Membrane Feeding Assay to Determine the Infectiousness of Plasmodium vivax Gametocytes

2015 ◽  
pp. 93-99 ◽  
Author(s):  
Jetsumon Sattabongkot ◽  
Chalermpon Kumpitak ◽  
Kirakorn Kiattibutr
Keyword(s):  
Plasmodium Vivax ◽  
Feeding Assay ◽  
Membrane Feeding ◽  
Membrane Feeding Assay

scholarly journals Optimization of a Membrane Feeding Assay for Plasmodium vivax Infection in Anopheles albimanus

2016 ◽  
Vol 10 (6) ◽  
pp. e0004807 ◽  
Author(s):  
Andrés F. Vallejo ◽  
Kelly Rubiano ◽  
Andres Amado ◽  
Amy R. Krystosik ◽  
Sócrates Herrera ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Vivax Infection ◽  
Anopheles Albimanus ◽  
Feeding Assay ◽  
Membrane Feeding ◽  
Membrane Feeding Assay

scholarly journals Evaluation and modeling of direct membrane-feeding assay with Plasmodium vivax to support development of transmission blocking vaccines

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kazutoyo Miura ◽  
Bruce J. Swihart ◽  
Michael P. Fay ◽  
Chalermpon Kumpitak ◽  
Kirakorn Kiattibutr ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Feeding Assay ◽  
Transmission Blocking ◽  
Membrane Feeding ◽  
Membrane Feeding Assay ◽  
Transmission Blocking Vaccines

scholarly journals Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yongzhe Zhang ◽  
Fei Liu ◽  
Yan Zhao ◽  
Fan Yang ◽  
Jie Bai ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plasmodium Vivax ◽  
Recombinant Proteins ◽  
Expression System ◽  
Anopheles Dirus ◽  
Transmission Blocking ◽  
Yeast Expression System ◽  
Membrane Feeding ◽  
Reducing Activity ◽  
Membrane Feeding Assay

Abstract Background Plasmodium vivax transmission-blocking vaccines (TBVs) are receiving increasing attention. Based on excellent transmission-blocking activities of the PbPH (PBANKA_0417200) and PbSOP26 (PBANKA_1457700) antigens in Plasmodium berghei, their orthologs in P. vivax, PVX_098655 (PvPH) and PVX_101120 (PvSOP26), were selected for the evaluation of their potential as TBVs. Methods Fragments of PvPH (amino acids 22–304) and PvSOP26 (amino acids 30–272) were expressed in the yeast expression system. The recombinant proteins were used to immunize mice to obtain antisera. The transmission-reducing activities of these antisera were evaluated using the direct membrane feeding assay (DMFA) using Anopheles dirus mosquitoes and P. vivax clinical isolates. Results The recombinant proteins PvPH and PvSOP26 induced robust antibody responses in mice. The DMFA showed that the anti-PvSOP26 sera significantly reduced oocyst densities by 92.0 and 84.1% in two parasite isolates, respectively, whereas the anti-PvPH sera did not show evident transmission-reducing activity. The variation in the DMFA results was unlikely due to the genetic polymorphisms of the two genes since their respective sequences were identical in the clinical P. vivax isolates. Conclusion PvSOP26 could be a promising TBV candidate for P. vivax, which warrants further evaluation. Graphical Abstract

Evaluation of the standard membrane feeding assay (SMFA) for the determination of malaria transmission-reducing activity using empirical data

Parasitology ◽  
2005 ◽  
Vol 131 (04) ◽  
pp. 582
Author(s):  
M. VAN DER KOLK ◽  
S. J. DE VLAS ◽  
A. SAUL ◽  
M. VAN DE VEGTE-BOLMER ◽  
W. M. ELING ◽  
...  
Keyword(s):  
Malaria Transmission ◽  
Empirical Data ◽  
Feeding Assay ◽  
Membrane Feeding ◽  
Reducing Activity ◽  
Membrane Feeding Assay

Evaluation of two Plasmodium vivax sexual-stage antigens as transmission-blocking vaccine candidates

2021 ◽  
Author(s):  
Yongzhe Zhang ◽  
Fei Liu ◽  
Yan Zhao ◽  
Fan Yang ◽  
Jie Bai ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plasmodium Vivax ◽  
Recombinant Proteins ◽  
Expression System ◽  
Transmission Blocking ◽  
Yeast Expression System ◽  
Membrane Feeding ◽  
Reducing Activity ◽  
Membrane Feeding Assay ◽  
Transmission Blocking Vaccines

Abstract Background: Plasmodium vivax transmission-blocking vaccines (TBVs) have received high attention. PVX_098655 (PvPH) and PVX_101120 (PvSOP26) were predicted to be potential TBV antigens based on the studies of their orthologs in Plasmodium berghei. Methods: Fragments of PvPH (amino acids 22–304) and PvSOP26 (amino acids 30–272) were expressed in the yeast expression system. The recombinant proteins were used to immunize mice to obtain the antisera. The transmission-reducing activities of these antisera were evaluated using the standard membrane feeding assay (SMFA) using Anopheles dirus mosquitoes and P. vivax clinical isolates. Results: The recombinant proteins PvPH and PvSOP26 induced robust antibody responses in mice. With SMFA, the anti-PvSOP26 sera significantly reduced oocyst densities by 92.0% and 84.1% in two parasite isolates, while the anti-PvPH sera did not show evident transmission-reducing activity. Both PvPH and PvSOP26 showed limited gene polymorphisms in the clinical P. vivax isolates. Conclusion: PvSOP26 could be a promising TBV candidate for P. vivax.

scholarly journals Transmission-blocking activity is determined by transmission-reducing activity and number of control oocysts in Plasmodium falciparum standard membrane-feeding assay

Vaccine ◽  
2016 ◽  
Vol 34 (35) ◽  
pp. 4145-4151 ◽  
Author(s):  
Kazutoyo Miura ◽  
Bruce J. Swihart ◽  
Bingbing Deng ◽  
Luwen Zhou ◽  
Thao P. Pham ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Feeding Assay ◽  
Transmission Blocking ◽  
Membrane Feeding ◽  
Blocking Activity ◽  
Reducing Activity ◽  
Membrane Feeding Assay
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