation of the microtubule cytoskeleton in living cells, and promise new possibilities for studying intracellular transport, cell motility, and neuronal physiology.

scholarly journals Conjugated polymers for the optical control of the electrical activity of living cells

2016 ◽  
Vol 4 (31) ◽  
pp. 5272-5283 ◽  
Author(s):  
Susana Vaquero ◽  
Caterina Bossio ◽  
Sebastiano Bellani ◽  
Nicola Martino ◽  
Elena Zucchetti ◽  
...  
Keyword(s):  
Electrical Activity ◽  
Conjugated Polymers ◽  
Living Cells ◽  
Optical Control ◽  
Optical Coupling ◽  
Thermal Sterilization

Different conjugated polymers are proposed as bio-optical interfaces. Selected polymers are capable to sustain thermal sterilization but provide different optical coupling with living cells.

scholarly journals Optical Control of Fast and Processive Engineered Myosins In Vitro and in Living Cells

2019 ◽  
Vol 116 (3) ◽  
pp. 259a
Author(s):  
Paul V. Ruijgrok ◽  
Rajarshi P. Ghosh ◽  
Muneaki Nakamura ◽  
Sasha Zemsky ◽  
Robert Chen ◽  
...  
Keyword(s):  
Living Cells ◽  
Optical Control

scholarly journals Optical control of mitochondrial reductive reactions in living cells using an electron donor–acceptor linked molecule

Nanoscale ◽  
2017 ◽  
Vol 9 (47) ◽  
pp. 18690-18698 ◽  
Author(s):  
Yuta Takano ◽  
Reina Munechika ◽  
Vasudevanpillai Biju ◽  
Hideyoshi Harashima ◽  
Hiroshi Imahori ◽  
...  
Keyword(s):  
Electron Donor ◽  
Living Cells ◽  
Optical Control ◽  
Donor Acceptor ◽  
Selective Accumulation

A conjugate of a carrier and donor/acceptor linked molecules demonstrated a selective accumulation in mitochondria and a successful photoinduced reductive reaction.

scholarly journals Optical Control of Living Cells Electrical Activity by Conjugated Polymers

10.3791/53494 ◽  
2016 ◽  
Author(s):  
Nicola Martino ◽  
Caterina Bossio ◽  
Susana Vaquero Morata ◽  
Guglielmo Lanzani ◽  
Maria Rosa Antognazza
Keyword(s):  
Electrical Activity ◽  
Conjugated Polymers ◽  
Living Cells ◽  
Optical Control

Optical control of fast and processive engineered myosins in vitro and in living cells

2021 ◽  
Vol 17 (5) ◽  
pp. 540-548 ◽  
Author(s):  
Paul V. Ruijgrok ◽  
Rajarshi P. Ghosh ◽  
Sasha Zemsky ◽  
Muneaki Nakamura ◽  
Rui Gong ◽  
...  
Keyword(s):  
Living Cells ◽  
Optical Control
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