scholarly journals Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells

2016 ◽  
pp. 203-212 ◽  
Author(s):  
Krista Rombouts ◽  
Vinicio Carloni
Keyword(s):  
Liver Cells

Arginine synthesis by hepatomas in vitro. II. Isolation and characterization of Morris hepatoma variants unable to convert ornithine to arginine, and modulation of urea-cycle enzymes by dexamethasone and cyclic-AMP

1984 ◽  
Vol 68 (1) ◽  
pp. 305-319
Author(s):  
S.J. Goss
Keyword(s):  
Cyclic Amp ◽  
Liver Cells ◽  
Isolation And Characterization ◽  
Rat Liver Cells ◽  
Morris Hepatoma ◽  
Ornithine Transcarbamoylase ◽  
Cellular Incorporation

‘77orn’, a derivative of the Morris rat hepatoma 7777, stably expresses high levels of ornithine transcarbamoylase (OTC) and carbamoylphosphate synthetase I (CPS-I), and is able to grow indefinitely in ornithine-medium (medium with ornithine in place of arginine). Variants that have lost this ability are isolated from 77orn by a ‘suicide’ selective technique dependent on the cellular incorporation of [3H]ornithine. These variants, which have reduced levels of CPS-I, or of both CPS-I and OTC, are shown to have developed multiple hormonal requirements; their enzyme deficiencies can be reversed by use of an appropriately supplemented medium. In particular, CPS-I is inducible by dexamethasone and dibutyryl-cyclic-AMP in combination. Cholera toxin can be used instead of cyclic-AMP, but then butyrate is additionally required if the induction is to be maintained in the long term. The use of these agents in excess can depress OTC. Several other hepatomas, and alos explanted foetal rat liver cells, have similar requirements for CPS-I expression. It is argued that multiple hormonal requirements for CPS-I production are normal in liver cells in vitro, and that hormone-independent hepatomas should be regarded as abnormal. The implications of this for the somatic cell genetic investigation of differentiation are briefly discussed.

Characterization of the ERK1/2 phosphorylation profile in human and fish liver cells upon exposure to chemicals of environmental concern

2021 ◽  
pp. 103749
Author(s):  
Bojana Stanic ◽  
Jelena Petrovic ◽  
Branka Basica ◽  
Sonja Kaisarevic ◽  
Kristin Schirmer ◽  
...  
Keyword(s):  
Environmental Concern ◽  
Liver Cells ◽  
Fish Liver

Nanocrystal formulations of a poorly soluble drug. 1. In vitro characterization of stability, stabilizer adsorption and uptake in liver cells

2017 ◽  
Vol 518 (1-2) ◽  
pp. 29-40 ◽  
Author(s):  
Kalle Sigfridsson ◽  
Urban Skantze ◽  
Pia Skantze ◽  
Svante Johansson ◽  
Iain Grant ◽  
...  
Keyword(s):  
Liver Cells ◽  
In Vitro Characterization ◽  
Poorly Soluble ◽  
Poorly Soluble Drug
Sign in / Sign up

Export Citation Format

Share Document