Characterization of the F(ab′)2 fragment of a murine monoclonal antibody using capillary isoelectric focusing and electrospray ionization mass spectrometry

1998 ◽  
Vol 816 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Marie-Luise Hagmann ◽  
Christine Kionka ◽  
Martin Schreiner ◽  
Christine Schwer
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Electrospray Ionization Mass Spectrometry ◽  
Murine Monoclonal Antibody ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Capillary Isoelectric Focusing

On-line capillary isoelectric focusing hyphenated to native electrospray ionization mass spectrometry for the characterization of interferon-γ and variants

The Analyst ◽  
2015 ◽  
Vol 140 (2) ◽  
pp. 543-550 ◽  
Author(s):  
Cédric Przybylski ◽  
Meriem Mokaddem ◽  
Mehdi Prull-Janssen ◽  
Els Saesen ◽  
Hugues Lortat-Jacob ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Electrospray Ionization Mass Spectrometry ◽  
Interferon Γ ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Capillary Isoelectric Focusing ◽  
Detection Mode ◽  
On Line

The on-line hyphenation of Capillary IsoElectric Focusing (CIEF) with ElectroSpray Ionization Mass Spectrometry (ESI/MS) has been carried out in a non-denaturing detection mode at the CIEF-MS interface.

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