Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells upon Enteropathogenic Escherichia coli Infection

2015 ◽  
pp. 353-371
Author(s):  
Victor Yashunsky ◽  
Benjamin Aroeti
Keyword(s):  
Escherichia Coli ◽  
Surface Plasmon ◽  
Host Cells ◽  
Enteropathogenic Escherichia Coli ◽  
Escherichia Coli Infection ◽  
Surface Plasmon Spectroscopy

Interactions Between Salmonella Typhimurium, Enteropathogenic Escherichia Coli (EPEC), and Host Epithelial Cells

1995 ◽  
Vol 9 (1) ◽  
pp. 31-36 ◽  
Author(s):  
B.B. Finlay
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Salmonella Typhimurium ◽  
Host Cell ◽  
Inositol Phosphate ◽  
Enteric Pathogens ◽  
Host Protein ◽  
Host Cells ◽  
Enteropathogenic Escherichia Coli ◽  
Sequence Of Events

The interactions that occur between pathogenic micro-organisms and their host cells are complex and intimate. We have used two enteric pathogens, Salmonella typhimurium and enteropathogenic Escherichia coli (EPEC), to examine the interactions that occur between these organisms and epithelial cells. Although these are enteric pathogens, the knowledge and techniques developed from these systems may be applied to the study of dental pathogens. Both S. typhimurium and EPEC disrupt epithelial monolayer integrity, although by different mechanisms. Both pathogens cause loss of microvilli and re-arrangement of the underlying host cytoskeleton. Despite these similarities, both organisms send different signals into the host cell. EPEC signal transduction involves generation of intracellular calcium and inositol phosphate fluxes, and activation of host tyrosine kinases that results in tyrosine phosphorylation of a 90-kDa host protein. Bacterial mutants have been identifed that are deficient in signaling to the host. We propose a sequence of events that occur when EPEC interacts with epithelial cells. Once inside a host cell, S. typhimurium remains within a vacuole. To define some of the parameters of the intracellular environment, we constructed genetic fusions of known genes with lacZ, and used these fusions as reporter probes of the intracellular vacuolar environment. We have also begun to examine the bacterial and host cell factors necessary for S. typhimurium to multiply within epithelial cells. We found that this organism triggers the formation of novel tubular lysosomes, and these structures are linked with intracellular replication.

Enteropathogenic Escherichia coli Infection Inhibits Intestinal Ascorbic Acid Uptake via Dysregulation of Its Transporter Expression

2020 ◽  
Author(s):  
Christopher W. Heskett ◽  
Trevor Teafatiller ◽  
Carly Hennessey ◽  
Melanie G. Gareau ◽  
Jonathan S. Marchant ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ascorbic Acid ◽  
Acid Uptake ◽  
Enteropathogenic Escherichia Coli ◽  
Escherichia Coli Infection ◽  
Transporter Expression

Salmonella and Enteropathogenic Escherichia coli Interactions with Host Cells: Signaling Pathways

EcoSal Plus ◽  
2006 ◽  
Vol 2 (1) ◽  
Author(s):  
Danika L. Goosney ◽  
Sonya L. Kujat Choy ◽  
B. Brett Finlay
Keyword(s):  
Escherichia Coli ◽  
Signaling Pathways ◽  
Host Cells ◽  
Enteropathogenic Escherichia Coli

EscI: a crucial component of the type III secretion system forms the inner rod structure in enteropathogenic Escherichia coli

2012 ◽  
Vol 442 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Neta Sal-Man ◽  
Wanyin Deng ◽  
B. Brett Finlay
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Integral Membrane Protein ◽  
Secretion System ◽  
Host Cells ◽  
Enteropathogenic Escherichia Coli ◽  
Bacterial Membranes ◽  
Type Iii ◽  
Protein Functions

The T3SS (type III secretion system) is a multi-protein complex that plays a central role in the virulence of many Gram-negative bacterial pathogens. This apparatus spans both bacterial membranes and transports virulence factors from the bacterial cytoplasm into eukaryotic host cells. The T3SS exports substrates in a hierarchical and temporal manner. The first secreted substrates are the rod/needle proteins which are incorporated into the T3SS apparatus and are required for the secretion of later substrates, the translocators and effectors. In the present study, we provide evidence that rOrf8/EscI, a poorly characterized locus of enterocyte effacement-encoded protein, functions as the inner rod protein of the T3SS of EPEC (enteropathogenic Escherichia coli). We demonstrate that EscI is essential for type III secretion and is also secreted as an early substrate of the T3SS. We found that EscI interacts with EscU, the integral membrane protein that is linked to substrate specificity switching, implicating EscI in the substrate-switching event. Furthermore, we showed that EscI self-associates and interacts with the outer membrane secretin EscC, further supporting its function as an inner rod protein. Overall, the results of the present study suggest that EscI is the YscI/PrgJ/MxiI homologue in the T3SS of attaching and effacing pathogens.

scholarly journals Enteropathogenic Escherichia coli Infection Inhibits Intestinal Serotonin Transporter Function and Expression

2009 ◽  
Vol 137 (6) ◽  
pp. 2074-2083 ◽  
Author(s):  
Ali Esmaili ◽  
Saad F. Nazir ◽  
Alip Borthakur ◽  
Dan Yu ◽  
Jerrold R. Turner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Serotonin Transporter ◽  
Enteropathogenic Escherichia Coli ◽  
Escherichia Coli Infection ◽  
Intestinal Serotonin
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