The Standard Membrane Feeding Assay: Advances Using Bioluminescence

2015 ◽  
pp. 101-112 ◽  
Author(s):  
Will J. R. Stone ◽  
Teun Bousema
Keyword(s):  
Feeding Assay ◽  
Membrane Feeding ◽  
Membrane Feeding Assay

scholarly journals Optimization of a Membrane Feeding Assay for Plasmodium vivax Infection in Anopheles albimanus

2016 ◽  
Vol 10 (6) ◽  
pp. e0004807 ◽  
Author(s):  
Andrés F. Vallejo ◽  
Kelly Rubiano ◽  
Andres Amado ◽  
Amy R. Krystosik ◽  
Sócrates Herrera ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Vivax Infection ◽  
Anopheles Albimanus ◽  
Feeding Assay ◽  
Membrane Feeding ◽  
Membrane Feeding Assay

Evaluation of the standard membrane feeding assay (SMFA) for the determination of malaria transmission-reducing activity using empirical data

Parasitology ◽  
2005 ◽  
Vol 131 (04) ◽  
pp. 582
Author(s):  
M. VAN DER KOLK ◽  
S. J. DE VLAS ◽  
A. SAUL ◽  
M. VAN DE VEGTE-BOLMER ◽  
W. M. ELING ◽  
...  
Keyword(s):  
Malaria Transmission ◽  
Empirical Data ◽  
Feeding Assay ◽  
Membrane Feeding ◽  
Reducing Activity ◽  
Membrane Feeding Assay

scholarly journals Transmission-blocking activity is determined by transmission-reducing activity and number of control oocysts in Plasmodium falciparum standard membrane-feeding assay

Vaccine ◽  
2016 ◽  
Vol 34 (35) ◽  
pp. 4145-4151 ◽  
Author(s):  
Kazutoyo Miura ◽  
Bruce J. Swihart ◽  
Bingbing Deng ◽  
Luwen Zhou ◽  
Thao P. Pham ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Feeding Assay ◽  
Transmission Blocking ◽  
Membrane Feeding ◽  
Blocking Activity ◽  
Reducing Activity ◽  
Membrane Feeding Assay

scholarly journals Measuring the blockade of malaria transmission – An analysis of the Standard Membrane Feeding Assay

2012 ◽  
Vol 42 (11) ◽  
pp. 1037-1044 ◽  
Author(s):  
Thomas S. Churcher ◽  
Andrew M. Blagborough ◽  
Michael Delves ◽  
Chandra Ramakrishnan ◽  
Melissa C. Kapulu ◽  
...  
Keyword(s):  
Malaria Transmission ◽  
Feeding Assay ◽  
Membrane Feeding ◽  
Membrane Feeding Assay

scholarly journals Functional Comparison of Plasmodium falciparum Transmission-Blocking Vaccine Candidates by the Standard Membrane-Feeding Assay

2013 ◽  
Vol 81 (12) ◽  
pp. 4377-4382 ◽  
Author(s):  
Kazutoyo Miura ◽  
Eizo Takashima ◽  
Bingbing Deng ◽  
Gregory Tullo ◽  
Ababacar Diouf ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Recombinant Proteins ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Feeding Assay ◽  
Transmission Blocking ◽  
Content Type ◽  
Membrane Feeding ◽  
Membrane Feeding Assay

ABSTRACTRecently, there has been a renewed interest in the development of transmission-blocking vaccines (TBV) againstPlasmodium falciparummalaria. While several candidate TBVs have been reported, studies directly comparing them in functional assays are limited. To this end, recombinant proteins of TBV candidates Pfs25, Pfs230, and PfHAP2 were expressed in the wheat germ cell-free expression system. Outbred CD-1 mice were immunized twice with the antigens. Two weeks after the second immunization, IgG levels were measured by enzyme-linked immunosorbent assay (ELISA), and IgG functionality was assessed by the standard membrane-feeding assay (SMFA) using culturedP. falciparumNF54 gametocytes andAnopheles stephensimosquitoes. All three recombinant proteins elicited similar levels of antigen-specific IgG judged by ELISA. When IgGs purified from pools of immune serum were tested at 0.75 mg/ml in the SMFA, all three IgGs showed 97 to 100% inhibition in oocyst intensity compared to control IgG. In two additional independent SMFA evaluations, anti-Pfs25, anti-Pfs230, and anti-PfHAP2 IgGs inhibited oocyst intensity in a dose-dependent manner. When all three data sets were analyzed, anti-Pfs25 antibody showed significantly higher inhibition than the other two antibodies (P< 0.001 for both), while there was no significant difference between the other two (P= 0.15). A proportion of plasma samples collected from adults living in an area of malaria endemicity in Mali recognized Pfs230 and PfHAP2. This is the first study showing that the HAP2 protein ofP. falciparumcan induce transmission-blocking antibody. The current study supports the possibility of using this system for a comparative study with multiple TBV candidates.

scholarly journals Strong concordance between percent inhibition in oocyst and sporozoite intensities in a Plasmodium falciparum standard membrane-feeding assay

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Kazutoyo Miura ◽  
Bruce J. Swihart ◽  
Bingbing Deng ◽  
Luwen Zhou ◽  
Thao P. Pham ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Feeding Assay ◽  
Percent Inhibition ◽  
Membrane Feeding ◽  
Membrane Feeding Assay
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