Online Capillary IsoElectric Focusing-ElectroSpray Ionization Mass Spectrometry (CIEF-ESI MS) in Glycerol–Water Media for the Separation and Characterization of Hydrophilic and Hydrophobic Proteins

2016 ◽  
pp. 57-66 ◽  
Author(s):  
Meriem Mokaddem ◽  
Fanny d’Orlyé ◽  
Anne Varenne
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Electrospray Ionization Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Capillary Isoelectric Focusing ◽  
Glycerol Water ◽  
Water Media

On-line capillary isoelectric focusing hyphenated to native electrospray ionization mass spectrometry for the characterization of interferon-γ and variants

The Analyst ◽  
2015 ◽  
Vol 140 (2) ◽  
pp. 543-550 ◽  
Author(s):  
Cédric Przybylski ◽  
Meriem Mokaddem ◽  
Mehdi Prull-Janssen ◽  
Els Saesen ◽  
Hugues Lortat-Jacob ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Electrospray Ionization Mass Spectrometry ◽  
Interferon Γ ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Capillary Isoelectric Focusing ◽  
Detection Mode ◽  
On Line

The on-line hyphenation of Capillary IsoElectric Focusing (CIEF) with ElectroSpray Ionization Mass Spectrometry (ESI/MS) has been carried out in a non-denaturing detection mode at the CIEF-MS interface.

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