Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)–Fluorescence Analysis of Proteins

2016 ◽  
pp. 1-10 ◽  
Author(s):  
Isabelle Le Potier ◽  
Audrey Boutonnet ◽  
Vincent Ecochard ◽  
François Couderc
Keyword(s):  
Capillary Electrophoresis ◽  
Fluorescence Analysis ◽  
Instrumental Approaches

scholarly journals Miniaturized Analysis of Amylopectin Chain Length Distribution by Fluorescence-Assisted Capillary Electrophoresis (FACE) down to Single Starch Granules

2021 ◽  
Author(s):  
amandine pruvost ◽  
stanislas helle ◽  
nicolas szydlowski ◽  
Christian ROLANDO
Keyword(s):  
Capillary Electrophoresis ◽  
Chain Length ◽  
Solid Phase ◽  
Potato Starch ◽  
Relative Proportion ◽  
Length Distribution ◽  
Fluorescence Analysis ◽  
Starch Granules ◽  
Chain Length Distribution ◽  
Single Granule

In the present work, we developed a miniaturized method for determining amylopectin chain length distribution (CLD) by fluorescence-assisted capillary electrophoresis (FACE). The method relies on single granule entrapping into capillaries followed by direct starch gelatinization and amylopectin debranching on carbograph-based solid phase extraction (SPE) cartridges. Sample desalting on HypersepTM tips following APTS-labelling and the use of nanovials allowed for the fluorescence analysis of weakly diluted samples. Consequently, method sensitivity was improved by 500-fold which is compatible with the analysis of single potato starch granules. The method was implemented to determine CLD profiles of single starch granules ranging from 50 to 100 µm in diameter. In these experiments, the relative proportion of starch glucans of up to 30 degrees of polymerization (DP) could be quantified.

Capillary electrophoresis-laser induced fluorescence analysis of endogenous damage in mitochondrial and genomic DNA

2005 ◽  
Vol 26 (13) ◽  
pp. 2599-2607 ◽  
Author(s):  
Michaela Wirtz ◽  
Claus A. Schumann ◽  
Marc Schellenträger ◽  
Siegmar Gäb ◽  
Jochen vom Brocke ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Genomic Dna ◽  
Fluorescence Analysis ◽  
Laser Induced Fluorescence

Distance-Dependent Metal-Enhanced Quantum Dots Fluorescence Analysis in Solution by Capillary Electrophoresis and Its Application to DNA Detection

2011 ◽  
Vol 83 (11) ◽  
pp. 4103-4109 ◽  
Author(s):  
Yong-Qiang Li ◽  
Li-Yun Guan ◽  
Hai-Li Zhang ◽  
Jun Chen ◽  
Song Lin ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Capillary Electrophoresis ◽  
Dna Detection ◽  
Fluorescence Analysis

scholarly journals Miniaturized Analysis of Amylopectin Chain Length Distribution by Fluorescence-Assisted Capillary Electrophoresis (FACE) down to Single Starch Granules

2021 ◽  
Author(s):  
amandine pruvost ◽  
stanislas helle ◽  
nicolas szydlowski ◽  
Christian ROLANDO
Keyword(s):  
Capillary Electrophoresis ◽  
Chain Length ◽  
Solid Phase ◽  
Potato Starch ◽  
Relative Proportion ◽  
Length Distribution ◽  
Fluorescence Analysis ◽  
Starch Granules ◽  
Chain Length Distribution ◽  
Single Granule

In the present work, we developed a miniaturized method for determining amylopectin chain length distribution (CLD) by fluorescence-assisted capillary electrophoresis (FACE). The method relies on single granule entrapping into capillaries followed by direct starch gelatinization and amylopectin debranching on carbograph-based solid phase extraction (SPE) cartridges. Sample desalting on HypersepTM tips following APTS-labelling and the use of nanovials allowed for the fluorescence analysis of weakly diluted samples. Consequently, method sensitivity was improved by 500-fold which is compatible with the analysis of single potato starch granules. The method was implemented to determine CLD profiles of single starch granules ranging from 50 to 100 µm in diameter. In these experiments, the relative proportion of starch glucans of up to 30 degrees of polymerization (DP) could be quantified.

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