Analysis of Gene Expression in Neurons and Synapses by Multi-color In Situ Hybridization

2015 ◽  
pp. 293-317 ◽  
Author(s):  
Leonid L. Moroz ◽  
Andrea B. Kohn
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization

scholarly journals RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species

2021 ◽  
Vol 4 (1) ◽  
pp. 20
Author(s):  
Mujeeb Shittu ◽  
Tessa Steenwinkel ◽  
William Dion ◽  
Nathan Ostlund ◽  
Komal Raja ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Expression Patterns ◽  
Drosophila Species ◽  
Gene Expression Patterns ◽  
Probe Design ◽  
Tissue Preparation ◽  
Design And Synthesis ◽  
Rna In Situ Hybridization

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.

scholarly journals FISHing for chick genes: Triple-label whole-mount fluorescence in situ hybridization detects simultaneous and overlapping gene expression in avian embryos

2004 ◽  
Vol 229 (3) ◽  
pp. 651-657 ◽  
Author(s):  
Nathaniel Denkers ◽  
Pilar García-Villalba ◽  
Christopher K. Rodesch ◽  
Kandice R. Nielson ◽  
Teri Jo Mauch
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Avian Embryos ◽  
Overlapping Gene ◽  
Whole Mount

Ionotropic glutamate-receptor gene expression in hypothalamus: Localization of AMPA, kainate, and NMDA receptor RNA with in situ hybridization

1994 ◽  
Vol 343 (3) ◽  
pp. 428-444 ◽  
Author(s):  
Anthony N. Van Den Pol ◽  
Irm Hermans-Borgmeyer ◽  
Magdalena Hofer ◽  
Prabhat Ghosh ◽  
Stephen Heinemann
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Nmda Receptor ◽  
Glutamate Receptor ◽  
Receptor Gene ◽  
Ionotropic Glutamate Receptor ◽  
Receptor Gene Expression

scholarly journals Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.

1991 ◽  
Vol 88 (18) ◽  
pp. 8154-8158 ◽  
Author(s):  
A. M. Henney ◽  
P. R. Wakeley ◽  
M. J. Davies ◽  
K. Foster ◽  
R. Hembry ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Atherosclerotic Plaques

Postnatal ontogeny of POMC gene expression in the rat pituitary: an analysis by in situ hybridization histochemistry

1989 ◽  
Vol 47 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Ya-Qi Wang ◽  
Makoto Sato ◽  
Yasuhiro Morita ◽  
Koichi Noguchi ◽  
Hiroshi Kiyama ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Postnatal Ontogeny ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
Rat Pituitary ◽  
Pomc Gene

Detection of Myeloperoxidase Gene Expression in Minimally Differentiated Acute Myelogenous Leukemia (AML-M0) Using In Situ Hybridization

1995 ◽  
Vol 4 (3) ◽  
pp. 212-219 ◽  
Author(s):  
S. Thomas Traweek ◽  
Jane Liu ◽  
Rita M. Braziel ◽  
Rochelle M. Johnson ◽  
Russell K. Brynes
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Acute Myelogenous Leukemia ◽  
Myelogenous Leukemia ◽  
Acute Myelogenous
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