The on-line hyphenation of Capillary IsoElectric Focusing (CIEF) with ElectroSpray Ionization Mass Spectrometry (ESI/MS) has been carried out in a non-denaturing detection mode at the CIEF-MS interface.
Efficient ‘on-thread’ isoelectric focusing of proteins, with direct on-thread detection using desorption electrospray ionisation mass spectrometry.