Determination and Analysis of Cellular Metabolic Changes by Noncanonical Hedgehog Signaling

Methods in Molecular Biology - Hedgehog Signaling Protocols ◽

10.1007/978-1-4939-2772-2_16 ◽

2015 ◽

pp. 187-198

Author(s):

Raffaele Teperino ◽

John Andrew Pospisilik

Keyword(s):

Hedgehog Signaling ◽

Metabolic Changes

Download Full-text

Paracrine Hedgehog Signaling Drives Metabolic Changes in Hepatocellular Carcinoma

Cancer Research ◽

10.1158/0008-5472.can-12-1068 ◽

2012 ◽

Vol 72 (24) ◽

pp. 6344-6350 ◽

Cited By ~ 35

Author(s):

Isaac S. Chan ◽

Cynthia D. Guy ◽

Yuping Chen ◽

Jiuyi Lu ◽

Marzena Swiderska-Syn ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Hedgehog Signaling ◽

Metabolic Changes

Download Full-text

A Cytochemical Study of Glucose-6-Phosphatase (G-6-PASE) in Cells Participating in Atherogenesis of Rabbit Aorta

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100115924 ◽

1975 ◽

Vol 33 ◽

pp. 460-461

Author(s):

Sidney D. Kobernick ◽

Edna A. Elfont ◽

Neddra L. Brooks

Keyword(s):

Lipid Metabolism ◽

New Zealand ◽

Aortic Wall ◽

Rabbit Aorta ◽

Plaque Formation ◽

Metabolic Changes ◽

Atherosclerotic Plaques ◽

Cytochemical Study ◽

Cellular Alteration ◽

New Zealand White Rabbits

This cytochemical study was designed to investigate early metabolic changes in the aortic wall that might lead to or accompany development of atherosclerotic plaques in rabbits. The hypothesis that the primary cellular alteration leading to plaque formation might be due to changes in either carbohydrate or lipid metabolism led to histochemical studies that showed elevation of G-6-Pase in atherosclerotic plaques of rabbit aorta. This observation initiated the present investigation to determine how early in plaque formation and in which cells this change could be observed.Male New Zealand white rabbits of approximately 2000 kg consumed normal diets or diets containing 0.25 or 1.0 gm of cholesterol per day for 10, 50 and 90 days. Aortas were injected jin situ with glutaraldehyde fixative and dissected out. The plaques were identified, isolated, minced and fixed for not more than 10 minutes. Incubation and postfixation proceeded as described by Leskes and co-workers.

Download Full-text