Characterization of isometallothioneins in Chang liver cells and the transient accumulation of metallothionein and glutathione during cell proliferation

1999 ◽  
pp. 117-123 ◽  
Author(s):  
Annemarie Willi ◽  
Peter E. Hunziker
Keyword(s):  
Cell Proliferation ◽  
Liver Cells ◽  
Chang Liver

scholarly journals Cell Proliferation and Motility Are Inhibited by G1 Phase Arrest in 15-kDa Selenoprotein-Deficient Chang Liver Cells

2015 ◽  
Vol 38 (5) ◽  
pp. 457-465 ◽  
Author(s):  
Jeyoung Bang ◽  
Jang Hoe Huh ◽  
Ji-Woon Na ◽  
Qiao Lu ◽  
Bradley A. Carlson ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Liver Cells ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest ◽  
Chang Liver

scholarly journals Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal Proteins

2021 ◽  
Vol 22 (9) ◽  
pp. 4550
Author(s):  
Chi Nguyen Quynh Ho ◽  
Minh Thi Tran ◽  
Chung Chinh Doan ◽  
Son Nghia Hoang ◽  
Diem Hong Tran ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Cycle Progression ◽  
Simulated Microgravity ◽  
Liver Cells ◽  
Cytoskeletal Proteins ◽  
Cycle Phase ◽  
Control Group ◽  
Cell Cycle Regulators ◽  
Chang Liver

Simulated microgravity (SMG) induced the changes in cell proliferation and cytoskeleton organization, which plays an important factor in various cellular processes. The inhibition in cell cycle progression has been considered to be one of the main causes of proliferation inhibition in cells under SMG, but their mechanisms are still not fully understood. This study aimed to evaluate the effects of SMG on the proliferative ability and cytoskeleton changes of Chang Liver Cells (CCL-13). CCL-13 cells were induced SMG by 3D clinostat for 72 h, while the control group were treated in normal gravity at the same time. The results showed that SMG reduced CCL-13 cell proliferation by an increase in the number of CCL-13 cells in G0/G1 phase. This cell cycle phase arrest of CCL-13 cells was due to a downregulation of cell cycle-related proteins, such as cyclin A1 and A2, cyclin D1, and cyclin-dependent kinase 6 (Cdk6). SMG-exposed CCL-13 cells also exhibited a downregulation of α-tubulin 3 and β-actin which induced the cytoskeleton reorganization. These results suggested that the inhibited proliferation of SMG-exposed CCL-13 cells could be associate with the attenuation of major cell cycle regulators and main cytoskeletal proteins.

CHARACTERIZATION OF HISTAMINE-INDUCED INCREASES IN INTRACELLULAR FREE Ca2+CONCENTRATIONS IN CHANG LIVER CELLS

2001 ◽  
Vol 21 (1) ◽  
pp. 1-9 ◽  
Author(s):  
J. S. Cheng ◽  
K. C. Lee ◽  
J. L. Wang ◽  
H. T. Chang ◽  
K. J. Chou ◽  
...  
Keyword(s):  
Liver Cells ◽  
Chang Liver

Evidence for and Characterization of a Liver Cell Proliferation Factor from Blood Plasma of Partially Hepatectomized Rats

1980 ◽  
Vol 12 (03) ◽  
pp. 94-96 ◽  
Author(s):  
M. Goldberg ◽  
W. Strecker ◽  
D. Feeny ◽  
G. Ruhenstroth-Bauer
Keyword(s):  
Cell Proliferation ◽  
Blood Plasma ◽  
Liver Cell

scholarly journals Mass Spectrometry-Based Proteomic Characterization of Cutaneous Melanoma Ectosomes Reveals the Presence of Cancer-Related Molecules

2020 ◽  
Vol 21 (8) ◽  
pp. 2934 ◽  
Author(s):  
Magdalena Surman ◽  
Sylwia Kędracka-Krok ◽  
Dorota Hoja-Łukowicz ◽  
Urszula Jankowska ◽  
Anna Drożdż ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Cell Lines ◽  
Cutaneous Melanoma ◽  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Starting Point ◽  
Novel Biomarkers

Cutaneous melanoma (CM) is an aggressive type of skin cancer for which effective biomarkers are still needed. Recently, the protein content of extracellular vesicles (ectosomes and exosomes) became increasingly investigated in terms of its functional role in CM and as a source of novel biomarkers; however, the data concerning the proteome of CM-derived ectosomes is very limited. We used the shotgun nanoLC–MS/MS approach to the profile protein content of ectosomes from primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cell lines. Additionally, the effect exerted by CM ectosomes on recipient cells was assessed in terms of cell proliferation (Alamar Blue assay) and migratory properties (wound healing assay). All cell lines secreted heterogeneous populations of ectosomes enriched in the common set of proteins. A total of 1507 unique proteins were identified, with many of them involved in cancer cell proliferation, migration, escape from apoptosis, epithelial–mesenchymal transition and angiogenesis. Isolated ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of different cancer-promoting molecules. Taken together, these results confirm the significant role of ectosomes in several biological processes leading to CM development and progression, and might be used as a starting point for further studies exploring their diagnostic and prognostic potential.

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