scholarly journals Optical Control of Living Cells Electrical Activity by Conjugated Polymers

10.3791/53494 ◽  
2016 ◽  
Author(s):  
Nicola Martino ◽  
Caterina Bossio ◽  
Susana Vaquero Morata ◽  
Guglielmo Lanzani ◽  
Maria Rosa Antognazza
Keyword(s):  
Electrical Activity ◽  
Conjugated Polymers ◽  
Living Cells ◽  
Optical Control

scholarly journals Conjugated polymers for the optical control of the electrical activity of living cells

2016 ◽  
Vol 4 (31) ◽  
pp. 5272-5283 ◽  
Author(s):  
Susana Vaquero ◽  
Caterina Bossio ◽  
Sebastiano Bellani ◽  
Nicola Martino ◽  
Elena Zucchetti ◽  
...  
Keyword(s):  
Electrical Activity ◽  
Conjugated Polymers ◽  
Living Cells ◽  
Optical Control ◽  
Optical Coupling ◽  
Thermal Sterilization

Different conjugated polymers are proposed as bio-optical interfaces. Selected polymers are capable to sustain thermal sterilization but provide different optical coupling with living cells.

Biomems Microprobe for Electrical Activity Recording of Living Cells

2007 ◽  
Author(s):  
C. Moldovan ◽  
R. Iosub ◽  
C.P. Lungu ◽  
A.M. Lungu ◽  
B. Firtat ◽  
...  
Keyword(s):  
Electrical Activity ◽  
Living Cells

scholarly journals A New Method to Study the Lysomal Electrical Activity in Living Cells

2018 ◽  
Vol 114 (3) ◽  
pp. 494a
Author(s):  
Ella Matamala ◽  
Cristian Castillo ◽  
Kirill Kiselyov ◽  
Sebastian Brauchi
Keyword(s):  
Electrical Activity ◽  
Living Cells ◽  
New Method

scholarly journals Optical Control of Fast and Processive Engineered Myosins: Optimization and Characterization in Vitro and in Living Cells

2018 ◽  
Vol 114 (3) ◽  
pp. 318a
Author(s):  
Paul V. Ruijgrok ◽  
Rajarshi P. Ghosh ◽  
Muneaki Nakamura ◽  
Robert Chen ◽  
Vipul Vachharajani ◽  
...  
Keyword(s):  
Living Cells ◽  
Optical Control

scholarly journals Photoswitchable paclitaxel-based microtubule stabilisers allow optical control over the microtubule cytoskeleton

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Adrian Müller-Deku ◽  
Joyce C. M. Meiring ◽  
Kristina Loy ◽  
Yvonne Kraus ◽  
Constanze Heise ◽  
...  
Keyword(s):  
Intracellular Transport ◽  
Biological Response ◽  
Living Cells ◽  
Optical Control ◽  
Microtubule Cytoskeleton ◽  
Microtubule Network ◽  
Cellular Processes ◽  
Non Invasive ◽  
Chemical Inhibitors ◽  
Neuronal Physiology

Abstract Small molecule inhibitors are prime reagents for studies in microtubule cytoskeleton research, being applicable across a range of biological models and not requiring genetic engineering. However, traditional chemical inhibitors cannot be experimentally applied with spatiotemporal precision suiting the length and time scales inherent to microtubule-dependent cellular processes. We have synthesised photoswitchable paclitaxel-based microtubule stabilisers, whose binding is induced by photoisomerisation to their metastable state. Photoisomerising these reagents in living cells allows optical control over microtubule network integrity and dynamics, cell division and survival, with biological response on the timescale of seconds and spatial precision to the level of individual cells within a population. In primary neurons, they enable regulation of microtubule dynamics resolved to subcellular regions within individual neurites. These azobenzene-based microtubule stabilisers thus enable non-invasive, spatiotemporally precise modulation of the microtubule cytoskeleton in living cells, and promise new possibilities for studying intracellular transport, cell motility, and neuronal physiology.

Fluorescence Ratiometric Assay Strategy for Chemical Transmitter of Living Cells Using H2O2-Sensitive Conjugated Polymers

2015 ◽  
Vol 7 (43) ◽  
pp. 24110-24118 ◽  
Author(s):  
Yunxia Wang ◽  
Shengliang Li ◽  
Liheng Feng ◽  
Chenyao Nie ◽  
Libing Liu ◽  
...  
Keyword(s):  
Conjugated Polymers ◽  
Living Cells ◽  
Ratiometric Assay

scholarly journals Optical Control of Fast and Processive Engineered Myosins In Vitro and in Living Cells

2019 ◽  
Vol 116 (3) ◽  
pp. 259a
Author(s):  
Paul V. Ruijgrok ◽  
Rajarshi P. Ghosh ◽  
Muneaki Nakamura ◽  
Sasha Zemsky ◽  
Robert Chen ◽  
...  
Keyword(s):  
Living Cells ◽  
Optical Control

scholarly journals Imaging the electrical activity of organelles in living cells

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Ella Matamala ◽  
Cristian Castillo ◽  
Juan P. Vivar ◽  
Patricio A. Rojas ◽  
Sebastian E. Brauchi
Keyword(s):  
Electrical Activity ◽  
Living Cells ◽  
Eukaryotic Cells ◽  
Subcellular Targeting ◽  
Spatiotemporal Resolution ◽  
Non Invasive ◽  
Optical Multiplexing ◽  
Voltage Dependent ◽  
Membrane Bound ◽  
Fret Pair

AbstractEukaryotic cells are complex systems compartmentalized in membrane-bound organelles. Visualization of organellar electrical activity in living cells requires both a suitable reporter and non-invasive imaging at high spatiotemporal resolution. Here we present hVoSorg, an optical method to monitor changes in the membrane potential of subcellular membranes. This method takes advantage of a FRET pair consisting of a membrane-bound voltage-insensitive fluorescent donor and a non-fluorescent voltage-dependent acceptor that rapidly moves across the membrane in response to changes in polarity. Compared to the currently available techniques, hVoSorg has advantages including simple and precise subcellular targeting, the ability to record from individual organelles, and the potential for optical multiplexing of organellar activity.

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